################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## Acc65I AclI AflII AgeI Aor51HI AscI Asp718I AspI AvaI BalI BamHI BanI BbeI BbrPI BclI BcnI BfrI BglI BsaBI BsiEI BsiWI BsoBI Bsp1407I BsrGI BssHII Bst1107I BstBI BstZ17I CpoI EagI EclXI Eco47III Eco52I FbaI FseI HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MunI NaeI NarI NciI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SexAI SfiI SfoI SfuI SmaI SnaBI SspBI SspI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 1604 GACGTC GACGT^C AccII 4105 CGCG CG^CG AccIII 6059 TCCGGA T^CCGGA AflIII 3895 ACATGT A^C[GA][CT]GT AhdI 2981 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 2981 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3270 TGCGCA TGC^GCA AvrII 3155 CCTAGG C^CTAGG BbvCI 5043 GCTGAGG CCTCAGC(-5/-2) BciVI 4841 GGATAC GTATCC(6/5) BglII 2438 AGATCT A^GATCT BlnI 3155 CCTAGG C^CTAGG BsaWI 6059 TCCGGA [AT]^CCGG[AT] BseAI 6059 TCCGGA T^CCGGA BspDI 4648 ATCGAT AT^CGAT BspEI 6059 TCCGGA T^CCGGA BspLU11I 3895 ACATGT A^CATGT BsrFI 1207 GCCGGT [GA]^CCGG[CT] BstEII 3034 GGTGACC G^GT[ACGT]ACC BstPI 3034 GGTGACC G^GT[ACGT]ACC BstUI 4105 CGCG CG^CG BstXI 5867 CCACTATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 1207 GCCGGT [GA]^CCGG[CT] ClaI 4648 ATCGAT AT^CGAT DraIII 3935 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 5229 CGGCCA [CT]^GGCC[GA] Eam1105I 2981 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3034 GGTGACC G^GT[ACGT]ACC EcoRV 5585 GATATC GAT^ATC FspI 3270 TGCGCA TGC^GCA MroI 6059 TCCGGA T^CCGGA MvnI 4105 CGCG CG^CG SbfI 482 CCTGCAGG CCTGCA^GG ScaI 4135 AGTACT AGT^ACT SgrAI 1206 CGCCGGTG C[GA]^CCGG[CT]G Sse8387I 482 CCTGCAGG CCTGCA^GG StuI 1817 AGGCCT AGG^CCT XcmI 1185 CCAGACAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 1604 GACGTC G[GA]^CG[CT]C 2968 GGCGTC Alw44I 1623 GTGCAC G^TGCAC 3874 GTGCAC AocI 4836 CCTAAGG CC^T[ACGT]AGG 5083 CCTCAGG ApaI 1830 GGGCCC GGGCC^C 4690 GGGCCC ApaLI 1623 GTGCAC G^TGCAC 3874 GTGCAC BcgI 2466 CGATTTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3866 CGAATTCTGTGC BsaAI 215 TACGTG [CT]AC^GT[GA] 3686 TACGTG BsaHI 1604 GACGTC G[GA]^CG[CT]C 2968 GGCGTC BsgI 2474 GTGCAG GTGCAG(16/14) 2770 GTGCAG BssSI 2205 CACGAG CACGAG(-5/-1) 2486 CTCGTG Bsu36I 4836 CCTAAGG CC^T[ACGT]AGG 5083 CCTCAGG DrdI 2962 GACTCAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3442 GACGATTCAGTC Eco81I 4836 CCTAAGG CC^T[ACGT]AGG 5083 CCTCAGG HaeII 2212 GGCGCT [GA]GCGC^[CT] 2891 AGCGCC HgaI 2969 GCGTC GACGC(5/10) 3326 GCGTC Hin1I 1604 GACGTC G[GA]^CG[CT]C 2968 GGCGTC HindIII 2723 AAGCTT A^AGCTT 6421 AAGCTT NcoI 1974 CCATGG C^CATGG 4181 CCATGG NdeI 582 CATATG CA^TATG 5203 CATATG NheI 458 GCTAGC G^CTAGC 1093 GCTAGC PflMI 976 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3616 CCAAACGATGG PpuMI 135 AGGACCC [GA]G^G[AT]CC[CT] 946 AGGTCCC PshAI 1600 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1798 GACCAGAGTC SacI 900 GAGCTC GAGCT^C 6622 GAGCTC SapI 1562 GAAGAGC GCTCTTC(1/4) 4069 GCTCTTC SmlI 1076 CTCAAG C^T[CT][GA]AG 3453 CTTGAG SpeI 3101 ACTAGT A^CTAGT 6904 ACTAGT Van91I 976 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3616 CCAAACGATGG XbaI 5555 TCTAGA T^CTAGA 5692 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaII BanII BbsI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PshBI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI