################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AccIII AclI AgeI Aor51HI ApaI AscI AvaI AviII BalI BbeI BcnI BglI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrFI BssHII BssSI BstAPI BstBI BstXI CfoI Cfr10I ClaI CpoI DrdI EaeI EagI EclXI Eco47III Eco52I FseI FspI HaeII HapII HgaI HhaI HinP1I HpaI HpaII KasI KspI MamI MfeI MluNI MroI MscI MspA1I MspI MunI NaeI NarI NciI NgoMIV NotI NruI NspV PaeR7I PinAI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SfiI SfoI SfuI SgrAI SmaI SphI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 3147 GACGTC GACGT^C Acc65I 3535 GGTACC G^GTACC AccII 1121 CGCG CG^CG AcyI 3147 GACGTC G[GA]^CG[CT]C AhdI 5475 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1524 GTGCAC G^TGCAC ApaLI 1524 GTGCAC G^TGCAC Asp718I 3535 GGTACC G^GTACC AspEI 5475 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 4181 CCTAGG C^CTAGG BamHI 5406 GGATCC G^GATCC BbrPI 1409 CACGTG CAC^GTG BclI 6191 TGATCA T^GATCA BlnI 4181 CCTAGG C^CTAGG BpmI 3789 CTCCAG CTGGAG(16/14) BsaHI 3147 GACGTC G[GA]^CG[CT]C BsmI 266 GCATTC GAATGC(1/-1) BsrBI 536 GAGCGG CCGCTC(-3/-3) BstEII 1659 GGTAACC G^GT[ACGT]ACC BstPI 1659 GGTAACC G^GT[ACGT]ACC BstUI 1121 CGCG CG^CG DraIII 1876 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 5475 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1790 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1659 GGTAACC G^GT[ACGT]ACC EcoRI 4880 GAATTC G^AATTC EcoRV 4292 GATATC GAT^ATC FbaI 6191 TGATCA T^GATCA Hin1I 3147 GACGTC G[GA]^CG[CT]C KpnI 3535 GGTACC GGTAC^C MluI 1120 ACGCGT A^CGCGT MvnI 1121 CGCG CG^CG NdeI 2954 CATATG CA^TATG NheI 6033 GCTAGC G^CTAGC PacI 4750 TTAATTAA TTAAT^TAA PflMI 3511 CCATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1409 CACGTG CAC^GTG PmeI 6108 GTTTAAAC GTTT^AAAC PmlI 1409 CACGTG CAC^GTG SbfI 5411 CCTGCAGG CCTGCA^GG ScaI 609 AGTACT AGT^ACT SnaBI 4095 TACGTA TAC^GTA SpeI 4469 ACTAGT A^CTAGT Sse8387I 5411 CCTGCAGG CCTGCA^GG StuI 3094 AGGCCT AGG^CCT SwaI 5740 ATTTAAAT ATTT^AAAT Van91I 3511 CCATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AocI 3045 CCTTAGG CC^T[ACGT]AGG 3097 CCTGAGG Asp700I 789 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5654 GAAGTTTTTC AspI 1787 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1859 GACAAAGTC BanI 1818 GGCACC G^G[CT][GA]CC 3535 GGTACC BanII 350 GGGCTC G[GA]GC[CT]^C 504 GGGCTC BbvCI 2632 CCTCAGC CCTCAGC(-5/-2) 5180 CCTCAGC BcgI 4200 GCAACTCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5242 GCAGTGCTCTCG BciVI 3865 GTATCC GTATCC(6/5) 6831 GTATCC BsaI 476 GGTCTC GGTCTC(1/5) 5262 GGTCTC BspHI 809 TCATGA T^CATGA 6348 TCATGA Bsu36I 3045 CCTTAGG CC^T[ACGT]AGG 3097 CCTGAGG Eco81I 3045 CCTTAGG CC^T[ACGT]AGG 3097 CCTGAGG FauI 5443 CCCGC CCCGC(4/6) 5370 GCGGG PflFI 1787 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1859 GACAAAGTC PpuMI 289 AGGACCC [GA]G^G[AT]CC[CT] 1448 AGGACCT RcaI 809 TCATGA T^CATGA 6348 TCATGA SexAI 4537 ACCAGGT A^CC[AT]GGT 6198 ACCAGGT Tth111I 1787 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1859 GACAAAGTC XcmI 355 CCAAACCTCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2702 CCATGGTCTAGATGG XmnI 789 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5654 GAAGTTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaII BbsI BbvI BfaI BfrI BglII BlpI BmyI Bpu1102I BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I BspMI BsrDI BsrGI BsrI BssKI Bst1107I BstF5I BstNI BstYI BstZ17I Cac8I CelII Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PshBI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII