##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvrII BalI BamHI BbeI
BcgI BclI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI Hin1I
HpaI KasI KpnI KspI MamI
MluI MluNI MroI MscI MspA1I
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 2235 CGCG CG^CG
AclI 2064 AACGTT AA^CGTT
AflII 2640 CTTAAG C^TTAAG
AlwNI 677 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 2349 ATTAAT AT^TAAT
AsnI 2349 ATTAAT AT^TAAT
Asp700I 1143 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 347 TGCGCA TGC^GCA
BanI 2550 GGTGCC G^G[CT][GA]CC
BbrPI 1518 CACGTG CAC^GTG
BciVI 2078 GGATAC GTATCC(6/5)
BfrI 2640 CTTAAG C^TTAAG
BsaAI 1518 CACGTG [CT]AC^GT[GA]
BsaI 388 GAGACC GGTCTC(1/5)
BspHI 1139 TCATGA T^CATGA
BstUI 2235 CGCG CG^CG
BstXI 2567 CCAAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoT14I 1932 CCATGG C^C[AT][AT]GG
EcoT22I 566 ATGCAT ATGCA^T
FauI 781 GCGGG CCCGC(4/6)
FspI 347 TGCGCA TGC^GCA
HapII 319 CCGG C^CGG
HgaI 1222 GACGC GACGC(5/10)
HincII 531 GTTGAC GT[CT]^[GA]AC
HindII 531 GTTGAC GT[CT]^[GA]AC
HindIII 520 AAGCTT A^AGCTT
HpaII 319 CCGG C^CGG
MfeI 2343 CAATTG C^AATTG
MslI 291 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 319 CCGG C^CGG
MunI 2343 CAATTG C^AATTG
MvnI 2235 CGCG CG^CG
NcoI 1932 CCATGG C^CATGG
NsiI 566 ATGCAT ATGCA^T
PmaCI 1518 CACGTG CAC^GTG
PmlI 1518 CACGTG CAC^GTG
PshAI 534 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2349 ATTAAT AT^TAAT
Psp1406I 2064 AACGTT AA^CGTT
RcaI 1139 TCATGA T^CATGA
SacI 841 GAGCTC GAGCT^C
SmaI 318 CCCGGG CCC^GGG
StyI 1932 CCATGG C^C[AT][AT]GG
XcmI 816 CCAGCAGACCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 318 CCCGGG C^CCGGG
XmaI 318 CCCGGG C^CCGGG
XmnI 1143 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 1107 GATCC GGATC(4/5)
2185 GGATC
AvaI 26 CTCGGG C^[CT]CG[GA]G
318 CCCGGG
BbvCI 1180 GCTGAGG CCTCAGC(-5/-2)
1294 GCTGAGG
BcnI 318 CCCGG CC^[GC]GG
319 CCGGG
BpmI 245 CTGGAG CTGGAG(16/14)
445 CTGGAG
BsmFI 878 GGGAC GGGAC(10/14)
870 GTCCC
BsmI 308 GAATGC GAATGC(1/-1)
400 GCATTC
BsoBI 26 CTCGGG C^[CT]CG[GA]G
318 CCCGGG
BspLU11I 1227 ACATGT A^CATGT
1962 ACATGT
BstYI 1106 AGATCC [GA]^GATC[CT]
2185 GGATCT
DraII 878 GGGACCC [GA]G^G[ACGT]CC[CT]
1184 AGGTCCT
EcoO109I 878 GGGACCC [GA]G^G[ACGT]CC[CT]
1184 AGGTCCT
HaeII 6 AGCGCC [GA]GCGC^[CT]
431 GGCGCT
MflI 1106 AGATCC [GA]^GATC[CT]
2185 GGATCT
NciI 318 CCCGG CC^[GC]GG
319 CCGGG
PpuMI 878 GGGACCC [GA]G^G[AT]CC[CT]
1184 AGGTCCT
PstI 856 CTGCAG CTGCA^G
1535 CTGCAG
SapI 1195 GAAGAGC GCTCTTC(1/4)
483 GCTCTTC
StuI 1145 AGGCCT AGG^CCT
1624 AGGCCT
Tsp45I 1328 GTGAC ^GT[GC]AC
2135 GTCAC
XhoII 1106 AGATCC [GA]^GATC[CT]
2185 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApoI AspHI AvaII BanII BbsI
BbvI BfaI BmyI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrDI BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EarI
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TfiI Tru9I TseI Tsp509I
TspRI