##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI Alw44I ApaLI AscI
AseI AsnI BamHI BbeI BcgI
BglII BlpI Bpu1102I BsgI BsiEI
BsiWI BspDI BspHI BspLU11I BssHII
Bst1107I BstBI BstZ17I CelII ClaI
CpoI EagI EclXI Eco52I EcoRV
EcoT22I FseI HindIII HpaI KasI
KspI MfeI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 3136 GGTACC G^GTACC
AccII 4147 CGCG CG^CG
AccIII 155 TCCGGA T^CCGGA
AcyI 3464 GGCGTC G[GA]^CG[CT]C
AflII 1140 CTTAAG C^TTAAG
AgeI 476 ACCGGT A^CCGGT
AocI 421 CCTCAGG CC^T[ACGT]AGG
Asp700I 3278 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3136 GGTACC G^GTACC
AspI 2113 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1805 TGCGCA TGC^GCA
AvrII 4017 CCTAGG C^CTAGG
BbrPI 1635 CACGTG CAC^GTG
BfrI 1140 CTTAAG C^TTAAG
BlnI 4017 CCTAGG C^CTAGG
BsaAI 1635 CACGTG [CT]AC^GT[GA]
BsaHI 3464 GGCGTC G[GA]^CG[CT]C
BseAI 155 TCCGGA T^CCGGA
BsmBI 1368 GAGACG CGTCTC(1/5)
BspEI 155 TCCGGA T^CCGGA
BsrDI 2071 CATTGC GCAATG(2/0)
BssSI 3569 CACGAG CACGAG(-5/-1)
BstAPI 636 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 4147 CGCG CG^CG
Bsu36I 421 CCTCAGG CC^T[ACGT]AGG
DrdI 1194 GACACAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 421 CCTCAGG CC^T[ACGT]AGG
EcoNI 1816 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1805 TGCGCA TGC^GCA
Hin1I 3464 GGCGTC G[GA]^CG[CT]C
KpnI 3136 GGTACC GGTAC^C
MluI 4146 ACGCGT A^CGCGT
MroI 155 TCCGGA T^CCGGA
MvnI 4147 CGCG CG^CG
NaeI 859 GCCGGC GCC^GGC
NcoI 52 CCATGG C^CATGG
NgoMIV 859 GCCGGC G^CCGGC
PflFI 2113 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2036 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 476 ACCGGT A^CCGGT
PmaCI 1635 CACGTG CAC^GTG
PmlI 1635 CACGTG CAC^GTG
PstI 87 CTGCAG CTGCA^G
SacI 81 GAGCTC GAGCT^C
SalI 1515 GTCGAC G^TCGAC
SbfI 86 CCTGCAGG CCTGCA^GG
SexAI 4176 ACCAGGT A^CC[AT]GGT
Sse8387I 86 CCTGCAGG CCTGCA^GG
StuI 1307 AGGCCT AGG^CCT
Tth111I 2113 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2036 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3278 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 1522 ACGTGT A^C[GA][CT]GT
4146 ACGCGT
AlwNI 586 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
2494 CAGCTACTG
ApaI 2483 GGGCCC GGGCC^C
3525 GGGCCC
AspHI 81 GAGCTC G[AT]GC[AT]^C
3072 GAGCAC
BbvCI 409 CCTCAGC CCTCAGC(-5/-2)
2196 GCTGAGG
BciVI 1061 GGATAC GTATCC(6/5)
945 GTATCC
BclI 1387 TGATCA T^GATCA
2228 TGATCA
BglI 1534 GCCCCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2768 GCCTACCTGGC
BsiHKAI 81 GAGCTC G[AT]GC[AT]^C
3072 GAGCAC
Bsp1407I 2528 TGTACA T^GTACA
3926 TGTACA
BsrGI 2528 TGTACA T^GTACA
3926 TGTACA
BstEII 618 GGTCACC G^GT[ACGT]ACC
2612 GGTGACC
BstPI 618 GGTCACC G^GT[ACGT]ACC
2612 GGTGACC
BstYI 540 AGATCC [GA]^GATC[CT]
3557 GGATCT
DraI 4048 TTTAAA TTT^AAA
4455 TTTAAA
EcoO65I 618 GGTCACC G^GT[ACGT]ACC
2612 GGTGACC
EcoRI 1989 GAATTC G^AATTC
3174 GAATTC
FbaI 1387 TGATCA T^GATCA
2228 TGATCA
HgaI 1595 GCGTC GACGC(5/10)
3465 GCGTC
MflI 540 AGATCC [GA]^GATC[CT]
3557 GGATCT
SmaI 949 CCCGGG CCC^GGG
1710 CCCGGG
SmlI 806 CTTGAG C^T[CT][GA]AG
1140 CTTAAG
SspBI 2528 TGTACA T^GTACA
3926 TGTACA
XbaI 938 TCTAGA T^CTAGA
3833 TCTAGA
XhoII 540 AGATCC [GA]^GATC[CT]
3557 GGATCT
XmaCI 949 CCCGGG C^CCGGG
1710 CCCGGG
XmaI 949 CCCGGG C^CCGGG
1710 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AciI AcsI AfaI AhdI
AluI AlwI Aor51HI ApoI AspEI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaBI BsaI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI Eam1105I EarI Eco47III
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI