################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI BbeI BbrPI BbvCI BcgI BfrI BglII BlpI Bpu1102I BsaWI BseAI BsiWI BspEI Bst1107I BstZ17I CelII CpoI Eam1105I Eco47III EcoNI FseI HgaI KasI MluI MroI NarI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SbfI SfiI SfoI SgrAI SnaBI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 1390 GACGTC GACGT^C Acc65I 3421 GGTACC G^GTACC AcyI 1390 GACGTC G[GA]^CG[CT]C ApaI 718 GGGCCC GGGCC^C Asp718I 3421 GGTACC G^GTACC AviII 4016 TGCGCA TGC^GCA AvrII 111 CCTAGG C^CTAGG BciVI 933 GGATAC GTATCC(6/5) BclI 5020 TGATCA T^GATCA BlnI 111 CCTAGG C^CTAGG BsaHI 1390 GACGTC G[GA]^CG[CT]C BsmBI 560 GAGACG CGTCTC(1/5) BspDI 4498 ATCGAT AT^CGAT BsrDI 5232 CATTGC GCAATG(2/0) BssHII 124 GCGCGC G^CGCGC BssSI 4899 CTCGTG CACGAG(-5/-1) BstEII 4810 GGTCACC G^GT[ACGT]ACC BstPI 4810 GGTCACC G^GT[ACGT]ACC BstXI 5151 CCAATTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 4498 ATCGAT AT^CGAT DraIII 2911 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4897 GACTCGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 4810 GGTCACC G^GT[ACGT]ACC EcoRV 4501 GATATC GAT^ATC FbaI 5020 TGATCA T^GATCA FspI 4016 TGCGCA TGC^GCA HaeII 545 AGCGCC [GA]GCGC^[CT] Hin1I 1390 GACGTC G[GA]^CG[CT]C HindIII 1249 AAGCTT A^AGCTT KpnI 3421 GGTACC GGTAC^C KspI 627 CCGCGG CCGC^GG MfeI 2228 CAATTG C^AATTG MunI 2228 CAATTG C^AATTG NcoI 1916 CCATGG C^CATGG PvuII 5951 CAGCTG CAG^CTG SacI 5210 GAGCTC GAGCT^C SacII 627 CCGCGG CCGC^GG SalI 1148 GTCGAC G^TCGAC ScaI 3608 AGTACT AGT^ACT SpeI 5394 ACTAGT A^CTAGT XcmI 2235 CCATTCTGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AocI 374 CCTGAGG CC^T[ACGT]AGG 3128 CCTTAGG Asp700I 1477 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1657 GAAAAATTTC BalI 239 TGGCCA TGG^CCA 807 TGGCCA BamHI 4631 GGATCC G^GATCC 4845 GGATCC BanI 3421 GGTACC G^G[CT][GA]CC 5777 GGCACC BpmI 4804 CTCCAG CTGGAG(16/14) 5215 CTCCAG BsaAI 826 TACGTG [CT]AC^GT[GA] 1433 CACGTA BsaBI 2398 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3810 GATACAGATC Bsp1407I 3191 TGTACA T^GTACA 4400 TGTACA BspHI 3324 TCATGA T^CATGA 3532 TCATGA BsrGI 3191 TGTACA T^GTACA 4400 TGTACA BstAPI 3938 GCACTGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4155 GCATTATGTGC BstBI 1576 TTCGAA TT^CGAA 3856 TTCGAA Bsu36I 374 CCTGAGG CC^T[ACGT]AGG 3128 CCTTAGG DsaI 627 CCGCGG C^C[GA][CT]GG 1916 CCATGG Eco81I 374 CCTGAGG CC^T[ACGT]AGG 3128 CCTTAGG EcoRI 2591 GAATTC G^AATTC 5623 GAATTC HpaI 1729 GTTAAC GTT^AAC 1974 GTTAAC MamI 2398 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3810 GATACAGATC MluNI 239 TGGCCA TGG^CCA 807 TGGCCA MscI 239 TGGCCA TGG^CCA 807 TGGCCA NaeI 51 GCCGGC GCC^GGC 727 GCCGGC NdeI 3472 CATATG CA^TATG 3798 CATATG NgoMIV 51 GCCGGC G^CCGGC 727 GCCGGC NheI 3065 GCTAGC G^CTAGC 3884 GCTAGC NotI 517 GCGGCCGC GC^GGCCGC 672 GCGGCCGC NspV 1576 TTCGAA TT^CGAA 3856 TTCGAA PflMI 3139 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3839 CCAACTTATGG RcaI 3324 TCATGA T^CATGA 3532 TCATGA SapI 218 GAAGAGC GCTCTTC(1/4) 2494 GCTCTTC SexAI 1070 ACCTGGT A^CC[AT]GGT 1116 ACCAGGT SfuI 1576 TTCGAA TT^CGAA 3856 TTCGAA SphI 4142 GCATGC GCATG^C 4474 GCATGC SspBI 3191 TGTACA T^GTACA 4400 TGTACA SspI 2005 AATATT AAT^ATT 5924 AATATT StuI 29 AGGCCT AGG^CCT 2963 AGGCCT Van91I 3139 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3839 CCAACTTATGG XbaI 888 TCTAGA T^CTAGA 5627 TCTAGA XmnI 1477 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1657 GAAAAATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AspI AvaI AvaII BanII BbsI BbvI BcnI BfaI BglI BmyI BpuAI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflFI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII XmaCI XmaI