##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AccIII AclI AgeI Aor51HI ApaI
AscI AvaI AviII BalI BbeI
BcnI BglI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrFI BssHII BssSI BstAPI
BstBI BstXI CfoI Cfr10I ClaI
CpoI DrdI EaeI EagI EclXI
Eco47III Eco52I FseI FspI HaeII
HapII HgaI HhaI HinP1I HpaI
HpaII KasI KspI MamI MfeI
MluNI MroI MscI MspA1I MspI
MunI NaeI NarI NciI NgoMIV
NotI NruI NspV PaeR7I PinAI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SfiI SfoI
SfuI SgrAI SmaI SphI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 3147 GACGTC GACGT^C
Acc65I 3535 GGTACC G^GTACC
AccII 1121 CGCG CG^CG
AcyI 3147 GACGTC G[GA]^CG[CT]C
AhdI 5475 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1524 GTGCAC G^TGCAC
ApaLI 1524 GTGCAC G^TGCAC
Asp718I 3535 GGTACC G^GTACC
AspEI 5475 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4181 CCTAGG C^CTAGG
BamHI 5406 GGATCC G^GATCC
BbrPI 1409 CACGTG CAC^GTG
BclI 6191 TGATCA T^GATCA
BlnI 4181 CCTAGG C^CTAGG
BpmI 3789 CTCCAG CTGGAG(16/14)
BsaHI 3147 GACGTC G[GA]^CG[CT]C
BsmI 266 GCATTC GAATGC(1/-1)
BsrBI 536 GAGCGG CCGCTC(-3/-3)
BstEII 1659 GGTAACC G^GT[ACGT]ACC
BstPI 1659 GGTAACC G^GT[ACGT]ACC
BstUI 1121 CGCG CG^CG
DraIII 1876 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 5475 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1790 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1659 GGTAACC G^GT[ACGT]ACC
EcoRI 4880 GAATTC G^AATTC
EcoRV 4292 GATATC GAT^ATC
FbaI 6191 TGATCA T^GATCA
Hin1I 3147 GACGTC G[GA]^CG[CT]C
KpnI 3535 GGTACC GGTAC^C
MluI 1120 ACGCGT A^CGCGT
MvnI 1121 CGCG CG^CG
NdeI 2954 CATATG CA^TATG
NheI 6033 GCTAGC G^CTAGC
PacI 4750 TTAATTAA TTAAT^TAA
PflMI 3511 CCATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1409 CACGTG CAC^GTG
PmeI 6108 GTTTAAAC GTTT^AAAC
PmlI 1409 CACGTG CAC^GTG
SbfI 5411 CCTGCAGG CCTGCA^GG
ScaI 609 AGTACT AGT^ACT
SnaBI 4095 TACGTA TAC^GTA
SpeI 4469 ACTAGT A^CTAGT
Sse8387I 5411 CCTGCAGG CCTGCA^GG
StuI 3094 AGGCCT AGG^CCT
SwaI 5740 ATTTAAAT ATTT^AAAT
Van91I 3511 CCATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 3045 CCTTAGG CC^T[ACGT]AGG
3097 CCTGAGG
Asp700I 789 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5654 GAAGTTTTTC
AspI 1787 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1859 GACAAAGTC
BanI 1818 GGCACC G^G[CT][GA]CC
3535 GGTACC
BanII 350 GGGCTC G[GA]GC[CT]^C
504 GGGCTC
BbvCI 2632 CCTCAGC CCTCAGC(-5/-2)
5180 CCTCAGC
BcgI 4200 GCAACTCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5242 GCAGTGCTCTCG
BciVI 3865 GTATCC GTATCC(6/5)
6831 GTATCC
BsaI 476 GGTCTC GGTCTC(1/5)
5262 GGTCTC
BspHI 809 TCATGA T^CATGA
6348 TCATGA
Bsu36I 3045 CCTTAGG CC^T[ACGT]AGG
3097 CCTGAGG
Eco81I 3045 CCTTAGG CC^T[ACGT]AGG
3097 CCTGAGG
FauI 5443 CCCGC CCCGC(4/6)
5370 GCGGG
PflFI 1787 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1859 GACAAAGTC
PpuMI 289 AGGACCC [GA]G^G[AT]CC[CT]
1448 AGGACCT
RcaI 809 TCATGA T^CATGA
6348 TCATGA
SexAI 4537 ACCAGGT A^CC[AT]GGT
6198 ACCAGGT
Tth111I 1787 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1859 GACAAAGTC
XcmI 355 CCAAACCTCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2702 CCATGGTCTAGATGG
XmnI 789 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5654 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AseI AsnI AspHI AvaII BbsI
BbvI BfaI BfrI BglII BlpI
BmyI Bpu1102I BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I
BspMI BsrDI BsrGI BsrI BssKI
Bst1107I BstF5I BstNI BstYI BstZ17I
Cac8I CelII Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HincII HindII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI PshBI PstI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII