##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII Alw44I ApaI ApaLI AscI
AseI AsnI AviII AvrII BbeI
BglI BlnI BsiEI BsiWI BsmBI
BspDI BssHII ClaI CpoI EagI
EclXI Eco52I FseI FspI HpaI
KasI MfeI MluI MunI NaeI
NarI NcoI NgoMIV NotI NruI
PacI PshAI PshBI PvuI RsrII
SalI SapI SbfI SfiI SfoI
SmaI SnaBI Sse8387I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 2256 GGTACC G^GTACC
AocI 1062 CCTGAGG CC^T[ACGT]AGG
Aor51HI 5049 AGCGCT AGC^GCT
Asp718I 2256 GGTACC G^GTACC
AvaI 2074 CTCGAG C^[CT]CG[GA]G
BalI 2432 TGGCCA TGG^CCA
BcgI 652 GCACCATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 3973 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 2074 CTCGAG C^[CT]CG[GA]G
BspLU11I 1798 ACATGT A^CATGT
BsrBI 3197 CCGCTC CCGCTC(-3/-3)
Bst1107I 358 GTATAC GTA^TAC
BstZ17I 358 GTATAC GTA^TAC
Bsu36I 1062 CCTGAGG CC^T[ACGT]AGG
Eco47III 5049 AGCGCT AGC^GCT
Eco81I 1062 CCTGAGG CC^T[ACGT]AGG
EcoRV 1533 GATATC GAT^ATC
FauI 649 CCCGC CCCGC(4/6)
KpnI 2256 GGTACC GGTAC^C
KspI 4333 CCGCGG CCGC^GG
MamI 3973 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2432 TGGCCA TGG^CCA
MscI 2432 TGGCCA TGG^CCA
NdeI 8316 CATATG CA^TATG
PaeR7I 2074 CTCGAG C^TCGAG
PmeI 2245 GTTTAAAC GTTT^AAAC
SacII 4333 CCGCGG CCGC^GG
ScaI 7780 AGTACT AGT^ACT
SexAI 7372 ACCAGGT A^CC[AT]GGT
SphI 3574 GCATGC GCATG^C
SwaI 1576 ATTTAAAT ATTT^AAAT
XhoI 2074 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
AccI 358 GTATAC GT^[AC][GT]AC
3192 GTAGAC
AccIII 1099 TCCGGA T^CCGGA
5188 TCCGGA
AcyI 1119 GACGCC G[GA]^CG[CT]C
7041 GGCGTC
AflII 7065 CTTAAG C^TTAAG
7643 CTTAAG
AflIII 831 ACACGT A^C[GA][CT]GT
1798 ACATGT
AgeI 3677 ACCGGT A^CCGGT
5222 ACCGGT
AhdI 3753 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7629 GACGCTAGGTC
AspEI 3753 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7629 GACGCTAGGTC
AspI 2626 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
7413 GACTTTGTC
BamHI 1613 GGATCC G^GATCC
2147 GGATCC
BanI 2256 GGTACC G^G[CT][GA]CC
3593 GGCACC
BbrPI 832 CACGTG CAC^GTG
1038 CACGTG
BbvCI 2737 CCTCAGC CCTCAGC(-5/-2)
7463 GCTGAGG
BciVI 570 GGATAC GTATCC(6/5)
4701 GTATCC
BfrI 7065 CTTAAG C^TTAAG
7643 CTTAAG
BglII 3807 AGATCT A^GATCT
3972 AGATCT
BsaHI 1119 GACGCC G[GA]^CG[CT]C
7041 GGCGTC
BseAI 1099 TCCGGA T^CCGGA
5188 TCCGGA
BsmI 1446 GAATGC GAATGC(1/-1)
2822 GCATTC
BspEI 1099 TCCGGA T^CCGGA
5188 TCCGGA
BsrFI 3677 ACCGGT [GA]^CCGG[CT]
5222 ACCGGT
BssSI 1006 CACGAG CACGAG(-5/-1)
790 CTCGTG
BstBI 3407 TTCGAA TT^CGAA
4263 TTCGAA
BstEII 2931 GGTGACC G^GT[ACGT]ACC
7403 GGTGACC
BstPI 2931 GGTGACC G^GT[ACGT]ACC
7403 GGTGACC
Cfr10I 3677 ACCGGT [GA]^CCGG[CT]
5222 ACCGGT
DrdI 4317 GACTGCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5873 GACTTCTGTGTC
EaeI 2432 TGGCCA [CT]^GGCC[GA]
6278 TGGCCG
Eam1105I 3753 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7629 GACGCTAGGTC
EcoNI 2070 CCTACTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6903 CCTGATCTAGG
EcoO65I 2931 GGTGACC G^GT[ACGT]ACC
7403 GGTGACC
Hin1I 1119 GACGCC G[GA]^CG[CT]C
7041 GGCGTC
MroI 1099 TCCGGA T^CCGGA
5188 TCCGGA
NheI 6008 GCTAGC G^CTAGC
7749 GCTAGC
NspV 3407 TTCGAA TT^CGAA
4263 TTCGAA
PflFI 2626 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
7413 GACTTTGTC
PinAI 3677 ACCGGT A^CCGGT
5222 ACCGGT
PmaCI 832 CACGTG CAC^GTG
1038 CACGTG
PmlI 832 CACGTG CAC^GTG
1038 CACGTG
SfuI 3407 TTCGAA TT^CGAA
4263 TTCGAA
SgrAI 3676 CACCGGTG C[GA]^CCGG[CT]G
5221 CACCGGTG
SpeI 2307 ACTAGT A^CTAGT
2349 ACTAGT
StuI 4633 AGGCCT AGG^CCT
7214 AGGCCT
Tth111I 2626 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
7413 GACTTTGTC
XcmI 608 CCAGTGGTTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2288 CCACTTACTAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AclI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BanII BbsI BbvI
BclI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspHI BspMI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EarI EcoO109I
EcoRI EcoRII EcoT14I EcoT22I FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PpuMI Psp1406I
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XhoII
XmnI