##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccII AccIII AclI AflIII
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AviII AvrII BbeI
BbrPI BlnI BlpI Bpu1102I BsaAI
BseAI BsiWI BsmBI BspEI BspLU11I
BssHII BstUI BstXI CelII CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoT22I FseI FspI HaeII KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PmaCI PmeI PmlI PshAI
PshBI Psp1406I RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SnaBI SphI Sse8387I SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 2651 GACGTC GACGT^C
AcyI 2651 GACGTC G[GA]^CG[CT]C
AgeI 4449 ACCGGT A^CCGGT
AspI 455 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 137 TGGCCA TGG^CCA
BamHI 944 GGATCC G^GATCC
BbvCI 2550 CCTCAGC CCTCAGC(-5/-2)
BglII 4248 AGATCT A^GATCT
BsaHI 2651 GACGTC G[GA]^CG[CT]C
BsaWI 4449 ACCGGT [AT]^CCGG[AT]
BsiEI 53 CGATCG CG[GA][CT]^CG
Bsp1407I 1552 TGTACA T^GTACA
BspDI 666 ATCGAT AT^CGAT
BsrBI 2477 CCGCTC CCGCTC(-3/-3)
BsrGI 1552 TGTACA T^GTACA
BssSI 4691 CACGAG CACGAG(-5/-1)
Bst1107I 1559 GTATAC GTA^TAC
BstBI 3463 TTCGAA TT^CGAA
BstEII 2563 GGTCACC G^GT[ACGT]ACC
BstPI 2563 GGTCACC G^GT[ACGT]ACC
BstZ17I 1559 GTATAC GTA^TAC
CfoI 206 GCGC GCG^C
ClaI 666 ATCGAT AT^CGAT
DrdI 2548 GACCTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 137 TGGCCA [CT]^GGCC[GA]
EcoNI 2834 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2563 GGTCACC G^GT[ACGT]ACC
EcoRV 1389 GATATC GAT^ATC
HhaI 206 GCGC GCG^C
Hin1I 2651 GACGTC G[GA]^CG[CT]C
HinP1I 206 GCGC G^CGC
HpaI 692 GTTAAC GTT^AAC
MluNI 137 TGGCCA TGG^CCA
MscI 137 TGGCCA TGG^CCA
NcoI 1496 CCATGG C^CATGG
NdeI 4881 CATATG CA^TATG
NspV 3463 TTCGAA TT^CGAA
PflFI 455 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4449 ACCGGT A^CCGGT
PvuI 53 CGATCG CGAT^CG
SapI 2454 GCTCTTC GCTCTTC(1/4)
SfuI 3463 TTCGAA TT^CGAA
SmaI 1642 CCCGGG CCC^GGG
SpeI 2703 ACTAGT A^CTAGT
SspBI 1552 TGTACA T^GTACA
StuI 151 AGGCCT AGG^CCT
Tth111I 455 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1642 CCCGGG C^CCGGG
XmaI 1642 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 1239 GTCTAC GT^[AC][GT]AC
1559 GTATAC
AflII 644 CTTAAG C^TTAAG
4350 CTTAAG
AocI 211 CCTGAGG CC^T[ACGT]AGG
2917 CCTCAGG
ApaI 169 GGGCCC GGGCC^C
1916 GGGCCC
BanI 2735 GGCACC G^G[CT][GA]CC
4084 GGCACC
BcgI 1401 GCAGAAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2036 GCATCTACTTCG
BciVI 4325 GTATCC GTATCC(6/5)
4800 GTATCC
BfrI 644 CTTAAG C^TTAAG
4350 CTTAAG
Bsu36I 211 CCTGAGG CC^T[ACGT]AGG
2917 CCTCAGG
DraI 3992 TTTAAA TTT^AAA
5077 TTTAAA
DraIII 1503 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4822 CACCTTGTG
Eco81I 211 CCTGAGG CC^T[ACGT]AGG
2917 CCTCAGG
MaeII 540 ACGT A^CGT
2652 ACGT
MslI 3155 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4546 CACAGTGGTG
PstI 39 CTGCAG CTGCA^G
4842 CTGCAG
ScaI 2071 AGTACT AGT^ACT
2706 AGTACT
SspI 1842 AATATT AAT^ATT
3981 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI Asp700I
AspHI AvaI AvaII BanII BbsI
BbvI BclI BcnI BfaI BglI
BmyI BpmI BpuAI BsaBI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr10I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRI EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MamI MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI