##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AatII       AclI        Alw44I      ApaLI       AscI        
AseI        AsnI        BamHI       BbeI        BcgI        
BglII       BlpI        Bpu1102I    BsgI        BsiEI       
BsiWI       BspDI       BspHI       BspLU11I    BssHII      
Bst1107I    BstBI       BstZ17I     CelII       ClaI        
CpoI        EagI        EclXI       Eco52I      EcoRV       
EcoT22I     FseI        HindIII     HpaI        KasI        
KspI        MfeI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
ScaI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        SspI        SwaI        
XhoI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I       3136  GGTACC               G^GTACC
AccII        4147  CGCG                 CG^CG
AccIII        155  TCCGGA               T^CCGGA
AcyI         3464  GGCGTC               G[GA]^CG[CT]C
AflII        1140  CTTAAG               C^TTAAG
AgeI          476  ACCGGT               A^CCGGT
AocI          421  CCTCAGG              CC^T[ACGT]AGG
Asp700I      3278  GAACACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      3136  GGTACC               G^GTACC
AspI         2113  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1805  TGCGCA               TGC^GCA
AvrII        4017  CCTAGG               C^CTAGG
BbrPI        1635  CACGTG               CAC^GTG
BfrI         1140  CTTAAG               C^TTAAG
BlnI         4017  CCTAGG               C^CTAGG
BsaAI        1635  CACGTG               [CT]AC^GT[GA]
BsaHI        3464  GGCGTC               G[GA]^CG[CT]C
BseAI         155  TCCGGA               T^CCGGA
BsmBI        1368  GAGACG               CGTCTC(1/5)
BspEI         155  TCCGGA               T^CCGGA
BsrDI        2071  CATTGC               GCAATG(2/0)
BssSI        3569  CACGAG               CACGAG(-5/-1)
BstAPI        636  GCAGCAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        4147  CGCG                 CG^CG
Bsu36I        421  CCTCAGG              CC^T[ACGT]AGG
DrdI         1194  GACACAAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        421  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1816  CCTGGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI         1805  TGCGCA               TGC^GCA
Hin1I        3464  GGCGTC               G[GA]^CG[CT]C
KpnI         3136  GGTACC               GGTAC^C
MluI         4146  ACGCGT               A^CGCGT
MroI          155  TCCGGA               T^CCGGA
MvnI         4147  CGCG                 CG^CG
NaeI          859  GCCGGC               GCC^GGC
NcoI           52  CCATGG               C^CATGG
NgoMIV        859  GCCGGC               G^CCGGC
PflFI        2113  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2036  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         476  ACCGGT               A^CCGGT
PmaCI        1635  CACGTG               CAC^GTG
PmlI         1635  CACGTG               CAC^GTG
PstI           87  CTGCAG               CTGCA^G
SacI           81  GAGCTC               GAGCT^C
SalI         1515  GTCGAC               G^TCGAC
SbfI           86  CCTGCAGG             CCTGCA^GG
SexAI        4176  ACCAGGT              A^CC[AT]GGT
Sse8387I       86  CCTGCAGG             CCTGCA^GG
StuI         1307  AGGCCT               AGG^CCT
Tth111I      2113  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2036  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         3278  GAACACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AflIII       1522  ACGTGT               A^C[GA][CT]GT
             4146  ACGCGT              
AlwNI         586  CAGAGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2494  CAGCTACTG           
ApaI         2483  GGGCCC               GGGCC^C
             3525  GGGCCC              
AspHI          81  GAGCTC               G[AT]GC[AT]^C
             3072  GAGCAC              
BbvCI         409  CCTCAGC              CCTCAGC(-5/-2)
             2196  GCTGAGG             
BciVI        1061  GGATAC               GTATCC(6/5)
              945  GTATCC              
BclI         1387  TGATCA               T^GATCA
             2228  TGATCA              
BglI         1534  GCCCCAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2768  GCCTACCTGGC         
BsiHKAI        81  GAGCTC               G[AT]GC[AT]^C
             3072  GAGCAC              
Bsp1407I     2528  TGTACA               T^GTACA
             3926  TGTACA              
BsrGI        2528  TGTACA               T^GTACA
             3926  TGTACA              
BstEII        618  GGTCACC              G^GT[ACGT]ACC
             2612  GGTGACC             
BstPI         618  GGTCACC              G^GT[ACGT]ACC
             2612  GGTGACC             
BstYI         540  AGATCC               [GA]^GATC[CT]
             3557  GGATCT              
DraI         4048  TTTAAA               TTT^AAA
             4455  TTTAAA              
EcoO65I       618  GGTCACC              G^GT[ACGT]ACC
             2612  GGTGACC             
EcoRI        1989  GAATTC               G^AATTC
             3174  GAATTC              
FbaI         1387  TGATCA               T^GATCA
             2228  TGATCA              
HgaI         1595  GCGTC                GACGC(5/10)
             3465  GCGTC               
MflI          540  AGATCC               [GA]^GATC[CT]
             3557  GGATCT              
SmaI          949  CCCGGG               CCC^GGG
             1710  CCCGGG              
SmlI          806  CTTGAG               C^T[CT][GA]AG
             1140  CTTAAG              
SspBI        2528  TGTACA               T^GTACA
             3926  TGTACA              
XbaI          938  TCTAGA               T^CTAGA
             3833  TCTAGA              
XhoII         540  AGATCC               [GA]^GATC[CT]
             3557  GGATCT              
XmaCI         949  CCCGGG               C^CCGGG
             1710  CCCGGG              
XmaI          949  CCCGGG               C^CCGGG
             1710  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AhdI        
AluI        AlwI        Aor51HI     ApoI        AspEI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaBI       BsaI        BsaJI       
BsaWI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstXI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        Eam1105I    EarI        Eco47III    
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI