##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AclI AgeI AscI Asp718I
AviII AvrII BamHI BbeI BcgI
BciVI BlnI BsiWI BsmBI BspDI
BspMI BsrFI BssHII BstBI Cfr10I
ClaI CpoI DraIII EagI EclXI
Eco52I EcoRV EcoT22I FseI FspI
HgaI HpaI KasI KpnI KspI
MfeI MluI MunI NaeI NarI
NgoMIV NotI NruI NsiI NspV
PinAI PshAI Psp1406I RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2496 GACGTC GACGT^C
AccII 1854 CGCG CG^CG
AcyI 2496 GACGTC G[GA]^CG[CT]C
AhdI 98 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 207 AGCGCT AGC^GCT
ApaI 4523 GGGCCC GGGCC^C
AspEI 98 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 5324 TGGCCA TGG^CCA
BbrPI 2725 CACGTG CAC^GTG
BbvCI 4560 GCTGAGG CCTCAGC(-5/-2)
BclI 5829 TGATCA T^GATCA
BglI 3497 GCCAGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2725 CACGTG [CT]AC^GT[GA]
BsaHI 2496 GACGTC G[GA]^CG[CT]C
BseRI 2667 CTCCTC GAGGAG(10/8)
BsiEI 1907 CGATCG CG[GA][CT]^CG
BsrDI 4860 GCAATG GCAATG(2/0)
Bst1107I 4752 GTATAC GTA^TAC
BstAPI 5368 GCAAACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1854 CGCG CG^CG
BstZ17I 4752 GTATAC GTA^TAC
Eam1105I 98 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 207 AGCGCT AGC^GCT
EcoNI 4514 CCTAGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 4171 CCCGC CCCGC(4/6)
FbaI 5829 TGATCA T^GATCA
HaeII 207 AGCGCT [GA]GCGC^[CT]
Hin1I 2496 GACGTC G[GA]^CG[CT]C
HindIII 5079 AAGCTT A^AGCTT
MluNI 5324 TGGCCA TGG^CCA
MscI 5324 TGGCCA TGG^CCA
MvnI 1854 CGCG CG^CG
NdeI 5878 CATATG CA^TATG
NheI 3151 GCTAGC G^CTAGC
PmaCI 2725 CACGTG CAC^GTG
PmeI 2869 GTTTAAAC GTTT^AAAC
PmlI 2725 CACGTG CAC^GTG
PvuI 1907 CGATCG CGAT^CG
SacI 2365 GAGCTC GAGCT^C
ScaI 2614 AGTACT AGT^ACT
SexAI 4090 ACCTGGT A^CC[AT]GGT
SphI 5312 GCATGC GCATG^C
StuI 3786 AGGCCT AGG^CCT
XbaI 2776 TCTAGA T^CTAGA
XcmI 3498 CCAGTCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 1284 GTGCAC G^TGCAC
3478 GTGCAC
AocI 3670 CCTGAGG CC^T[ACGT]AGG
5727 CCTGAGG
ApaLI 1284 GTGCAC G^TGCAC
3478 GTGCAC
AspI 194 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1056 GACCATGTC
BglII 2005 AGATCT A^GATCT
4291 AGATCT
BsaBI 131 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1251 GATGCTAATC
BsrBI 1795 GAGCGG CCGCTC(-3/-3)
3257 GAGCGG
BssSI 5614 CACGAG CACGAG(-5/-1)
6108 CTCGTG
Bsu36I 3670 CCTGAGG CC^T[ACGT]AGG
5727 CCTGAGG
DrdI 1744 GACAAGTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4055 GACCCCAGTGTC
Eco81I 3670 CCTGAGG CC^T[ACGT]AGG
5727 CCTGAGG
MamI 131 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1251 GATGCTAATC
PacI 5489 TTAATTAA TTAAT^TAA
6481 TTAATTAA
PaeR7I 3463 CTCGAG C^TCGAG
4552 CTCGAG
PflFI 194 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1056 GACCATGTC
SpeI 2611 ACTAGT A^CTAGT
2917 ACTAGT
SwaI 4706 ATTTAAAT ATTT^AAAT
4742 ATTTAAAT
Tth111I 194 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1056 GACCATGTC
XhoI 3463 CTCGAG C^TCGAG
4552 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AccIII AciI AcsI AfaI
AflII AflIII AluI AlwI AlwNI
ApoI AseI AsnI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BfrI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BsaWI BseAI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspEI BspHI
BspLU11I BsrGI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoO65I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MroI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PshBI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI