##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII AcyI Aor51HI AscI AviII
AvrII BbeI BbvCI BglI BlnI
BsaHI BsiEI BsiWI BssHII Bst1107I
BstZ17I CpoI EagI EclXI Eco47III
Eco52I EcoT22I FseI FspI Hin1I
KasI KspI MluI NaeI NarI
NgoMIV NotI NsiI PacI PmeI
PvuI RsrII SacII SalI SapI
SexAI SfiI SfoI SgrAI SnaBI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 5024 GTCTAC GT^[AC][GT]AC
AccIII 3895 TCCGGA T^CCGGA
AclI 5759 AACGTT AA^CGTT
Alw44I 6920 GTGCAC G^TGCAC
ApaI 3228 GGGCCC GGGCC^C
ApaLI 6920 GTGCAC G^TGCAC
AseI 6793 ATTAAT AT^TAAT
AsnI 6793 ATTAAT AT^TAAT
BamHI 3674 GGATCC G^GATCC
BbrPI 6573 CACGTG CAC^GTG
BciVI 929 GGATAC GTATCC(6/5)
BsaAI 6573 CACGTG [CT]AC^GT[GA]
BsaI 2297 GGTCTC GGTCTC(1/5)
BseAI 3895 TCCGGA T^CCGGA
BsmBI 5295 GAGACG CGTCTC(1/5)
Bsp1407I 2530 TGTACA T^GTACA
BspEI 3895 TCCGGA T^CCGGA
BsrBI 3655 CCGCTC CCGCTC(-3/-3)
BsrGI 2530 TGTACA T^GTACA
BstAPI 3613 GCAGACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3025 TTCGAA TT^CGAA
BstEII 1562 GGTAACC G^GT[ACGT]ACC
BstPI 1562 GGTAACC G^GT[ACGT]ACC
DraI 1932 TTTAAA TTT^AAA
DraIII 2447 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1562 GGTAACC G^GT[ACGT]ACC
EcoRV 5502 GATATC GAT^ATC
HaeII 4769 GGCGCT [GA]GCGC^[CT]
MfeI 5818 CAATTG C^AATTG
MroI 3895 TCCGGA T^CCGGA
MunI 5818 CAATTG C^AATTG
NspV 3025 TTCGAA TT^CGAA
PaeR7I 3729 CTCGAG C^TCGAG
PflMI 2049 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 6573 CACGTG CAC^GTG
PmlI 6573 CACGTG CAC^GTG
PshAI 507 GACGAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6793 ATTAAT AT^TAAT
Psp1406I 5759 AACGTT AA^CGTT
SbfI 3293 CCTGCAGG CCTGCA^GG
ScaI 1280 AGTACT AGT^ACT
SfuI 3025 TTCGAA TT^CGAA
SmaI 3225 CCCGGG CCC^GGG
SpeI 3840 ACTAGT A^CTAGT
Sse8387I 3293 CCTGCAGG CCTGCA^GG
SspBI 2530 TGTACA T^GTACA
StuI 2965 AGGCCT AGG^CCT
Van91I 2049 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3729 CTCGAG C^TCGAG
XmaCI 3225 CCCGGG C^CCGGG
XmaI 3225 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AgeI 795 ACCGGT A^CCGGT
2101 ACCGGT
AhdI 1263 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2733 GACATCCCGTC
Asp700I 1227 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1941 GAAGTGATTC
AspEI 1263 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2733 GACATCCCGTC
BalI 2046 TGGCCA TGG^CCA
6458 TGGCCA
BglII 232 AGATCT A^GATCT
2498 AGATCT
BsaBI 2110 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6179 GATCCAGATC
BspDI 2817 ATCGAT AT^CGAT
4326 ATCGAT
BspHI 1612 TCATGA T^CATGA
3988 TCATGA
BspLU11I 118 ACATGT A^CATGT
5566 ACATGT
BsrDI 5183 CATTGC GCAATG(2/0)
3403 GCAATG
BssSI 4314 CACGAG CACGAG(-5/-1)
5776 CACGAG
ClaI 2817 ATCGAT AT^CGAT
4326 ATCGAT
DrdI 5132 GACGCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6243 GACAGTCTCGTC
Eam1105I 1263 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2733 GACATCCCGTC
HindIII 499 AAGCTT A^AGCTT
6069 AAGCTT
HpaI 1685 GTTAAC GTT^AAC
2027 GTTAAC
MamI 2110 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6179 GATCCAGATC
MluNI 2046 TGGCCA TGG^CCA
6458 TGGCCA
MscI 2046 TGGCCA TGG^CCA
6458 TGGCCA
NcoI 287 CCATGG C^CATGG
2467 CCATGG
NheI 3926 GCTAGC G^CTAGC
6464 GCTAGC
NruI 2354 TCGCGA TCG^CGA
4984 TCGCGA
PinAI 795 ACCGGT A^CCGGT
2101 ACCGGT
PpuMI 3218 GGGACCC [GA]G^G[AT]CC[CT]
5088 AGGTCCT
RcaI 1612 TCATGA T^CATGA
3988 TCATGA
SphI 3525 GCATGC GCATG^C
5746 GCATGC
XcmI 481 CCAAGGCTTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4450 CCAGCCTCATTCTGG
XmnI 1227 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1941 GAAGTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AflII AflIII AluI AlwI AlwNI
AocI ApoI Asp718I AspHI AspI
AvaI AvaII BanI BanII BbsI
BbvI BcgI BclI BcnI BfaI
BfrI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeI NdeII NlaIII
NlaIV NspI PflFI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII