################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## Acc65I AclI AgeI AscI Asp718I AviII AvrII BamHI BbeI BcgI BciVI BlnI BsiWI BsmBI BspDI BspMI BsrFI BssHII BstBI Cfr10I ClaI CpoI DraIII EagI EclXI Eco52I EcoRV EcoT22I FseI FspI HgaI HpaI KasI KpnI KspI MfeI MluI MunI NaeI NarI NgoMIV NotI NruI NsiI NspV PinAI PshAI Psp1406I RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 2496 GACGTC GACGT^C AccII 1854 CGCG CG^CG AcyI 2496 GACGTC G[GA]^CG[CT]C AhdI 98 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 207 AGCGCT AGC^GCT ApaI 4523 GGGCCC GGGCC^C AspEI 98 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 5324 TGGCCA TGG^CCA BbrPI 2725 CACGTG CAC^GTG BbvCI 4560 GCTGAGG CCTCAGC(-5/-2) BclI 5829 TGATCA T^GATCA BglI 3497 GCCAGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 2725 CACGTG [CT]AC^GT[GA] BsaHI 2496 GACGTC G[GA]^CG[CT]C BseRI 2667 CTCCTC GAGGAG(10/8) BsiEI 1907 CGATCG CG[GA][CT]^CG BsrDI 4860 GCAATG GCAATG(2/0) Bst1107I 4752 GTATAC GTA^TAC BstAPI 5368 GCAAACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1854 CGCG CG^CG BstZ17I 4752 GTATAC GTA^TAC Eam1105I 98 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 207 AGCGCT AGC^GCT EcoNI 4514 CCTAGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 4171 CCCGC CCCGC(4/6) FbaI 5829 TGATCA T^GATCA HaeII 207 AGCGCT [GA]GCGC^[CT] Hin1I 2496 GACGTC G[GA]^CG[CT]C HindIII 5079 AAGCTT A^AGCTT MluNI 5324 TGGCCA TGG^CCA MscI 5324 TGGCCA TGG^CCA MvnI 1854 CGCG CG^CG NdeI 5878 CATATG CA^TATG NheI 3151 GCTAGC G^CTAGC PmaCI 2725 CACGTG CAC^GTG PmeI 2869 GTTTAAAC GTTT^AAAC PmlI 2725 CACGTG CAC^GTG PvuI 1907 CGATCG CGAT^CG SacI 2365 GAGCTC GAGCT^C ScaI 2614 AGTACT AGT^ACT SexAI 4090 ACCTGGT A^CC[AT]GGT SphI 5312 GCATGC GCATG^C StuI 3786 AGGCCT AGG^CCT XbaI 2776 TCTAGA T^CTAGA XcmI 3498 CCAGTCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### Alw44I 1284 GTGCAC G^TGCAC 3478 GTGCAC AocI 3670 CCTGAGG CC^T[ACGT]AGG 5727 CCTGAGG ApaLI 1284 GTGCAC G^TGCAC 3478 GTGCAC AspI 194 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1056 GACCATGTC BglII 2005 AGATCT A^GATCT 4291 AGATCT BsaBI 131 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1251 GATGCTAATC BsrBI 1795 GAGCGG CCGCTC(-3/-3) 3257 GAGCGG BssSI 5614 CACGAG CACGAG(-5/-1) 6108 CTCGTG Bsu36I 3670 CCTGAGG CC^T[ACGT]AGG 5727 CCTGAGG DrdI 1744 GACAAGTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4055 GACCCCAGTGTC Eco81I 3670 CCTGAGG CC^T[ACGT]AGG 5727 CCTGAGG MamI 131 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1251 GATGCTAATC PacI 5489 TTAATTAA TTAAT^TAA 6481 TTAATTAA PaeR7I 3463 CTCGAG C^TCGAG 4552 CTCGAG PflFI 194 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1056 GACCATGTC SpeI 2611 ACTAGT A^CTAGT 2917 ACTAGT SwaI 4706 ATTTAAAT ATTT^AAAT 4742 ATTTAAAT Tth111I 194 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1056 GACCATGTC XhoI 3463 CTCGAG C^TCGAG 4552 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccI AccIII AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BfrI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseAI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspEI BspHI BspLU11I BsrGI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshBI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI