##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AccI AclI AgeI AhdI AscI
AspEI AspI AvrII BbvCI BcgI
BlnI BsiEI BsiWI BspDI BspLU11I
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoRI FseI NaeI NcoI
NdeI NgoMIV NotI NruI NspV
PaeR7I PflFI PinAI PshAI Psp1406I
PvuI RsrII SalI SfiI SfuI
SnaBI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 4116 TCCGGA T^CCGGA
Alw44I 2738 GTGCAC G^TGCAC
Aor51HI 3015 AGCGCT AGC^GCT
ApaI 10 GGGCCC GGGCC^C
ApaLI 2738 GTGCAC G^TGCAC
AseI 3005 ATTAAT AT^TAAT
AsnI 3005 ATTAAT AT^TAAT
BamHI 1402 GGATCC G^GATCC
BbeI 2436 GGCGCC GGCGC^C
BbrPI 4337 CACGTG CAC^GTG
BciVI 2428 GGATAC GTATCC(6/5)
BclI 750 TGATCA T^GATCA
BglI 4038 GCCATAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 4116 TCCGGA T^CCGGA
BsmBI 77 CGTCTC CGTCTC(1/5)
BspEI 4116 TCCGGA T^CCGGA
BsrDI 3508 CATTGC GCAATG(2/0)
BstAPI 4917 GCACAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 3320 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3015 AGCGCT AGC^GCT
FbaI 750 TGATCA T^GATCA
KasI 2436 GGCGCC G^GCGCC
KspI 33 CCGCGG CCGC^GG
MluI 2523 ACGCGT A^CGCGT
MroI 4116 TCCGGA T^CCGGA
NarI 2436 GGCGCC GG^CGCC
NheI 2939 GCTAGC G^CTAGC
PacI 830 TTAATTAA TTAAT^TAA
PmaCI 4337 CACGTG CAC^GTG
PmeI 6499 GTTTAAAC GTTT^AAAC
PmlI 4337 CACGTG CAC^GTG
PshBI 3005 ATTAAT AT^TAAT
SacII 33 CCGCGG CCGC^GG
SapI 523 GCTCTTC GCTCTTC(1/4)
SbfI 1906 CCTGCAGG CCTGCA^GG
SexAI 5147 ACCAGGT A^CC[AT]GGT
SfoI 2436 GGCGCC GGC^GCC
SgrAI 150 CACCGGCG C[GA]^CCGG[CT]G
SpeI 3146 ACTAGT A^CTAGT
SphI 1272 GCATGC GCATG^C
Sse8387I 1906 CCTGCAGG CCTGCA^GG
XbaI 1753 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 274 GGTACC G^GTACC
1846 GGTACC
AflII 4444 CTTAAG C^TTAAG
5594 CTTAAG
Asp718I 274 GGTACC G^GTACC
1846 GGTACC
AviII 1359 TGCGCA TGC^GCA
5859 TGCGCA
BalI 2811 TGGCCA TGG^CCA
4138 TGGCCA
BfrI 4444 CTTAAG C^TTAAG
5594 CTTAAG
BsgI 2030 CTGCAC GTGCAG(16/14)
2277 GTGCAG
BsrBI 86 CCGCTC CCGCTC(-3/-3)
187 GAGCGG
BsrFI 151 ACCGGC [GA]^CCGG[CT]
1030 GCCGGT
BssSI 893 CACGAG CACGAG(-5/-1)
2234 CACGAG
BstEII 313 GGTGACC G^GT[ACGT]ACC
1111 GGTCACC
BstPI 313 GGTGACC G^GT[ACGT]ACC
1111 GGTCACC
BstXI 4797 CCAAGGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6049 CCAATGTGATGG
Cfr10I 151 ACCGGC [GA]^CCGG[CT]
1030 GCCGGT
DsaI 33 CCGCGG C^C[GA][CT]GG
4192 CCACGG
EcoO65I 313 GGTGACC G^GT[ACGT]ACC
1111 GGTCACC
EcoRV 4257 GATATC GAT^ATC
5929 GATATC
EcoT22I 4713 ATGCAT ATGCA^T
5763 ATGCAT
FspI 1359 TGCGCA TGC^GCA
5859 TGCGCA
HpaI 1559 GTTAAC GTT^AAC
4940 GTTAAC
KpnI 274 GGTACC GGTAC^C
1846 GGTACC
MluNI 2811 TGGCCA TGG^CCA
4138 TGGCCA
MscI 2811 TGGCCA TGG^CCA
4138 TGGCCA
NsiI 4713 ATGCAT ATGCA^T
5763 ATGCAT
PflMI 1668 CCAATCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3990 CCAAGATCTGG
SacI 2930 GAGCTC GAGCT^C
5168 GAGCTC
ScaI 3195 AGTACT AGT^ACT
3246 AGTACT
Van91I 1668 CCAATCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3990 CCAAGATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MspI
MunI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI XmnI