##################################
# NO CUT ENZYMES (   47 enzymes) #
##################################

AccIII      AclI        AgeI        Aor51HI     AscI        
Asp700I     BbeI        BbvCI       BcgI        BseAI       
BsiWI       BspDI       BspEI       BspLU11I    BstBI       
ClaI        CpoI        Eco47III    EcoNI       EcoRV       
FseI        HaeII       HincII      HindII      HpaI        
KasI        KspI        MroI        NarI        NruI        
NspV        PacI        PinAI       PmeI        Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
SfiI        SfoI        SfuI        SphI        Sse8387I    
SwaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I       2390  GGTACC               G^GTACC
Alw44I       5444  GTGCAC               G^TGCAC
ApaI         2579  GGGCCC               GGGCC^C
ApaLI        5444  GTGCAC               G^TGCAC
Asp718I      2390  GGTACC               G^GTACC
AspI          439  GACTAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII          30  TGCGCA               TGC^GCA
AvrII         354  CCTAGG               C^CTAGG
BamHI         537  GGATCC               G^GATCC
BbrPI         381  CACGTG               CAC^GTG
BlnI          354  CCTAGG               C^CTAGG
BsaBI        4368  GATATTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         7921  GAGACC               GGTCTC(1/5)
BsaWI          48  TCCGGT               [AT]^CCGG[AT]
BspHI        5936  TCATGA               T^CATGA
BsrBI          76  GAGCGG               CCGCTC(-3/-3)
BsrFI         297  GCCGGC               [GA]^CCGG[CT]
BstAPI       5627  GCATGTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2953  GGTCACC              G^GT[ACGT]ACC
BstPI        2953  GGTCACC              G^GT[ACGT]ACC
Cfr10I        297  GCCGGC               [GA]^CCGG[CT]
DraIII        257  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          497  GACAGACCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          290  CGGCCG               C^GGCCG
EclXI         290  CGGCCG               C^GGCCG
Eco52I        290  CGGCCG               C^GGCCG
EcoO65I      2953  GGTCACC              G^GT[ACGT]ACC
EcoRI        6222  GAATTC               G^AATTC
FspI           30  TGCGCA               TGC^GCA
KpnI         2390  GGTACC               GGTAC^C
MamI         4368  GATATTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         2367  ACGCGT               A^CGCGT
NaeI          297  GCCGGC               GCC^GGC
NdeI         7979  CATATG               CA^TATG
NgoMIV        297  GCCGGC               G^CCGGC
NotI          289  GCGGCCGC             GC^GGCCGC
PaeR7I       3800  CTCGAG               C^TCGAG
PflFI         439  GACTAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         381  CACGTG               CAC^GTG
PmlI          381  CACGTG               CAC^GTG
PpuMI        2090  AGGTCCT              [GA]G^G[AT]CC[CT]
RcaI         5936  TCATGA               T^CATGA
SapI         5739  GAAGAGC              GCTCTTC(1/4)
SexAI         968  ACCAGGT              A^CC[AT]GGT
SgrAI         296  CGCCGGCG             C[GA]^CCGG[CT]G
SnaBI        8063  TACGTA               TAC^GTA
Tth111I       439  GACTAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         3800  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AatII        3341  GACGTC               GACGT^C
             7389  GACGTC              
AflII        4169  CTTAAG               C^TTAAG
             5526  CTTAAG              
AflIII        727  ACGTGT               A^C[GA][CT]GT
             2367  ACGCGT              
AhdI         1677  GACAACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4707  GACAGACCGTC         
AocI         6423  CCTAAGG              CC^T[ACGT]AGG
             6484  CCTCAGG             
AspEI        1677  GACAACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4707  GACAGACCGTC         
BalI         2027  TGGCCA               TGG^CCA
             5515  TGGCCA              
BclI         6371  TGATCA               T^GATCA
             6886  TGATCA              
BfrI         4169  CTTAAG               C^TTAAG
             5526  CTTAAG              
BglI          292  GCCGCGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2615  GCCATTTCGGC         
BglII        4482  AGATCT               A^GATCT
             4876  AGATCT              
BsaAI         381  CACGTG               [CT]AC^GT[GA]
             8063  TACGTA              
BsgI         8136  CTGCAC               GTGCAG(16/14)
             1744  GTGCAG              
BsiEI          50  CGGTCG               CG[GA][CT]^CG
              290  CGGCCG              
BsrDI        1415  CATTGC               GCAATG(2/0)
             2257  CATTGC              
BssHII        155  GCGCGC               G^CGCGC
              157  GCGCGC              
Bst1107I     6148  GTATAC               GTA^TAC
             8230  GTATAC              
BstZ17I      6148  GTATAC               GTA^TAC
             8230  GTATAC              
Bsu36I       6423  CCTAAGG              CC^T[ACGT]AGG
             6484  CCTCAGG             
DsaI          606  CCATGG               C^C[GA][CT]GG
             6179  CCATGG              
Eam1105I     1677  GACAACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4707  GACAGACCGTC         
Eco81I       6423  CCTAAGG              CC^T[ACGT]AGG
             6484  CCTCAGG             
EcoT22I      2999  ATGCAT               ATGCA^T
             5625  ATGCAT              
FbaI         6371  TGATCA               T^GATCA
             6886  TGATCA              
HindIII      2895  AAGCTT               A^AGCTT
             6032  AAGCTT              
MfeI         7405  CAATTG               C^AATTG
             8151  CAATTG              
MluNI        2027  TGGCCA               TGG^CCA
             5515  TGGCCA              
MscI         2027  TGGCCA               TGG^CCA
             5515  TGGCCA              
MunI         7405  CAATTG               C^AATTG
             8151  CAATTG              
NcoI          606  CCATGG               C^CATGG
             6179  CCATGG              
NsiI         2999  ATGCAT               ATGCA^T
             5625  ATGCAT              
PshAI        4711  GACCGTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             5581  GACTTCAGTC          
PstI         5634  CTGCAG               CTGCA^G
             6826  CTGCAG              
ScaI         6358  AGTACT               AGT^ACT
             6705  AGTACT              
SpeI         5256  ACTAGT               A^CTAGT
             6306  ACTAGT              
StuI         3043  AGGCCT               AGG^CCT
             6481  AGGCCT              
XbaI         4124  TCTAGA               T^CTAGA
             6720  TCTAGA              
XcmI         2812  CCAGCAACCCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             6440  CCAAATCTCAAGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AluI        AlwI        AlwNI       ApoI        
AseI        AsnI        AspHI       AvaI        AvaII       
BanI        BanII       BbsI        BbvI        BciVI       
BcnI        BfaI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspMI       BsrGI       BsrI        BssKI       BssSI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NheI        NlaIII      
NlaIV       NspI        PflMI       PleI        PshBI       
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
SspBI       SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       XmaCI       XmaI