##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AccIII AclI AflII AhdI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BbeI BbrPI BcgI BfrI
BlnI BsaAI BsaBI BseAI BsiWI
BspEI BspHI BssHII BssSI Bst1107I
BstBI BstZ17I CpoI DraI EagI
Eam1105I EclXI Eco52I EcoRI EcoRV
EcoT22I FseI FspI HpaI KasI
KspI MamI MfeI MluI MroI
MunI NarI NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshBI Psp1406I RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1834 GACGTC GACGT^C
AcsI 3198 AAATTT [GA]^AATT[CT]
AgeI 3136 ACCGGT A^CCGGT
Aor51HI 3608 AGCGCT AGC^GCT
ApoI 3198 AAATTT [GA]^AATT[CT]
AspI 3957 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 552 TGGCCA TGG^CCA
BbsI 724 GAAGAC GAAGAC(2/6)
BciVI 515 GTATCC GTATCC(6/5)
BglI 2697 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 724 GAAGAC GAAGAC(2/6)
BsiEI 940 CGATCG CG[GA][CT]^CG
BspDI 1391 ATCGAT AT^CGAT
ClaI 1391 ATCGAT AT^CGAT
Eco47III 3608 AGCGCT AGC^GCT
HincII 919 GTCAAC GT[CT]^[GA]AC
HindII 919 GTCAAC GT[CT]^[GA]AC
HindIII 866 AAGCTT A^AGCTT
MluNI 552 TGGCCA TGG^CCA
MscI 552 TGGCCA TGG^CCA
NdeI 3288 CATATG CA^TATG
NheI 273 GCTAGC G^CTAGC
PflFI 3957 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1542 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3136 ACCGGT A^CCGGT
PshAI 2632 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 940 CGATCG CGAT^CG
SexAI 4462 ACCTGGT A^CC[AT]GGT
SphI 4881 GCATGC GCATG^C
Tth111I 3957 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1542 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3333 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 428 GGTACC G^GTACC
3394 GGTACC
AccI 2551 GTCTAC GT^[AC][GT]AC
2639 GTCTAC
AcyI 1834 GACGTC G[GA]^CG[CT]C
1899 GACGCC
AflIII 1242 ACATGT A^C[GA][CT]GT
3297 ACATGT
Alw44I 1419 GTGCAC G^TGCAC
3488 GTGCAC
ApaI 382 GGGCCC GGGCC^C
3821 GGGCCC
ApaLI 1419 GTGCAC G^TGCAC
3488 GTGCAC
Asp718I 428 GGTACC G^GTACC
3394 GGTACC
BamHI 626 GGATCC G^GATCC
2219 GGATCC
BclI 639 TGATCA T^GATCA
4410 TGATCA
BglII 773 AGATCT A^GATCT
3851 AGATCT
BsaHI 1834 GACGTC G[GA]^CG[CT]C
1899 GACGCC
BsmBI 1897 GAGACG CGTCTC(1/5)
2150 GAGACG
Bsp1407I 175 TGTACA T^GTACA
3636 TGTACA
BspLU11I 1242 ACATGT A^CATGT
3297 ACATGT
BsrBI 124 CCGCTC CCGCTC(-3/-3)
1432 GAGCGG
BsrGI 175 TGTACA T^GTACA
3636 TGTACA
BstEII 2406 GGTAACC G^GT[ACGT]ACC
3080 GGTCACC
BstPI 2406 GGTAACC G^GT[ACGT]ACC
3080 GGTCACC
DraIII 1325 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3833 CACTGGGTG
DrdI 1648 GACTGGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1828 GACCCTGACGTC
EcoNI 1192 CCTTTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2670 CCTGGCCGAGG
EcoO65I 2406 GGTAACC G^GT[ACGT]ACC
3080 GGTCACC
FbaI 639 TGATCA T^GATCA
4410 TGATCA
HgaI 580 GACGC GACGC(5/10)
1899 GACGC
Hin1I 1834 GACGTC G[GA]^CG[CT]C
1899 GACGCC
KpnI 428 GGTACC GGTAC^C
3394 GGTACC
NaeI 841 GCCGGC GCC^GGC
971 GCCGGC
NgoMIV 841 GCCGGC G^CCGGC
971 GCCGGC
SmlI 4585 CTTGAG C^T[CT][GA]AG
4951 CTCAAG
SspBI 175 TGTACA T^GTACA
3636 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI