##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI Alw44I ApaI ApaLI AscI
AseI AsnI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BglI
BlnI BsaBI BsaHI BseAI BsiWI
BsmBI BspEI BssHII BssSI BstUI
CpoI DraI DraIII EcoRV FseI
FspI Hin1I HpaI KasI KspI
MamI MluI MroI MvnI NarI
NdeI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I RsrII SacII SalI SfiI
SfoI SgrAI SmaI SnaBI SspI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 4150 GGTACC G^GTACC
AhdI 1340 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4286 CCTGAGG CC^T[ACGT]AGG
Asp718I 4150 GGTACC G^GTACC
AspEI 1340 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1335 CCCGAG C^[CT]CG[GA]G
BciVI 2766 GTATCC GTATCC(6/5)
BclI 1665 TGATCA T^GATCA
BsaAI 149 CACGTA [CT]AC^GT[GA]
BsaWI 531 ACCGGA [AT]^CCGG[AT]
BsmI 1303 GAATGC GAATGC(1/-1)
BsoBI 1335 CCCGAG C^[CT]CG[GA]G
BspDI 4004 ATCGAT AT^CGAT
BspHI 4945 TCATGA T^CATGA
BstBI 645 TTCGAA TT^CGAA
BstEII 499 GGTCACC G^GT[ACGT]ACC
BstPI 499 GGTCACC G^GT[ACGT]ACC
Bsu36I 4286 CCTGAGG CC^T[ACGT]AGG
ClaI 4004 ATCGAT AT^CGAT
DraII 1845 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 4847 GACCCCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 209 CGGCCG C^GGCCG
Eam1105I 1340 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 209 CGGCCG C^GGCCG
Eco52I 209 CGGCCG C^GGCCG
Eco81I 4286 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1845 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 499 GGTCACC G^GT[ACGT]ACC
EcoRI 2581 GAATTC G^AATTC
EcoT22I 4472 ATGCAT ATGCA^T
FauI 1416 CCCGC CCCGC(4/6)
FbaI 1665 TGATCA T^GATCA
HgaI 4378 GACGC GACGC(5/10)
KpnI 4150 GGTACC GGTAC^C
NaeI 211 GCCGGC GCC^GGC
NgoMIV 211 GCCGGC G^CCGGC
NheI 4061 GCTAGC G^CTAGC
NsiI 4472 ATGCAT ATGCA^T
NspV 645 TTCGAA TT^CGAA
PflMI 5266 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1845 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2951 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1990 CGATCG CGAT^CG
RcaI 4945 TCATGA T^CATGA
SfuI 645 TTCGAA TT^CGAA
SpeI 3311 ACTAGT A^CTAGT
SphI 2445 GCATGC GCATG^C
Van91I 5266 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 5262 CCACCCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1291 ACATGT A^C[GA][CT]GT
3229 ACATGT
Aor51HI 2679 AGCGCT AGC^GCT
5349 AGCGCT
AspI 2378 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5270 GACATGGTC
Bsp1407I 375 TGTACA T^GTACA
518 TGTACA
BspLU11I 1291 ACATGT A^CATGT
3229 ACATGT
BsrBI 2711 GAGCGG CCGCTC(-3/-3)
5054 GAGCGG
BsrFI 211 GCCGGC [GA]^CCGG[CT]
1587 ACCGGC
BsrGI 375 TGTACA T^GTACA
518 TGTACA
Bst1107I 2093 GTATAC GTA^TAC
4710 GTATAC
BstAPI 1226 GCATATAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2143 GCAGAGACTGC
BstZ17I 2093 GTATAC GTA^TAC
4710 GTATAC
Cfr10I 211 GCCGGC [GA]^CCGG[CT]
1587 ACCGGC
DsaI 1443 CCATGG C^C[GA][CT]GG
2800 CCATGG
Eco47III 2679 AGCGCT AGC^GCT
5349 AGCGCT
MaeII 150 ACGT A^CGT
2298 ACGT
MfeI 2424 CAATTG C^AATTG
5079 CAATTG
MunI 2424 CAATTG C^AATTG
5079 CAATTG
NcoI 1443 CCATGG C^CATGG
2800 CCATGG
PflFI 2378 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5270 GACATGGTC
SacI 3692 GAGCTC GAGCT^C
5537 GAGCTC
SbfI 484 CCTGCAGG CCTGCA^GG
4780 CCTGCAGG
ScaI 444 AGTACT AGT^ACT
2976 AGTACT
Sse8387I 484 CCTGCAGG CCTGCA^GG
4780 CCTGCAGG
SspBI 375 TGTACA T^GTACA
518 TGTACA
StuI 4785 AGGCCT AGG^CCT
4988 AGGCCT
Tth111I 2378 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5270 GACATGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BfrI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII EaeI EarI EcoNI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI