##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI ApaI AscI Asp718I
AvaI AviII BamHI BbeI BbrPI
BcgI BcnI BfrI BsaAI BsaHI
BsiEI BsiWI BsoBI BsrBI BssHII
Bst1107I BstBI BstUI BstZ17I CpoI
EagI EclXI Eco52I EcoRI EcoRV
FseI FspI Hin1I HpaI KasI
KpnI KspI MfeI MluI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SspI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 4131 TCCGGA T^CCGGA
Alw44I 1212 GTGCAC G^TGCAC
Aor51HI 4126 AGCGCT AGC^GCT
ApaLI 1212 GTGCAC G^TGCAC
AseI 3467 ATTAAT AT^TAAT
AsnI 3467 ATTAAT AT^TAAT
BglI 5663 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 4131 TCCGGA [AT]^CCGG[AT]
BseAI 4131 TCCGGA T^CCGGA
BsmBI 6355 GAGACG CGTCTC(1/5)
Bsp1407I 7002 TGTACA T^GTACA
BspDI 3320 ATCGAT AT^CGAT
BspEI 4131 TCCGGA T^CCGGA
BsrFI 3312 ACCGGC [GA]^CCGG[CT]
BsrGI 7002 TGTACA T^GTACA
BssSI 4236 CTCGTG CACGAG(-5/-1)
BstAPI 6264 GCAAATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3707 GGTGACC G^GT[ACGT]ACC
BstPI 3707 GGTGACC G^GT[ACGT]ACC
Cfr10I 3312 ACCGGC [GA]^CCGG[CT]
ClaI 3320 ATCGAT AT^CGAT
Eco47III 4126 AGCGCT AGC^GCT
EcoO65I 3707 GGTGACC G^GT[ACGT]ACC
FauI 5888 CCCGC CCCGC(4/6)
HindIII 483 AAGCTT A^AGCTT
MroI 4131 TCCGGA T^CCGGA
NdeI 5209 CATATG CA^TATG
PshAI 4137 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3467 ATTAAT AT^TAAT
SapI 7093 GAAGAGC GCTCTTC(1/4)
ScaI 6875 AGTACT AGT^ACT
SpeI 195 ACTAGT A^CTAGT
SspBI 7002 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 544 GTCTAC GT^[AC][GT]AC
4884 GTCTAC
AflIII 2520 ACATGT A^C[GA][CT]GT
3732 ACATGT
AocI 799 CCTCAGG CC^T[ACGT]AGG
3092 CCTGAGG
Asp700I 2821 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4842 GAAGACTTTC
AspI 1099 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
6853 GACATTGTC
BciVI 6789 GGATAC GTATCC(6/5)
1759 GTATCC
BglII 2371 AGATCT A^GATCT
4180 AGATCT
BseRI 165 GAGGAG GAGGAG(10/8)
536 GAGGAG
BspLU11I 2520 ACATGT A^CATGT
3732 ACATGT
Bsu36I 799 CCTCAGG CC^T[ACGT]AGG
3092 CCTGAGG
DraIII 1349 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1604 CACGCAGTG
DrdI 2054 GACTAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6826 GACACAAGTGTC
Eco81I 799 CCTCAGG CC^T[ACGT]AGG
3092 CCTGAGG
EcoT22I 1272 ATGCAT ATGCA^T
1276 ATGCAT
HapII 3313 CCGG C^CGG
4132 CCGG
HgaI 5931 GACGC GACGC(5/10)
7027 GCGTC
HpaII 3313 CCGG C^CGG
4132 CCGG
MaeII 5509 ACGT A^CGT
5615 ACGT
MspI 3313 CCGG C^CGG
4132 CCGG
NsiI 1272 ATGCAT ATGCA^T
1276 ATGCAT
PflFI 1099 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
6853 GACATTGTC
PflMI 2614 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3141 CCAGGTCCTGG
SacI 2239 GAGCTC GAGCT^C
5143 GAGCTC
SexAI 5115 ACCAGGT A^CC[AT]GGT
6990 ACCTGGT
Tth111I 1099 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
6853 GACATTGTC
Van91I 2614 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3141 CCAGGTCCTGG
XmnI 2821 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4842 GAAGACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwI AlwNI ApoI AspEI AspHI
AvaII AvrII BalI BanI BanII
BbsI BbvCI BbvI BclI BfaI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI Eam1105I EarI EcoNI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeII HaeIII HhaI HinP1I HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII