##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AclI AscI AseI AsnI
BbeI BcgI BglI BsaBI BsiEI
BspDI BsrBI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraIII
DrdI EagI EclXI Eco52I EcoRV
FseI KasI KspI MamI MluI
NaeI NarI NgoMIV NotI NruI
NspV PacI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 387 GTAGAC GT^[AC][GT]AC
AccII 3510 CGCG CG^CG
AcyI 439 GGCGTC G[GA]^CG[CT]C
AgeI 354 ACCGGT A^CCGGT
AocI 485 CCTAAGG CC^T[ACGT]AGG
Asp700I 4236 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2554 TGCGCA TGC^GCA
AvrII 501 CCTAGG C^CTAGG
BbrPI 4089 CACGTG CAC^GTG
BciVI 408 GTATCC GTATCC(6/5)
BlnI 501 CCTAGG C^CTAGG
BlpI 160 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 160 GCTGAGC GC^T[ACGT]AGC
BsaAI 4089 CACGTG [CT]AC^GT[GA]
BsaHI 439 GGCGTC G[GA]^CG[CT]C
BsiWI 2819 CGTACG C^GTACG
BspHI 1328 TCATGA T^CATGA
BspLU11I 1888 ACATGT A^CATGT
BsrFI 354 ACCGGT [GA]^CCGG[CT]
BstEII 2483 GGTAACC G^GT[ACGT]ACC
BstPI 2483 GGTAACC G^GT[ACGT]ACC
BstUI 3510 CGCG CG^CG
Bsu36I 485 CCTAAGG CC^T[ACGT]AGG
CelII 160 GCTGAGC GC^T[ACGT]AGC
Cfr10I 354 ACCGGT [GA]^CCGG[CT]
Eco81I 485 CCTAAGG CC^T[ACGT]AGG
EcoO65I 2483 GGTAACC G^GT[ACGT]ACC
EcoRI 656 GAATTC G^AATTC
FspI 2554 TGCGCA TGC^GCA
Hin1I 439 GGCGTC G[GA]^CG[CT]C
HindIII 2088 AAGCTT A^AGCTT
HpaI 1274 GTTAAC GTT^AAC
MfeI 1180 CAATTG C^AATTG
MunI 1180 CAATTG C^AATTG
MvnI 3510 CGCG CG^CG
NdeI 1361 CATATG CA^TATG
NheI 4186 GCTAGC G^CTAGC
PaeR7I 3250 CTCGAG C^TCGAG
PinAI 354 ACCGGT A^CCGGT
PmaCI 4089 CACGTG CAC^GTG
PmlI 4089 CACGTG CAC^GTG
PshAI 1127 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1328 TCATGA T^CATGA
SacI 2500 GAGCTC GAGCT^C
SapI 448 GAAGAGC GCTCTTC(1/4)
ScaI 2470 AGTACT AGT^ACT
XhoI 3250 CTCGAG C^TCGAG
XmnI 4236 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 3788 GGTACC G^GTACC
4374 GGTACC
AccIII 1299 TCCGGA T^CCGGA
3441 TCCGGA
AflIII 1270 ACACGT A^C[GA][CT]GT
1888 ACATGT
Asp718I 3788 GGTACC G^GTACC
4374 GGTACC
BclI 853 TGATCA T^GATCA
1667 TGATCA
BseAI 1299 TCCGGA T^CCGGA
3441 TCCGGA
BsmBI 2022 CGTCTC CGTCTC(1/5)
2837 CGTCTC
BsmI 2903 GAATGC GAATGC(1/-1)
2509 GCATTC
BspEI 1299 TCCGGA T^CCGGA
3441 TCCGGA
DraI 4556 TTTAAA TTT^AAA
4699 TTTAAA
EcoT22I 2905 ATGCAT ATGCA^T
3950 ATGCAT
FbaI 853 TGATCA T^GATCA
1667 TGATCA
HincII 1083 GTTGAC GT[CT]^[GA]AC
1274 GTTAAC
HindII 1083 GTTGAC GT[CT]^[GA]AC
1274 GTTAAC
KpnI 3788 GGTACC GGTAC^C
4374 GGTACC
MaeII 1272 ACGT A^CGT
4090 ACGT
MroI 1299 TCCGGA T^CCGGA
3441 TCCGGA
NsiI 2905 ATGCAT ATGCA^T
3950 ATGCAT
PflMI 327 CCATTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2043 CCATCTCATGG
SexAI 367 ACCTGGT A^CC[AT]GGT
3784 ACCTGGT
StuI 1807 AGGCCT AGG^CCT
2942 AGGCCT
Van91I 327 CCATTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2043 CCATCTCATGG
XbaI 152 TCTAGA T^CTAGA
4669 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AciI AcsI AfaI AflII AhdI
AluI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI ApoI AspEI AspHI
AspI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BfrI BglII BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eam1105I EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII