##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AccIII AclI AgeI Aor51HI AscI
Asp700I BbeI BbvCI BcgI BseAI
BsiWI BspDI BspEI BspLU11I BstBI
ClaI CpoI Eco47III EcoNI EcoRV
FseI HaeII HincII HindII HpaI
KasI KspI MroI NarI NruI
NspV PacI PinAI PmeI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SphI Sse8387I
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 2390 GGTACC G^GTACC
Alw44I 5444 GTGCAC G^TGCAC
ApaI 2579 GGGCCC GGGCC^C
ApaLI 5444 GTGCAC G^TGCAC
Asp718I 2390 GGTACC G^GTACC
AspI 439 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 30 TGCGCA TGC^GCA
AvrII 354 CCTAGG C^CTAGG
BamHI 537 GGATCC G^GATCC
BbrPI 381 CACGTG CAC^GTG
BlnI 354 CCTAGG C^CTAGG
BsaBI 4368 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 7921 GAGACC GGTCTC(1/5)
BsaWI 48 TCCGGT [AT]^CCGG[AT]
BspHI 5936 TCATGA T^CATGA
BsrBI 76 GAGCGG CCGCTC(-3/-3)
BsrFI 297 GCCGGC [GA]^CCGG[CT]
BstAPI 5627 GCATGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2953 GGTCACC G^GT[ACGT]ACC
BstPI 2953 GGTCACC G^GT[ACGT]ACC
Cfr10I 297 GCCGGC [GA]^CCGG[CT]
DraIII 257 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 497 GACAGACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 290 CGGCCG C^GGCCG
EclXI 290 CGGCCG C^GGCCG
Eco52I 290 CGGCCG C^GGCCG
EcoO65I 2953 GGTCACC G^GT[ACGT]ACC
EcoRI 6222 GAATTC G^AATTC
FspI 30 TGCGCA TGC^GCA
KpnI 2390 GGTACC GGTAC^C
MamI 4368 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2367 ACGCGT A^CGCGT
NaeI 297 GCCGGC GCC^GGC
NdeI 7979 CATATG CA^TATG
NgoMIV 297 GCCGGC G^CCGGC
NotI 289 GCGGCCGC GC^GGCCGC
PaeR7I 3800 CTCGAG C^TCGAG
PflFI 439 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 381 CACGTG CAC^GTG
PmlI 381 CACGTG CAC^GTG
PpuMI 2090 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 5936 TCATGA T^CATGA
SapI 5739 GAAGAGC GCTCTTC(1/4)
SexAI 968 ACCAGGT A^CC[AT]GGT
SgrAI 296 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 8063 TACGTA TAC^GTA
Tth111I 439 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3800 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AatII 3341 GACGTC GACGT^C
7389 GACGTC
AflII 4169 CTTAAG C^TTAAG
5526 CTTAAG
AflIII 727 ACGTGT A^C[GA][CT]GT
2367 ACGCGT
AhdI 1677 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4707 GACAGACCGTC
AocI 6423 CCTAAGG CC^T[ACGT]AGG
6484 CCTCAGG
AspEI 1677 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4707 GACAGACCGTC
BalI 2027 TGGCCA TGG^CCA
5515 TGGCCA
BclI 6371 TGATCA T^GATCA
6886 TGATCA
BfrI 4169 CTTAAG C^TTAAG
5526 CTTAAG
BglI 292 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2615 GCCATTTCGGC
BglII 4482 AGATCT A^GATCT
4876 AGATCT
BsaAI 381 CACGTG [CT]AC^GT[GA]
8063 TACGTA
BsgI 8136 CTGCAC GTGCAG(16/14)
1744 GTGCAG
BsiEI 50 CGGTCG CG[GA][CT]^CG
290 CGGCCG
BsrDI 1415 CATTGC GCAATG(2/0)
2257 CATTGC
BssHII 155 GCGCGC G^CGCGC
157 GCGCGC
Bst1107I 6148 GTATAC GTA^TAC
8230 GTATAC
BstZ17I 6148 GTATAC GTA^TAC
8230 GTATAC
Bsu36I 6423 CCTAAGG CC^T[ACGT]AGG
6484 CCTCAGG
DsaI 606 CCATGG C^C[GA][CT]GG
6179 CCATGG
Eam1105I 1677 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4707 GACAGACCGTC
Eco81I 6423 CCTAAGG CC^T[ACGT]AGG
6484 CCTCAGG
EcoT22I 2999 ATGCAT ATGCA^T
5625 ATGCAT
FbaI 6371 TGATCA T^GATCA
6886 TGATCA
HindIII 2895 AAGCTT A^AGCTT
6032 AAGCTT
MfeI 7405 CAATTG C^AATTG
8151 CAATTG
MluNI 2027 TGGCCA TGG^CCA
5515 TGGCCA
MscI 2027 TGGCCA TGG^CCA
5515 TGGCCA
MunI 7405 CAATTG C^AATTG
8151 CAATTG
NcoI 606 CCATGG C^CATGG
6179 CCATGG
NsiI 2999 ATGCAT ATGCA^T
5625 ATGCAT
PshAI 4711 GACCGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5581 GACTTCAGTC
PstI 5634 CTGCAG CTGCA^G
6826 CTGCAG
ScaI 6358 AGTACT AGT^ACT
6705 AGTACT
SpeI 5256 ACTAGT A^CTAGT
6306 ACTAGT
StuI 3043 AGGCCT AGG^CCT
6481 AGGCCT
XbaI 4124 TCTAGA T^CTAGA
6720 TCTAGA
XcmI 2812 CCAGCAACCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6440 CCAAATCTCAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AseI AsnI AspHI AvaI AvaII
BanI BanII BbsI BbvI BciVI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NheI NlaIII
NlaIV NspI PflMI PleI PshBI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI