##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
AatII AflII AhdI AscI AspEI
AvrII BbeI BfrI BlnI BsrBI
BssHII Bst1107I BstZ17I CpoI DraIII
EagI Eam1105I EclXI Eco52I EcoRV
FauI FseI HpaI KasI KspI
MluI NarI NotI NruI PacI
PmeI PshAI PvuI RsrII SacII
SbfI SfiI SfoI SgrAI SnaBI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 4455 TCCGGA T^CCGGA
AclI 1393 AACGTT AA^CGTT
AcyI 778 GGCGTC G[GA]^CG[CT]C
Alw44I 1535 GTGCAC G^TGCAC
Aor51HI 1700 AGCGCT AGC^GCT
ApaI 2151 GGGCCC GGGCC^C
ApaLI 1535 GTGCAC G^TGCAC
AseI 6783 ATTAAT AT^TAAT
AsnI 6783 ATTAAT AT^TAAT
AspI 1972 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 3055 CGACTCGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4006 GTATCC GTATCC(6/5)
BglII 2547 AGATCT A^GATCT
BlpI 3044 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3044 GCTCAGC GC^T[ACGT]AGC
BsaBI 2749 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 778 GGCGTC G[GA]^CG[CT]C
BseAI 4455 TCCGGA T^CCGGA
BsiEI 2517 CGACCG CG[GA][CT]^CG
BsiWI 901 CGTACG C^GTACG
BspEI 4455 TCCGGA T^CCGGA
BstBI 3816 TTCGAA TT^CGAA
BstEII 2104 GGTGACC G^GT[ACGT]ACC
BstPI 2104 GGTGACC G^GT[ACGT]ACC
CelII 3044 GCTCAGC GC^T[ACGT]AGC
DraI 2847 TTTAAA TTT^AAA
DrdI 1625 GACACAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1700 AGCGCT AGC^GCT
EcoO65I 2104 GGTGACC G^GT[ACGT]ACC
Hin1I 778 GGCGTC G[GA]^CG[CT]C
MamI 2749 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5664 CAATTG C^AATTG
MroI 4455 TCCGGA T^CCGGA
MunI 5664 CAATTG C^AATTG
NaeI 797 GCCGGC GCC^GGC
NdeI 5273 CATATG CA^TATG
NgoMIV 797 GCCGGC G^CCGGC
NheI 3301 GCTAGC G^CTAGC
NspV 3816 TTCGAA TT^CGAA
PaeR7I 2744 CTCGAG C^TCGAG
PflFI 1972 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 6783 ATTAAT AT^TAAT
Psp1406I 1393 AACGTT AA^CGTT
ScaI 1865 AGTACT AGT^ACT
SfuI 3816 TTCGAA TT^CGAA
SpeI 868 ACTAGT A^CTAGT
SphI 2043 GCATGC GCATG^C
Tth111I 1972 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2744 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 2918 GTCGAC GT^[AC][GT]AC
4667 GTCGAC
AgeI 1959 ACCGGT A^CCGGT
6179 ACCGGT
AlwNI 843 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2674 CAGAAACTG
AviII 3948 TGCGCA TGC^GCA
5428 TGCGCA
BamHI 3279 GGATCC G^GATCC
6384 GGATCC
BbrPI 1992 CACGTG CAC^GTG
6338 CACGTG
BsaI 1648 GAGACC GGTCTC(1/5)
4500 GAGACC
BsmI 6148 GAATGC GAATGC(1/-1)
4622 GCATTC
Bsp1407I 2086 TGTACA T^GTACA
2442 TGTACA
BspDI 4660 ATCGAT AT^CGAT
4673 ATCGAT
BspHI 4515 TCATGA T^CATGA
5194 TCATGA
BspMI 2216 ACCTGC ACCTGC(4/8)
3522 ACCTGC
BsrGI 2086 TGTACA T^GTACA
2442 TGTACA
BssSI 4497 CACGAG CACGAG(-5/-1)
2584 CTCGTG
BstAPI 842 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1835 GCACCGCCTGC
ClaI 4660 ATCGAT AT^CGAT
4673 ATCGAT
EcoT22I 441 ATGCAT ATGCA^T
4395 ATGCAT
FspI 3948 TGCGCA TGC^GCA
5428 TGCGCA
HaeII 1700 AGCGCT [GA]GCGC^[CT]
6350 GGCGCT
HgaI 76 GACGC GACGC(5/10)
779 GCGTC
HindIII 3271 AAGCTT A^AGCTT
6326 AAGCTT
NsiI 441 ATGCAT ATGCA^T
4395 ATGCAT
PflMI 427 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
491 CCAACACCTGG
PinAI 1959 ACCGGT A^CCGGT
6179 ACCGGT
PmaCI 1992 CACGTG CAC^GTG
6338 CACGTG
PmlI 1992 CACGTG CAC^GTG
6338 CACGTG
RcaI 4515 TCATGA T^CATGA
5194 TCATGA
SalI 2918 GTCGAC G^TCGAC
4667 GTCGAC
SapI 5612 GCTCTTC GCTCTTC(1/4)
5743 GCTCTTC
SmaI 4148 CCCGGG CCC^GGG
5484 CCCGGG
SspBI 2086 TGTACA T^GTACA
2442 TGTACA
StuI 2091 AGGCCT AGG^CCT
2598 AGGCCT
Van91I 427 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
491 CCAACACCTGG
XbaI 2118 TCTAGA T^CTAGA
3655 TCTAGA
XmaCI 4148 CCCGGG C^CCGGG
5484 CCCGGG
XmaI 4148 CCCGGG C^CCGGG
5484 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AflIII AluI AlwI AocI ApoI
Asp700I Asp718I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BclI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspLU11I BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI