##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI AscI Asp718I AspEI AspI
AvrII BbeI BbvCI BcgI BciVI
BcnI BlnI BlpI Bpu1102I BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BsrBI BssHII BssSI
BstBI CelII ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoRI
FseI Hin1I KasI KpnI KspI
MluI MroI NarI NciI NcoI
NheI NotI NruI NspV PaeR7I
PflFI PinAI PmeI PpuMI PvuI
RsrII SacII SalI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Tth111I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AclI 7032 AACGTT AA^CGTT
AflII 707 CTTAAG C^TTAAG
Alw44I 6390 GTGCAC G^TGCAC
Aor51HI 3025 AGCGCT AGC^GCT
ApaI 3778 GGGCCC GGGCC^C
ApaLI 6390 GTGCAC G^TGCAC
AvaI 6032 CCCGAG C^[CT]CG[GA]G
AviII 2869 TGCGCA TGC^GCA
BanI 4850 GGTGCC G^G[CT][GA]CC
BbrPI 7116 CACGTG CAC^GTG
BfrI 707 CTTAAG C^TTAAG
BglI 4875 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 7587 AGATCT A^GATCT
BsaBI 553 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 4929 CGTCTC CGTCTC(1/5)
BsoBI 6032 CCCGAG C^[CT]CG[GA]G
BsrFI 3795 GCCGGC [GA]^CCGG[CT]
Bst1107I 2757 GTATAC GTA^TAC
BstXI 3244 CCAAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2757 GTATAC GTA^TAC
Cfr10I 3795 GCCGGC [GA]^CCGG[CT]
Eco47III 3025 AGCGCT AGC^GCT
EcoNI 1772 CCTCTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1568 GCGGG CCCGC(4/6)
FspI 2869 TGCGCA TGC^GCA
HaeII 3025 AGCGCT [GA]GCGC^[CT]
HapII 3796 CCGG C^CGG
HpaI 6378 GTTAAC GTT^AAC
HpaII 3796 CCGG C^CGG
MamI 553 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 7081 CAATTG C^AATTG
MspI 3796 CCGG C^CGG
MunI 7081 CAATTG C^AATTG
NaeI 3795 GCCGGC GCC^GGC
NgoMIV 3795 GCCGGC G^CCGGC
PacI 4424 TTAATTAA TTAAT^TAA
PmaCI 7116 CACGTG CAC^GTG
PmlI 7116 CACGTG CAC^GTG
PshAI 2065 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 7032 AACGTT AA^CGTT
SbfI 4877 CCTGCAGG CCTGCA^GG
ScaI 6075 AGTACT AGT^ACT
SphI 5934 GCATGC GCATG^C
Sse8387I 4877 CCTGCAGG CCTGCA^GG
StuI 6898 AGGCCT AGG^CCT
TaqI 1998 TCGA T^CGA
TthHB8I 1998 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AocI 6894 CCTCAGG CC^T[ACGT]AGG
7534 CCTTAGG
BamHI 280 GGATCC G^GATCC
5637 GGATCC
BclI 271 TGATCA T^GATCA
307 TGATCA
Bsp1407I 2501 TGTACA T^GTACA
6511 TGTACA
BspLU11I 4296 ACATGT A^CATGT
6948 ACATGT
BspMI 4871 ACCTGC ACCTGC(4/8)
4107 GCAGGT
BsrGI 2501 TGTACA T^GTACA
6511 TGTACA
Bsu36I 6894 CCTCAGG CC^T[ACGT]AGG
7534 CCTTAGG
DraII 3777 GGGGCCC [GA]G^G[ACGT]CC[CT]
3778 GGGCCCT
DraIII 5346 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
5543 CACCGAGTG
Eco81I 6894 CCTCAGG CC^T[ACGT]AGG
7534 CCTTAGG
EcoO109I 3777 GGGGCCC [GA]G^G[ACGT]CC[CT]
3778 GGGCCCT
EcoRV 7373 GATATC GAT^ATC
7678 GATATC
EcoT14I 761 CCAAGG C^C[AT][AT]GG
5013 CCTTGG
FbaI 271 TGATCA T^GATCA
307 TGATCA
HgaI 7121 GACGC GACGC(5/10)
2989 GCGTC
MspA1I 49 CAGCTG C[AC]G^C[GT]G
2189 CAGCTG
NdeI 10 CATATG CA^TATG
1935 CATATG
PvuII 49 CAGCTG CAG^CTG
2189 CAGCTG
SacI 2277 GAGCTC GAGCT^C
6184 GAGCTC
SspBI 2501 TGTACA T^GTACA
6511 TGTACA
StyI 761 CCAAGG C^C[AT][AT]GG
5013 CCTTGG
SwaI 512 ATTTAAAT ATTT^AAAT
3508 ATTTAAAT
XbaI 5830 TCTAGA T^CTAGA
7369 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AspHI AvaII
BalI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BsrDI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoO65I
EcoRII EcoT22I Fnu4HI FokI HaeIII
HhaI HinP1I HincII HindII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PshBI PstI RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII
XmnI