################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII AclI Alw44I ApaLI AscI AseI AsnI BamHI BbeI BcgI BglII BlpI Bpu1102I BsgI BsiEI BsiWI BspDI BspHI BspLU11I BssHII Bst1107I BstBI BstZ17I CelII ClaI CpoI EagI EclXI Eco52I EcoRV EcoT22I FseI HindIII HpaI KasI KspI MfeI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 3136 GGTACC G^GTACC AccII 4147 CGCG CG^CG AccIII 155 TCCGGA T^CCGGA AcyI 3464 GGCGTC G[GA]^CG[CT]C AflII 1140 CTTAAG C^TTAAG AgeI 476 ACCGGT A^CCGGT AocI 421 CCTCAGG CC^T[ACGT]AGG Asp700I 3278 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 3136 GGTACC G^GTACC AspI 2113 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1805 TGCGCA TGC^GCA AvrII 4017 CCTAGG C^CTAGG BbrPI 1635 CACGTG CAC^GTG BfrI 1140 CTTAAG C^TTAAG BlnI 4017 CCTAGG C^CTAGG BsaAI 1635 CACGTG [CT]AC^GT[GA] BsaHI 3464 GGCGTC G[GA]^CG[CT]C BseAI 155 TCCGGA T^CCGGA BsmBI 1368 GAGACG CGTCTC(1/5) BspEI 155 TCCGGA T^CCGGA BsrDI 2071 CATTGC GCAATG(2/0) BssSI 3569 CACGAG CACGAG(-5/-1) BstAPI 636 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 4147 CGCG CG^CG Bsu36I 421 CCTCAGG CC^T[ACGT]AGG DrdI 1194 GACACAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 421 CCTCAGG CC^T[ACGT]AGG EcoNI 1816 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 1805 TGCGCA TGC^GCA Hin1I 3464 GGCGTC G[GA]^CG[CT]C KpnI 3136 GGTACC GGTAC^C MluI 4146 ACGCGT A^CGCGT MroI 155 TCCGGA T^CCGGA MvnI 4147 CGCG CG^CG NaeI 859 GCCGGC GCC^GGC NcoI 52 CCATGG C^CATGG NgoMIV 859 GCCGGC G^CCGGC PflFI 2113 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2036 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 476 ACCGGT A^CCGGT PmaCI 1635 CACGTG CAC^GTG PmlI 1635 CACGTG CAC^GTG PstI 87 CTGCAG CTGCA^G SacI 81 GAGCTC GAGCT^C SalI 1515 GTCGAC G^TCGAC SbfI 86 CCTGCAGG CCTGCA^GG SexAI 4176 ACCAGGT A^CC[AT]GGT Sse8387I 86 CCTGCAGG CCTGCA^GG StuI 1307 AGGCCT AGG^CCT Tth111I 2113 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2036 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 3278 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AflIII 1522 ACGTGT A^C[GA][CT]GT 4146 ACGCGT AlwNI 586 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG 2494 CAGCTACTG ApaI 2483 GGGCCC GGGCC^C 3525 GGGCCC AspHI 81 GAGCTC G[AT]GC[AT]^C 3072 GAGCAC BbvCI 409 CCTCAGC CCTCAGC(-5/-2) 2196 GCTGAGG BciVI 1061 GGATAC GTATCC(6/5) 945 GTATCC BclI 1387 TGATCA T^GATCA 2228 TGATCA BglI 1534 GCCCCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2768 GCCTACCTGGC BsiHKAI 81 GAGCTC G[AT]GC[AT]^C 3072 GAGCAC Bsp1407I 2528 TGTACA T^GTACA 3926 TGTACA BsrGI 2528 TGTACA T^GTACA 3926 TGTACA BstEII 618 GGTCACC G^GT[ACGT]ACC 2612 GGTGACC BstPI 618 GGTCACC G^GT[ACGT]ACC 2612 GGTGACC BstYI 540 AGATCC [GA]^GATC[CT] 3557 GGATCT DraI 4048 TTTAAA TTT^AAA 4455 TTTAAA EcoO65I 618 GGTCACC G^GT[ACGT]ACC 2612 GGTGACC EcoRI 1989 GAATTC G^AATTC 3174 GAATTC FbaI 1387 TGATCA T^GATCA 2228 TGATCA HgaI 1595 GCGTC GACGC(5/10) 3465 GCGTC MflI 540 AGATCC [GA]^GATC[CT] 3557 GGATCT SmaI 949 CCCGGG CCC^GGG 1710 CCCGGG SmlI 806 CTTGAG C^T[CT][GA]AG 1140 CTTAAG SspBI 2528 TGTACA T^GTACA 3926 TGTACA XbaI 938 TCTAGA T^CTAGA 3833 TCTAGA XhoII 540 AGATCC [GA]^GATC[CT] 3557 GGATCT XmaCI 949 CCCGGG C^CCGGG 1710 CCCGGG XmaI 949 CCCGGG C^CCGGG 1710 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccI AciI AcsI AfaI AhdI AluI AlwI Aor51HI ApoI AspEI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaBI BsaI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI Eam1105I EarI Eco47III EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI