################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AgeI ApaI AscI Asp718I AvaI AviII BamHI BbeI BbrPI BcgI BcnI BfrI BsaAI BsaHI BsiEI BsiWI BsoBI BsrBI BssHII Bst1107I BstBI BstUI BstZ17I CpoI EagI EclXI Eco52I EcoRI EcoRV FseI FspI Hin1I HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccIII 4131 TCCGGA T^CCGGA Alw44I 1212 GTGCAC G^TGCAC Aor51HI 4126 AGCGCT AGC^GCT ApaLI 1212 GTGCAC G^TGCAC AseI 3467 ATTAAT AT^TAAT AsnI 3467 ATTAAT AT^TAAT BglI 5663 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 4131 TCCGGA [AT]^CCGG[AT] BseAI 4131 TCCGGA T^CCGGA BsmBI 6355 GAGACG CGTCTC(1/5) Bsp1407I 7002 TGTACA T^GTACA BspDI 3320 ATCGAT AT^CGAT BspEI 4131 TCCGGA T^CCGGA BsrFI 3312 ACCGGC [GA]^CCGG[CT] BsrGI 7002 TGTACA T^GTACA BssSI 4236 CTCGTG CACGAG(-5/-1) BstAPI 6264 GCAAATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3707 GGTGACC G^GT[ACGT]ACC BstPI 3707 GGTGACC G^GT[ACGT]ACC Cfr10I 3312 ACCGGC [GA]^CCGG[CT] ClaI 3320 ATCGAT AT^CGAT Eco47III 4126 AGCGCT AGC^GCT EcoO65I 3707 GGTGACC G^GT[ACGT]ACC FauI 5888 CCCGC CCCGC(4/6) HindIII 483 AAGCTT A^AGCTT MroI 4131 TCCGGA T^CCGGA NdeI 5209 CATATG CA^TATG PshAI 4137 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3467 ATTAAT AT^TAAT SapI 7093 GAAGAGC GCTCTTC(1/4) ScaI 6875 AGTACT AGT^ACT SpeI 195 ACTAGT A^CTAGT SspBI 7002 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 544 GTCTAC GT^[AC][GT]AC 4884 GTCTAC AflIII 2520 ACATGT A^C[GA][CT]GT 3732 ACATGT AocI 799 CCTCAGG CC^T[ACGT]AGG 3092 CCTGAGG Asp700I 2821 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4842 GAAGACTTTC AspI 1099 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC 6853 GACATTGTC BciVI 6789 GGATAC GTATCC(6/5) 1759 GTATCC BglII 2371 AGATCT A^GATCT 4180 AGATCT BseRI 165 GAGGAG GAGGAG(10/8) 536 GAGGAG BspLU11I 2520 ACATGT A^CATGT 3732 ACATGT Bsu36I 799 CCTCAGG CC^T[ACGT]AGG 3092 CCTGAGG DraIII 1349 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG 1604 CACGCAGTG DrdI 2054 GACTAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6826 GACACAAGTGTC Eco81I 799 CCTCAGG CC^T[ACGT]AGG 3092 CCTGAGG EcoT22I 1272 ATGCAT ATGCA^T 1276 ATGCAT HapII 3313 CCGG C^CGG 4132 CCGG HgaI 5931 GACGC GACGC(5/10) 7027 GCGTC HpaII 3313 CCGG C^CGG 4132 CCGG MaeII 5509 ACGT A^CGT 5615 ACGT MspI 3313 CCGG C^CGG 4132 CCGG NsiI 1272 ATGCAT ATGCA^T 1276 ATGCAT PflFI 1099 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC 6853 GACATTGTC PflMI 2614 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3141 CCAGGTCCTGG SacI 2239 GAGCTC GAGCT^C 5143 GAGCTC SexAI 5115 ACCAGGT A^CC[AT]GGT 6990 ACCTGGT Tth111I 1099 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC 6853 GACATTGTC Van91I 2614 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3141 CCAGGTCCTGG XmnI 2821 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4842 GAAGACTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AciI AcsI AfaI AhdI AluI AlwI AlwNI ApoI AspEI AspHI AvaII AvrII BalI BanI BanII BbsI BbvCI BbvI BclI BfaI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeII HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII