##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI ApaI AscI AseI
AsnI Asp718I AviII AvrII BbeI
BcgI BlnI BsaHI BsaWI BseAI
BsiWI BspDI BspEI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraIII
EagI EclXI Eco52I FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 5040 CGCG CG^CG
Alw44I 2992 GTGCAC G^TGCAC
AocI 4002 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3488 AGCGCT AGC^GCT
ApaLI 2992 GTGCAC G^TGCAC
Asp700I 5468 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 4439 GGATCC G^GATCC
BbrPI 2138 CACGTG CAC^GTG
BciVI 4464 GGATAC GTATCC(6/5)
BclI 38 TGATCA T^GATCA
BsaAI 2138 CACGTG [CT]AC^GT[GA]
BsiEI 2531 CGACCG CG[GA][CT]^CG
BsmI 45 GAATGC GAATGC(1/-1)
Bsp1407I 19 TGTACA T^GTACA
BspHI 3919 TCATGA T^CATGA
BsrGI 19 TGTACA T^GTACA
BssSI 4877 CTCGTG CACGAG(-5/-1)
BstUI 5040 CGCG CG^CG
BstXI 176 CCAAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4002 CCTCAGG CC^T[ACGT]AGG
DraI 3194 TTTAAA TTT^AAA
Eco47III 3488 AGCGCT AGC^GCT
Eco81I 4002 CCTCAGG CC^T[ACGT]AGG
EcoRV 123 GATATC GAT^ATC
EcoT22I 1206 ATGCAT ATGCA^T
FbaI 38 TGATCA T^GATCA
HincII 69 GTCAAC GT[CT]^[GA]AC
HindII 69 GTCAAC GT[CT]^[GA]AC
MfeI 2079 CAATTG C^AATTG
MunI 2079 CAATTG C^AATTG
MvnI 5040 CGCG CG^CG
NdeI 4469 CATATG CA^TATG
NheI 5254 GCTAGC G^CTAGC
NsiI 1206 ATGCAT ATGCA^T
PflMI 108 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2138 CACGTG CAC^GTG
PmlI 2138 CACGTG CAC^GTG
RcaI 3919 TCATGA T^CATGA
SacI 711 GAGCTC GAGCT^C
SbfI 1184 CCTGCAGG CCTGCA^GG
ScaI 2660 AGTACT AGT^ACT
SphI 1508 GCATGC GCATG^C
Sse8387I 1184 CCTGCAGG CCTGCA^GG
SspBI 19 TGTACA T^GTACA
SspI 5382 AATATT AAT^ATT
Van91I 108 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2815 TCTAGA T^CTAGA
XmnI 5468 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspI 63 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2645 GACACTGTC
AvaI 4148 CCCGAG C^[CT]CG[GA]G
4773 CCCGAG
BalI 1282 TGGCCA TGG^CCA
4482 TGGCCA
BanII 711 GAGCTC G[GA]GC[CT]^C
1255 GAGCCC
BglI 1542 GCCAGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1872 GCCAGACTGGC
BsaBI 2439 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4107 GATGAGCATC
BsoBI 4148 CCCGAG C^[CT]CG[GA]G
4773 CCCGAG
BsrBI 1689 GAGCGG CCGCTC(-3/-3)
2403 GAGCGG
BsrFI 2344 ACCGGC [GA]^CCGG[CT]
5010 GCCGGT
BstEII 2868 GGTTACC G^GT[ACGT]ACC
4038 GGTTACC
BstPI 2868 GGTTACC G^GT[ACGT]ACC
4038 GGTTACC
Cfr10I 2344 ACCGGC [GA]^CCGG[CT]
5010 GCCGGT
DrdI 836 GACTTTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2802 GACTCTGCAGTC
EcoNI 1910 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3102 CCTTCTCAAGG
EcoO65I 2868 GGTTACC G^GT[ACGT]ACC
4038 GGTTACC
EcoRI 887 GAATTC G^AATTC
4664 GAATTC
HgaI 5038 GACGC GACGC(5/10)
67 GCGTC
MamI 2439 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4107 GATGAGCATC
MluNI 1282 TGGCCA TGG^CCA
4482 TGGCCA
MscI 1282 TGGCCA TGG^CCA
4482 TGGCCA
PflFI 63 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2645 GACACTGTC
SexAI 814 ACCTGGT A^CC[AT]GGT
4034 ACCAGGT
Tth111I 63 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2645 GACACTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AhdI AluI AlwI AlwNI ApoI
AspEI AspHI AvaII BanI BbsI
BbvCI BbvI BcnI BfaI BfrI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BspLU11I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII