################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccI AccIII AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvrII BalI BamHI BbeI BcgI BclI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI Hin1I HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 2235 CGCG CG^CG AclI 2064 AACGTT AA^CGTT AflII 2640 CTTAAG C^TTAAG AlwNI 677 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 2349 ATTAAT AT^TAAT AsnI 2349 ATTAAT AT^TAAT Asp700I 1143 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 347 TGCGCA TGC^GCA BanI 2550 GGTGCC G^G[CT][GA]CC BbrPI 1518 CACGTG CAC^GTG BciVI 2078 GGATAC GTATCC(6/5) BfrI 2640 CTTAAG C^TTAAG BsaAI 1518 CACGTG [CT]AC^GT[GA] BsaI 388 GAGACC GGTCTC(1/5) BspHI 1139 TCATGA T^CATGA BstUI 2235 CGCG CG^CG BstXI 2567 CCAAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EcoT14I 1932 CCATGG C^C[AT][AT]GG EcoT22I 566 ATGCAT ATGCA^T FauI 781 GCGGG CCCGC(4/6) FspI 347 TGCGCA TGC^GCA HapII 319 CCGG C^CGG HgaI 1222 GACGC GACGC(5/10) HincII 531 GTTGAC GT[CT]^[GA]AC HindII 531 GTTGAC GT[CT]^[GA]AC HindIII 520 AAGCTT A^AGCTT HpaII 319 CCGG C^CGG MfeI 2343 CAATTG C^AATTG MslI 291 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 319 CCGG C^CGG MunI 2343 CAATTG C^AATTG MvnI 2235 CGCG CG^CG NcoI 1932 CCATGG C^CATGG NsiI 566 ATGCAT ATGCA^T PmaCI 1518 CACGTG CAC^GTG PmlI 1518 CACGTG CAC^GTG PshAI 534 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2349 ATTAAT AT^TAAT Psp1406I 2064 AACGTT AA^CGTT RcaI 1139 TCATGA T^CATGA SacI 841 GAGCTC GAGCT^C SmaI 318 CCCGGG CCC^GGG StyI 1932 CCATGG C^C[AT][AT]GG XcmI 816 CCAGCAGACCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 318 CCCGGG C^CCGGG XmaI 318 CCCGGG C^CCGGG XmnI 1143 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 1107 GATCC GGATC(4/5) 2185 GGATC AvaI 26 CTCGGG C^[CT]CG[GA]G 318 CCCGGG BbvCI 1180 GCTGAGG CCTCAGC(-5/-2) 1294 GCTGAGG BcnI 318 CCCGG CC^[GC]GG 319 CCGGG BpmI 245 CTGGAG CTGGAG(16/14) 445 CTGGAG BsmFI 878 GGGAC GGGAC(10/14) 870 GTCCC BsmI 308 GAATGC GAATGC(1/-1) 400 GCATTC BsoBI 26 CTCGGG C^[CT]CG[GA]G 318 CCCGGG BspLU11I 1227 ACATGT A^CATGT 1962 ACATGT BstYI 1106 AGATCC [GA]^GATC[CT] 2185 GGATCT DraII 878 GGGACCC [GA]G^G[ACGT]CC[CT] 1184 AGGTCCT EcoO109I 878 GGGACCC [GA]G^G[ACGT]CC[CT] 1184 AGGTCCT HaeII 6 AGCGCC [GA]GCGC^[CT] 431 GGCGCT MflI 1106 AGATCC [GA]^GATC[CT] 2185 GGATCT NciI 318 CCCGG CC^[GC]GG 319 CCGGG PpuMI 878 GGGACCC [GA]G^G[AT]CC[CT] 1184 AGGTCCT PstI 856 CTGCAG CTGCA^G 1535 CTGCAG SapI 1195 GAAGAGC GCTCTTC(1/4) 483 GCTCTTC StuI 1145 AGGCCT AGG^CCT 1624 AGGCCT Tsp45I 1328 GTGAC ^GT[GC]AC 2135 GTCAC XhoII 1106 AGATCC [GA]^GATC[CT] 2185 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI ApoI AspHI AvaII BanII BbsI BbvI BfaI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EarI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TfiI Tru9I TseI Tsp509I TspRI