##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp718I
AviII BcgI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BssHII BssSI
BstBI ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoRV FseI
FspI HpaI KpnI KspI MluI
MroI NaeI NgoMIV NotI NruI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SbfI
SfiI SfuI SgrAI SnaBI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AocI 2998 CCTCAGG CC^T[ACGT]AGG
Asp700I 3539 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 4397 CCTAGG C^CTAGG
BbeI 7429 GGCGCC GGCGC^C
BbrPI 4549 CACGTG CAC^GTG
BciVI 2274 GTATCC GTATCC(6/5)
BclI 2069 TGATCA T^GATCA
BglI 4157 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4397 CCTAGG C^CTAGG
BlpI 4532 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4532 GCTGAGC GC^T[ACGT]AGC
BsiEI 37 CGGTCG CG[GA][CT]^CG
BspMI 1772 ACCTGC ACCTGC(4/8)
Bst1107I 3786 GTATAC GTA^TAC
BstZ17I 3786 GTATAC GTA^TAC
Bsu36I 2998 CCTCAGG CC^T[ACGT]AGG
CelII 4532 GCTGAGC GC^T[ACGT]AGC
Eco81I 2998 CCTCAGG CC^T[ACGT]AGG
EcoNI 2318 CCTTGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1735 GAATTC G^AATTC
FbaI 2069 TGATCA T^GATCA
KasI 7429 GGCGCC G^GCGCC
MfeI 7390 CAATTG C^AATTG
MunI 7390 CAATTG C^AATTG
NarI 7429 GGCGCC GG^CGCC
NdeI 4023 CATATG CA^TATG
PaeR7I 302 CTCGAG C^TCGAG
PmaCI 4549 CACGTG CAC^GTG
PmlI 4549 CACGTG CAC^GTG
PshAI 259 GACGTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 728 GTCGAC G^TCGAC
SexAI 1652 ACCTGGT A^CC[AT]GGT
SfoI 7429 GGCGCC GGC^GCC
SspI 5694 AATATT AAT^ATT
StuI 3062 AGGCCT AGG^CCT
XbaI 4631 TCTAGA T^CTAGA
XhoI 302 CTCGAG C^TCGAG
XmnI 3539 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 1823 CGCG CG^CG
2079 CGCG
AflII 5054 CTTAAG C^TTAAG
6419 CTTAAG
AhdI 592 GACGTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
925 GACAGCTGGTC
AspEI 592 GACGTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
925 GACAGCTGGTC
AspI 731 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4251 GACCATGTC
BfrI 5054 CTTAAG C^TTAAG
6419 CTTAAG
BsaAI 4549 CACGTG [CT]AC^GT[GA]
5233 CACGTA
BsaBI 526 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3187 GATGGTTATC
Bsp1407I 2395 TGTACA T^GTACA
3607 TGTACA
BsrDI 363 CATTGC GCAATG(2/0)
2214 CATTGC
BsrFI 3138 GCCGGT [GA]^CCGG[CT]
6437 ACCGGC
BsrGI 2395 TGTACA T^GTACA
3607 TGTACA
BstEII 2350 GGTCACC G^GT[ACGT]ACC
5704 GGTAACC
BstPI 2350 GGTCACC G^GT[ACGT]ACC
5704 GGTAACC
BstUI 1823 CGCG CG^CG
2079 CGCG
Cfr10I 3138 GCCGGT [GA]^CCGG[CT]
6437 ACCGGC
Eam1105I 592 GACGTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
925 GACAGCTGGTC
EcoO65I 2350 GGTCACC G^GT[ACGT]ACC
5704 GGTAACC
EcoT22I 3988 ATGCAT ATGCA^T
5222 ATGCAT
HaeII 3830 AGCGCC [GA]GCGC^[CT]
7429 GGCGCC
HincII 728 GTCGAC GT[CT]^[GA]AC
4355 GTTGAC
HindII 728 GTCGAC GT[CT]^[GA]AC
4355 GTTGAC
MamI 526 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3187 GATGGTTATC
MvnI 1823 CGCG CG^CG
2079 CGCG
NheI 1885 GCTAGC G^CTAGC
2581 GCTAGC
NsiI 3988 ATGCAT ATGCA^T
5222 ATGCAT
PflFI 731 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4251 GACCATGTC
SapI 1644 GAAGAGC GCTCTTC(1/4)
6126 GAAGAGC
ScaI 3018 AGTACT AGT^ACT
4286 AGTACT
SmaI 2208 CCCGGG CCC^GGG
3442 CCCGGG
SspBI 2395 TGTACA T^GTACA
3607 TGTACA
Tth111I 731 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4251 GACCATGTC
XmaCI 2208 CCCGGG C^CCGGG
3442 CCCGGG
XmaI 2208 CCCGGG C^CCGGG
3442 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspLU11I BsrBI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SpeI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII