##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccII AccIII AclI AflIII AgeI
AhdI Aor51HI ApaI AscI AspEI
BbeI BbrPI BseAI BsiEI BsiWI
BsmBI BspEI BspLU11I BsrFI BssHII
BstBI BstUI Cfr10I CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI FseI HaeII HpaI KasI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 6966 GACGTC GACGT^C
Acc65I 2885 GGTACC G^GTACC
Alw44I 6483 GTGCAC G^TGCAC
ApaLI 6483 GTGCAC G^TGCAC
Asp718I 2885 GGTACC G^GTACC
AvrII 939 CCTAGG C^CTAGG
BanI 2885 GGTACC G^G[CT][GA]CC
BcgI 8484 CGAGTCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 7045 GGATAC GTATCC(6/5)
BcnI 7490 CCCGG CC^[GC]GG
BglI 1786 GCCTCTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 7972 AGATCT A^GATCT
BlnI 939 CCTAGG C^CTAGG
BsaAI 3886 CACGTA [CT]AC^GT[GA]
BsaBI 6217 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 910 ATCGAT AT^CGAT
Bst1107I 4871 GTATAC GTA^TAC
BstEII 7163 GGTCACC G^GT[ACGT]ACC
BstPI 7163 GGTCACC G^GT[ACGT]ACC
BstXI 5651 CCAGAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4871 GTATAC GTA^TAC
ClaI 910 ATCGAT AT^CGAT
DraIII 7634 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 7163 GGTCACC G^GT[ACGT]ACC
EcoRV 2092 GATATC GAT^ATC
FauI 1613 GCGGG CCCGC(4/6)
KpnI 2885 GGTACC GGTAC^C
MamI 6217 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 7490 CCCGG CC^[GC]GG
PshAI 7644 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 7964 ACCTGGT A^CC[AT]GGT
SpeI 3214 ACTAGT A^CTAGT
StuI 4809 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 3771 GGCGTC G[GA]^CG[CT]C
6966 GACGTC
AocI 4774 CCTGAGG CC^T[ACGT]AGG
7501 CCTCAGG
AseI 1023 ATTAAT AT^TAAT
1445 ATTAAT
AsnI 1023 ATTAAT AT^TAAT
1445 ATTAAT
AvaI 6042 CCCGAG C^[CT]CG[GA]G
8482 CCCGAG
AviII 3752 TGCGCA TGC^GCA
3852 TGCGCA
BamHI 2420 GGATCC G^GATCC
4262 GGATCC
BbvCI 1781 CCTCAGC CCTCAGC(-5/-2)
6751 CCTCAGC
BclI 5072 TGATCA T^GATCA
8275 TGATCA
BlpI 7009 GCTCAGC GC^T[ACGT]AGC
7095 GCTGAGC
Bpu1102I 7009 GCTCAGC GC^T[ACGT]AGC
7095 GCTGAGC
BsaHI 3771 GGCGTC G[GA]^CG[CT]C
6966 GACGTC
BsaWI 2121 ACCGGA [AT]^CCGG[AT]
8488 TCCGGT
BsoBI 6042 CCCGAG C^[CT]CG[GA]G
8482 CCCGAG
BspMI 1134 ACCTGC ACCTGC(4/8)
8002 ACCTGC
BsrBI 7596 CCGCTC CCGCTC(-3/-3)
1611 GAGCGG
BsrDI 4306 CATTGC GCAATG(2/0)
7689 CATTGC
BssSI 3840 CTCGTG CACGAG(-5/-1)
6020 CTCGTG
BstAPI 203 GCAGACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6508 GCATCTTATGC
Bsu36I 4774 CCTGAGG CC^T[ACGT]AGG
7501 CCTCAGG
CelII 7009 GCTCAGC GC^T[ACGT]AGC
7095 GCTGAGC
Eco81I 4774 CCTGAGG CC^T[ACGT]AGG
7501 CCTCAGG
EcoRI 2279 GAATTC G^AATTC
7231 GAATTC
FbaI 5072 TGATCA T^GATCA
8275 TGATCA
FspI 3752 TGCGCA TGC^GCA
3852 TGCGCA
HgaI 7153 GACGC GACGC(5/10)
3772 GCGTC
Hin1I 3771 GGCGTC G[GA]^CG[CT]C
6966 GACGTC
HincII 5379 GTCAAC GT[CT]^[GA]AC
8507 GTTGAC
HindII 5379 GTCAAC GT[CT]^[GA]AC
8507 GTTGAC
HindIII 3315 AAGCTT A^AGCTT
6289 AAGCTT
PacI 3319 TTAATTAA TTAAT^TAA
8590 TTAATTAA
PpuMI 1979 AGGACCC [GA]G^G[AT]CC[CT]
2896 GGGTCCT
PshBI 1023 ATTAAT AT^TAAT
1445 ATTAAT
SacI 2752 GAGCTC GAGCT^C
7456 GAGCTC
SapI 1120 GCTCTTC GCTCTTC(1/4)
4046 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI AlwNI ApoI Asp700I
AspHI AspI AvaII BalI BanII
BbsI BbvI BfaI BfrI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeI NdeII NlaIII
NlaIV NsiI NspI PflFI PflMI
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SphI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoII XmnI