################################## # NO CUT ENZYMES ( 47 enzymes) # ################################## AccIII AclI AgeI Aor51HI AscI Asp700I BbeI BbvCI BcgI BseAI BsiWI BspDI BspEI BspLU11I BstBI ClaI CpoI Eco47III EcoNI EcoRV FseI HaeII HincII HindII HpaI KasI KspI MroI NarI NruI NspV PacI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SphI Sse8387I SwaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 2390 GGTACC G^GTACC Alw44I 5444 GTGCAC G^TGCAC ApaI 2579 GGGCCC GGGCC^C ApaLI 5444 GTGCAC G^TGCAC Asp718I 2390 GGTACC G^GTACC AspI 439 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 30 TGCGCA TGC^GCA AvrII 354 CCTAGG C^CTAGG BamHI 537 GGATCC G^GATCC BbrPI 381 CACGTG CAC^GTG BlnI 354 CCTAGG C^CTAGG BsaBI 4368 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 7921 GAGACC GGTCTC(1/5) BsaWI 48 TCCGGT [AT]^CCGG[AT] BspHI 5936 TCATGA T^CATGA BsrBI 76 GAGCGG CCGCTC(-3/-3) BsrFI 297 GCCGGC [GA]^CCGG[CT] BstAPI 5627 GCATGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2953 GGTCACC G^GT[ACGT]ACC BstPI 2953 GGTCACC G^GT[ACGT]ACC Cfr10I 297 GCCGGC [GA]^CCGG[CT] DraIII 257 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 497 GACAGACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 290 CGGCCG C^GGCCG EclXI 290 CGGCCG C^GGCCG Eco52I 290 CGGCCG C^GGCCG EcoO65I 2953 GGTCACC G^GT[ACGT]ACC EcoRI 6222 GAATTC G^AATTC FspI 30 TGCGCA TGC^GCA KpnI 2390 GGTACC GGTAC^C MamI 4368 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 2367 ACGCGT A^CGCGT NaeI 297 GCCGGC GCC^GGC NdeI 7979 CATATG CA^TATG NgoMIV 297 GCCGGC G^CCGGC NotI 289 GCGGCCGC GC^GGCCGC PaeR7I 3800 CTCGAG C^TCGAG PflFI 439 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 381 CACGTG CAC^GTG PmlI 381 CACGTG CAC^GTG PpuMI 2090 AGGTCCT [GA]G^G[AT]CC[CT] RcaI 5936 TCATGA T^CATGA SapI 5739 GAAGAGC GCTCTTC(1/4) SexAI 968 ACCAGGT A^CC[AT]GGT SgrAI 296 CGCCGGCG C[GA]^CCGG[CT]G SnaBI 8063 TACGTA TAC^GTA Tth111I 439 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3800 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AatII 3341 GACGTC GACGT^C 7389 GACGTC AflII 4169 CTTAAG C^TTAAG 5526 CTTAAG AflIII 727 ACGTGT A^C[GA][CT]GT 2367 ACGCGT AhdI 1677 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4707 GACAGACCGTC AocI 6423 CCTAAGG CC^T[ACGT]AGG 6484 CCTCAGG AspEI 1677 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4707 GACAGACCGTC BalI 2027 TGGCCA TGG^CCA 5515 TGGCCA BclI 6371 TGATCA T^GATCA 6886 TGATCA BfrI 4169 CTTAAG C^TTAAG 5526 CTTAAG BglI 292 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2615 GCCATTTCGGC BglII 4482 AGATCT A^GATCT 4876 AGATCT BsaAI 381 CACGTG [CT]AC^GT[GA] 8063 TACGTA BsgI 8136 CTGCAC GTGCAG(16/14) 1744 GTGCAG BsiEI 50 CGGTCG CG[GA][CT]^CG 290 CGGCCG BsrDI 1415 CATTGC GCAATG(2/0) 2257 CATTGC BssHII 155 GCGCGC G^CGCGC 157 GCGCGC Bst1107I 6148 GTATAC GTA^TAC 8230 GTATAC BstZ17I 6148 GTATAC GTA^TAC 8230 GTATAC Bsu36I 6423 CCTAAGG CC^T[ACGT]AGG 6484 CCTCAGG DsaI 606 CCATGG C^C[GA][CT]GG 6179 CCATGG Eam1105I 1677 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4707 GACAGACCGTC Eco81I 6423 CCTAAGG CC^T[ACGT]AGG 6484 CCTCAGG EcoT22I 2999 ATGCAT ATGCA^T 5625 ATGCAT FbaI 6371 TGATCA T^GATCA 6886 TGATCA HindIII 2895 AAGCTT A^AGCTT 6032 AAGCTT MfeI 7405 CAATTG C^AATTG 8151 CAATTG MluNI 2027 TGGCCA TGG^CCA 5515 TGGCCA MscI 2027 TGGCCA TGG^CCA 5515 TGGCCA MunI 7405 CAATTG C^AATTG 8151 CAATTG NcoI 606 CCATGG C^CATGG 6179 CCATGG NsiI 2999 ATGCAT ATGCA^T 5625 ATGCAT PshAI 4711 GACCGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5581 GACTTCAGTC PstI 5634 CTGCAG CTGCA^G 6826 CTGCAG ScaI 6358 AGTACT AGT^ACT 6705 AGTACT SpeI 5256 ACTAGT A^CTAGT 6306 ACTAGT StuI 3043 AGGCCT AGG^CCT 6481 AGGCCT XbaI 4124 TCTAGA T^CTAGA 6720 TCTAGA XcmI 2812 CCAGCAACCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 6440 CCAAATCTCAAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaI AvaII BanI BanII BbsI BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NheI NlaIII NlaIV NspI PflMI PleI PshBI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI