##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AscI Asp718I BbeI BsiEI BsiWI
BsmBI BspDI BspHI BssHII ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I FseI HaeII KasI KpnI
KspI MluI NarI NdeI NotI
NruI PacI PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SexAI SfiI SfoI SgrAI
SnaBI SphI SspI SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcyI 63 GGCGTC G[GA]^CG[CT]C
AflII 6378 CTTAAG C^TTAAG
AflIII 3670 ACATGT A^C[GA][CT]GT
AhdI 4629 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5361 GTGCAC G^TGCAC
ApaLI 5361 GTGCAC G^TGCAC
AspEI 4629 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2677 TGCGCA TGC^GCA
AvrII 5581 CCTAGG C^CTAGG
BbrPI 4443 CACGTG CAC^GTG
BcgI 3957 CGAGATAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2143 GGATAC GTATCC(6/5)
BfrI 6378 CTTAAG C^TTAAG
BlnI 5581 CCTAGG C^CTAGG
BlpI 2753 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2753 GCTGAGC GC^T[ACGT]AGC
BsaAI 4443 CACGTG [CT]AC^GT[GA]
BsaBI 2039 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 63 GGCGTC G[GA]^CG[CT]C
BspLU11I 3670 ACATGT A^CATGT
BsrDI 3803 GCAATG GCAATG(2/0)
Bst1107I 4903 GTATAC GTA^TAC
BstEII 4106 GGTAACC G^GT[ACGT]ACC
BstPI 4106 GGTAACC G^GT[ACGT]ACC
BstZ17I 4903 GTATAC GTA^TAC
CelII 2753 GCTGAGC GC^T[ACGT]AGC
Eam1105I 4629 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4106 GGTAACC G^GT[ACGT]ACC
EcoT22I 3937 ATGCAT ATGCA^T
FspI 2677 TGCGCA TGC^GCA
Hin1I 63 GGCGTC G[GA]^CG[CT]C
HpaI 5729 GTTAAC GTT^AAC
MamI 2039 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 7169 CAATTG C^AATTG
MunI 7169 CAATTG C^AATTG
NaeI 3383 GCCGGC GCC^GGC
NgoMIV 3383 GCCGGC G^CCGGC
NheI 5280 GCTAGC G^CTAGC
NsiI 3937 ATGCAT ATGCA^T
PaeR7I 4252 CTCGAG C^TCGAG
PmaCI 4443 CACGTG CAC^GTG
PmlI 4443 CACGTG CAC^GTG
SbfI 1069 CCTGCAGG CCTGCA^GG
ScaI 5823 AGTACT AGT^ACT
SmaI 2013 CCCGGG CCC^GGG
SpeI 4691 ACTAGT A^CTAGT
Sse8387I 1069 CCTGCAGG CCTGCA^GG
XhoI 4252 CTCGAG C^TCGAG
XmaCI 2013 CCCGGG C^CCGGG
XmaI 2013 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 4046 GTAGAC GT^[AC][GT]AC
4903 GTATAC
AccII 1226 CGCG CG^CG
1964 CGCG
AseI 4141 ATTAAT AT^TAAT
6962 ATTAAT
AsnI 4141 ATTAAT AT^TAAT
6962 ATTAAT
AspI 2161 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2842 GACTTTGTC
BalI 1713 TGGCCA TGG^CCA
3236 TGGCCA
BamHI 1772 GGATCC G^GATCC
3531 GGATCC
BbvCI 3659 CCTCAGC CCTCAGC(-5/-2)
2456 GCTGAGG
BglI 3817 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
7224 GCCTGTCAGGC
Bsp1407I 3195 TGTACA T^GTACA
7266 TGTACA
BsrBI 1292 GAGCGG CCGCTC(-3/-3)
4872 GAGCGG
BsrFI 1904 GCCGGT [GA]^CCGG[CT]
3383 GCCGGC
BsrGI 3195 TGTACA T^GTACA
7266 TGTACA
BstAPI 1486 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1531 GCAACAGCTGC
BstUI 1226 CGCG CG^CG
1964 CGCG
Cfr10I 1904 GCCGGT [GA]^CCGG[CT]
3383 GCCGGC
EaeI 1713 TGGCCA [CT]^GGCC[GA]
3236 TGGCCA
EcoNI 282 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2717 CCTGAAGGAGG
EcoRI 1696 GAATTC G^AATTC
7100 GAATTC
EcoRV 4004 GATATC GAT^ATC
6013 GATATC
FauI 1965 GCGGG CCCGC(4/6)
3967 GCGGG
MaeII 1571 ACGT A^CGT
4444 ACGT
MluNI 1713 TGGCCA TGG^CCA
3236 TGGCCA
MscI 1713 TGGCCA TGG^CCA
3236 TGGCCA
MvnI 1226 CGCG CG^CG
1964 CGCG
PflFI 2161 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2842 GACTTTGTC
PshBI 4141 ATTAAT AT^TAAT
6962 ATTAAT
SapI 133 GAAGAGC GCTCTTC(1/4)
6310 GAAGAGC
SspBI 3195 TGTACA T^GTACA
7266 TGTACA
StuI 279 AGGCCT AGG^CCT
2714 AGGCCT
Tth111I 2161 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2842 GACTTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccIII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvI BclI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspEI
BspMI BsrI BssKI BssSI BstBI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EarI
Eco81I EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MroI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI NspV
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfuI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII XmnI