##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI BbeI BbrPI BbvCI
BcgI BfrI BglII BlpI Bpu1102I
BsaWI BseAI BsiWI BspEI Bst1107I
BstZ17I CelII CpoI Eam1105I Eco47III
EcoNI FseI HgaI KasI MluI
MroI NarI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SbfI
SfiI SfoI SgrAI SnaBI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1390 GACGTC GACGT^C
Acc65I 3421 GGTACC G^GTACC
AcyI 1390 GACGTC G[GA]^CG[CT]C
ApaI 718 GGGCCC GGGCC^C
Asp718I 3421 GGTACC G^GTACC
AviII 4016 TGCGCA TGC^GCA
AvrII 111 CCTAGG C^CTAGG
BciVI 933 GGATAC GTATCC(6/5)
BclI 5020 TGATCA T^GATCA
BlnI 111 CCTAGG C^CTAGG
BsaHI 1390 GACGTC G[GA]^CG[CT]C
BsmBI 560 GAGACG CGTCTC(1/5)
BspDI 4498 ATCGAT AT^CGAT
BsrDI 5232 CATTGC GCAATG(2/0)
BssHII 124 GCGCGC G^CGCGC
BssSI 4899 CTCGTG CACGAG(-5/-1)
BstEII 4810 GGTCACC G^GT[ACGT]ACC
BstPI 4810 GGTCACC G^GT[ACGT]ACC
BstXI 5151 CCAATTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 4498 ATCGAT AT^CGAT
DraIII 2911 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4897 GACTCGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4810 GGTCACC G^GT[ACGT]ACC
EcoRV 4501 GATATC GAT^ATC
FbaI 5020 TGATCA T^GATCA
FspI 4016 TGCGCA TGC^GCA
HaeII 545 AGCGCC [GA]GCGC^[CT]
Hin1I 1390 GACGTC G[GA]^CG[CT]C
HindIII 1249 AAGCTT A^AGCTT
KpnI 3421 GGTACC GGTAC^C
KspI 627 CCGCGG CCGC^GG
MfeI 2228 CAATTG C^AATTG
MunI 2228 CAATTG C^AATTG
NcoI 1916 CCATGG C^CATGG
PvuII 5951 CAGCTG CAG^CTG
SacI 5210 GAGCTC GAGCT^C
SacII 627 CCGCGG CCGC^GG
SalI 1148 GTCGAC G^TCGAC
ScaI 3608 AGTACT AGT^ACT
SpeI 5394 ACTAGT A^CTAGT
XcmI 2235 CCATTCTGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AocI 374 CCTGAGG CC^T[ACGT]AGG
3128 CCTTAGG
Asp700I 1477 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1657 GAAAAATTTC
BalI 239 TGGCCA TGG^CCA
807 TGGCCA
BamHI 4631 GGATCC G^GATCC
4845 GGATCC
BanI 3421 GGTACC G^G[CT][GA]CC
5777 GGCACC
BpmI 4804 CTCCAG CTGGAG(16/14)
5215 CTCCAG
BsaAI 826 TACGTG [CT]AC^GT[GA]
1433 CACGTA
BsaBI 2398 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3810 GATACAGATC
Bsp1407I 3191 TGTACA T^GTACA
4400 TGTACA
BspHI 3324 TCATGA T^CATGA
3532 TCATGA
BsrGI 3191 TGTACA T^GTACA
4400 TGTACA
BstAPI 3938 GCACTGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4155 GCATTATGTGC
BstBI 1576 TTCGAA TT^CGAA
3856 TTCGAA
Bsu36I 374 CCTGAGG CC^T[ACGT]AGG
3128 CCTTAGG
DsaI 627 CCGCGG C^C[GA][CT]GG
1916 CCATGG
Eco81I 374 CCTGAGG CC^T[ACGT]AGG
3128 CCTTAGG
EcoRI 2591 GAATTC G^AATTC
5623 GAATTC
HpaI 1729 GTTAAC GTT^AAC
1974 GTTAAC
MamI 2398 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3810 GATACAGATC
MluNI 239 TGGCCA TGG^CCA
807 TGGCCA
MscI 239 TGGCCA TGG^CCA
807 TGGCCA
NaeI 51 GCCGGC GCC^GGC
727 GCCGGC
NdeI 3472 CATATG CA^TATG
3798 CATATG
NgoMIV 51 GCCGGC G^CCGGC
727 GCCGGC
NheI 3065 GCTAGC G^CTAGC
3884 GCTAGC
NotI 517 GCGGCCGC GC^GGCCGC
672 GCGGCCGC
NspV 1576 TTCGAA TT^CGAA
3856 TTCGAA
PflMI 3139 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3839 CCAACTTATGG
RcaI 3324 TCATGA T^CATGA
3532 TCATGA
SapI 218 GAAGAGC GCTCTTC(1/4)
2494 GCTCTTC
SexAI 1070 ACCTGGT A^CC[AT]GGT
1116 ACCAGGT
SfuI 1576 TTCGAA TT^CGAA
3856 TTCGAA
SphI 4142 GCATGC GCATG^C
4474 GCATGC
SspBI 3191 TGTACA T^GTACA
4400 TGTACA
SspI 2005 AATATT AAT^ATT
5924 AATATT
StuI 29 AGGCCT AGG^CCT
2963 AGGCCT
Van91I 3139 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3839 CCAACTTATGG
XbaI 888 TCTAGA T^CTAGA
5627 TCTAGA
XmnI 1477 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1657 GAAAAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AspI AvaI AvaII BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpuAI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PflFI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII XmaCI
XmaI