##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AclI AflII AgeI Aor51HI
AscI Asp718I AspI AvaI BalI
BamHI BanI BbeI BbrPI BclI
BcnI BfrI BglI BsaBI BsiEI
BsiWI BsoBI Bsp1407I BsrGI BssHII
Bst1107I BstBI BstZ17I CpoI EagI
EclXI Eco47III Eco52I FbaI FseI
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI NaeI NarI NciI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SexAI SfiI SfoI SfuI
SmaI SnaBI SspBI SspI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1604 GACGTC GACGT^C
AccII 4105 CGCG CG^CG
AccIII 6059 TCCGGA T^CCGGA
AflIII 3895 ACATGT A^C[GA][CT]GT
AhdI 2981 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2981 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3270 TGCGCA TGC^GCA
AvrII 3155 CCTAGG C^CTAGG
BbvCI 5043 GCTGAGG CCTCAGC(-5/-2)
BciVI 4841 GGATAC GTATCC(6/5)
BglII 2438 AGATCT A^GATCT
BlnI 3155 CCTAGG C^CTAGG
BsaWI 6059 TCCGGA [AT]^CCGG[AT]
BseAI 6059 TCCGGA T^CCGGA
BspDI 4648 ATCGAT AT^CGAT
BspEI 6059 TCCGGA T^CCGGA
BspLU11I 3895 ACATGT A^CATGT
BsrFI 1207 GCCGGT [GA]^CCGG[CT]
BstEII 3034 GGTGACC G^GT[ACGT]ACC
BstPI 3034 GGTGACC G^GT[ACGT]ACC
BstUI 4105 CGCG CG^CG
BstXI 5867 CCACTATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1207 GCCGGT [GA]^CCGG[CT]
ClaI 4648 ATCGAT AT^CGAT
DraIII 3935 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 5229 CGGCCA [CT]^GGCC[GA]
Eam1105I 2981 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3034 GGTGACC G^GT[ACGT]ACC
EcoRV 5585 GATATC GAT^ATC
FspI 3270 TGCGCA TGC^GCA
MroI 6059 TCCGGA T^CCGGA
MvnI 4105 CGCG CG^CG
SbfI 482 CCTGCAGG CCTGCA^GG
ScaI 4135 AGTACT AGT^ACT
SgrAI 1206 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 482 CCTGCAGG CCTGCA^GG
StuI 1817 AGGCCT AGG^CCT
XcmI 1185 CCAGACAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 1604 GACGTC G[GA]^CG[CT]C
2968 GGCGTC
Alw44I 1623 GTGCAC G^TGCAC
3874 GTGCAC
AocI 4836 CCTAAGG CC^T[ACGT]AGG
5083 CCTCAGG
ApaI 1830 GGGCCC GGGCC^C
4690 GGGCCC
ApaLI 1623 GTGCAC G^TGCAC
3874 GTGCAC
BcgI 2466 CGATTTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3866 CGAATTCTGTGC
BsaAI 215 TACGTG [CT]AC^GT[GA]
3686 TACGTG
BsaHI 1604 GACGTC G[GA]^CG[CT]C
2968 GGCGTC
BsgI 2474 GTGCAG GTGCAG(16/14)
2770 GTGCAG
BssSI 2205 CACGAG CACGAG(-5/-1)
2486 CTCGTG
Bsu36I 4836 CCTAAGG CC^T[ACGT]AGG
5083 CCTCAGG
DrdI 2962 GACTCAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3442 GACGATTCAGTC
Eco81I 4836 CCTAAGG CC^T[ACGT]AGG
5083 CCTCAGG
HaeII 2212 GGCGCT [GA]GCGC^[CT]
2891 AGCGCC
HgaI 2969 GCGTC GACGC(5/10)
3326 GCGTC
Hin1I 1604 GACGTC G[GA]^CG[CT]C
2968 GGCGTC
HindIII 2723 AAGCTT A^AGCTT
6421 AAGCTT
NcoI 1974 CCATGG C^CATGG
4181 CCATGG
NdeI 582 CATATG CA^TATG
5203 CATATG
NheI 458 GCTAGC G^CTAGC
1093 GCTAGC
PflMI 976 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3616 CCAAACGATGG
PpuMI 135 AGGACCC [GA]G^G[AT]CC[CT]
946 AGGTCCC
PshAI 1600 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1798 GACCAGAGTC
SacI 900 GAGCTC GAGCT^C
6622 GAGCTC
SapI 1562 GAAGAGC GCTCTTC(1/4)
4069 GCTCTTC
SmlI 1076 CTCAAG C^T[CT][GA]AG
3453 CTTGAG
SpeI 3101 ACTAGT A^CTAGT
6904 ACTAGT
Van91I 976 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3616 CCAAACGATGG
XbaI 5555 TCTAGA T^CTAGA
5692 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BanII BbsI
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoNI EcoO109I
EcoRI EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PshBI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI