################################## # NO CUT ENZYMES ( 47 enzymes) # ################################## AatII AcyI Aor51HI AscI AviII AvrII BbeI BbvCI BglI BlnI BsaHI BsiEI BsiWI BssHII Bst1107I BstZ17I CpoI EagI EclXI Eco47III Eco52I EcoT22I FseI FspI Hin1I KasI KspI MluI NaeI NarI NgoMIV NotI NsiI PacI PmeI PvuI RsrII SacII SalI SapI SexAI SfiI SfoI SgrAI SnaBI SwaI XbaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 5024 GTCTAC GT^[AC][GT]AC AccIII 3895 TCCGGA T^CCGGA AclI 5759 AACGTT AA^CGTT Alw44I 6920 GTGCAC G^TGCAC ApaI 3228 GGGCCC GGGCC^C ApaLI 6920 GTGCAC G^TGCAC AseI 6793 ATTAAT AT^TAAT AsnI 6793 ATTAAT AT^TAAT BamHI 3674 GGATCC G^GATCC BbrPI 6573 CACGTG CAC^GTG BciVI 929 GGATAC GTATCC(6/5) BsaAI 6573 CACGTG [CT]AC^GT[GA] BsaI 2297 GGTCTC GGTCTC(1/5) BseAI 3895 TCCGGA T^CCGGA BsmBI 5295 GAGACG CGTCTC(1/5) Bsp1407I 2530 TGTACA T^GTACA BspEI 3895 TCCGGA T^CCGGA BsrBI 3655 CCGCTC CCGCTC(-3/-3) BsrGI 2530 TGTACA T^GTACA BstAPI 3613 GCAGACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3025 TTCGAA TT^CGAA BstEII 1562 GGTAACC G^GT[ACGT]ACC BstPI 1562 GGTAACC G^GT[ACGT]ACC DraI 1932 TTTAAA TTT^AAA DraIII 2447 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 1562 GGTAACC G^GT[ACGT]ACC EcoRV 5502 GATATC GAT^ATC HaeII 4769 GGCGCT [GA]GCGC^[CT] MfeI 5818 CAATTG C^AATTG MroI 3895 TCCGGA T^CCGGA MunI 5818 CAATTG C^AATTG NspV 3025 TTCGAA TT^CGAA PaeR7I 3729 CTCGAG C^TCGAG PflMI 2049 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 6573 CACGTG CAC^GTG PmlI 6573 CACGTG CAC^GTG PshAI 507 GACGAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 6793 ATTAAT AT^TAAT Psp1406I 5759 AACGTT AA^CGTT SbfI 3293 CCTGCAGG CCTGCA^GG ScaI 1280 AGTACT AGT^ACT SfuI 3025 TTCGAA TT^CGAA SmaI 3225 CCCGGG CCC^GGG SpeI 3840 ACTAGT A^CTAGT Sse8387I 3293 CCTGCAGG CCTGCA^GG SspBI 2530 TGTACA T^GTACA StuI 2965 AGGCCT AGG^CCT Van91I 2049 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 3729 CTCGAG C^TCGAG XmaCI 3225 CCCGGG C^CCGGG XmaI 3225 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AgeI 795 ACCGGT A^CCGGT 2101 ACCGGT AhdI 1263 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2733 GACATCCCGTC Asp700I 1227 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1941 GAAGTGATTC AspEI 1263 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2733 GACATCCCGTC BalI 2046 TGGCCA TGG^CCA 6458 TGGCCA BglII 232 AGATCT A^GATCT 2498 AGATCT BsaBI 2110 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 6179 GATCCAGATC BspDI 2817 ATCGAT AT^CGAT 4326 ATCGAT BspHI 1612 TCATGA T^CATGA 3988 TCATGA BspLU11I 118 ACATGT A^CATGT 5566 ACATGT BsrDI 5183 CATTGC GCAATG(2/0) 3403 GCAATG BssSI 4314 CACGAG CACGAG(-5/-1) 5776 CACGAG ClaI 2817 ATCGAT AT^CGAT 4326 ATCGAT DrdI 5132 GACGCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6243 GACAGTCTCGTC Eam1105I 1263 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2733 GACATCCCGTC HindIII 499 AAGCTT A^AGCTT 6069 AAGCTT HpaI 1685 GTTAAC GTT^AAC 2027 GTTAAC MamI 2110 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 6179 GATCCAGATC MluNI 2046 TGGCCA TGG^CCA 6458 TGGCCA MscI 2046 TGGCCA TGG^CCA 6458 TGGCCA NcoI 287 CCATGG C^CATGG 2467 CCATGG NheI 3926 GCTAGC G^CTAGC 6464 GCTAGC NruI 2354 TCGCGA TCG^CGA 4984 TCGCGA PinAI 795 ACCGGT A^CCGGT 2101 ACCGGT PpuMI 3218 GGGACCC [GA]G^G[AT]CC[CT] 5088 AGGTCCT RcaI 1612 TCATGA T^CATGA 3988 TCATGA SphI 3525 GCATGC GCATG^C 5746 GCATGC XcmI 481 CCAAGGCTTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4450 CCAGCCTCATTCTGG XmnI 1227 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1941 GAAGTGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### Acc65I AccII AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI AocI ApoI Asp718I AspHI AspI AvaI AvaII BanI BanII BbsI BbvI BcgI BclI BcnI BfaI BfrI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PflFI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII