##################################
# NO CUT ENZYMES (   39 enzymes) #
##################################

AccI        AclI        AgeI        Alw44I      ApaLI       
AscI        BsiEI       BsiWI       Bst1107I    BstBI       
BstZ17I     CpoI        EagI        EclXI       Eco52I      
EcoRI       EcoRV       FseI        MluI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmeI        Psp1406I    PvuI        RsrII       SalI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII         302  GACGTC               GACGT^C
Aor51HI      5712  AGCGCT               AGC^GCT
AseI         6902  ATTAAT               AT^TAAT
AsnI         6902  ATTAAT               AT^TAAT
AviII         340  TGCGCA               TGC^GCA
BamHI        6749  GGATCC               G^GATCC
BbeI          193  GGCGCC               GGCGC^C
BclI         4175  TGATCA               T^GATCA
BsmBI        7023  CGTCTC               CGTCTC(1/5)
BspHI         855  TCATGA               T^CATGA
BsrBI        8842  GAGCGG               CCGCTC(-3/-3)
BsrFI         135  GCCGGC               [GA]^CCGG[CT]
BssHII       1517  GCGCGC               G^CGCGC
BssSI        9111  CTCGTG               CACGAG(-5/-1)
BstAPI       8070  GCAGCCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       8227  GGTCACC              G^GT[ACGT]ACC
BstPI        8227  GGTCACC              G^GT[ACGT]ACC
Cfr10I        135  GCCGGC               [GA]^CCGG[CT]
Eco47III     5712  AGCGCT               AGC^GCT
EcoO65I      8227  GGTCACC              G^GT[ACGT]ACC
FbaI         4175  TGATCA               T^GATCA
FspI          340  TGCGCA               TGC^GCA
KasI          193  GGCGCC               G^GCGCC
KspI          252  CCGCGG               CCGC^GG
NaeI          135  GCCGGC               GCC^GGC
NarI          193  GGCGCC               GG^CGCC
NcoI         2610  CCATGG               C^CATGG
NdeI         6850  CATATG               CA^TATG
NgoMIV        135  GCCGGC               G^CCGGC
PshBI        6902  ATTAAT               AT^TAAT
RcaI          855  TCATGA               T^CATGA
SacII         252  CCGCGG               CCGC^GG
SapI         5630  GAAGAGC              GCTCTTC(1/4)
SbfI         7327  CCTGCAGG             CCTGCA^GG
SexAI        2696  ACCTGGT              A^CC[AT]GGT
SfoI          193  GGCGCC               GGC^GCC
Sse8387I     7327  CCTGCAGG             CCTGCA^GG
StuI         8435  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

Acc65I        235  GGTACC               G^GTACC
             8867  GGTACC              
AcyI          193  GGCGCC               G[GA]^CG[CT]C
              302  GACGTC              
AflII        5777  CTTAAG               C^TTAAG
             6284  CTTAAG              
Asp718I       235  GGTACC               G^GTACC
             8867  GGTACC              
AspI         1589  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6690  GACTTGGTC           
BbrPI        2717  CACGTG               CAC^GTG
             4441  CACGTG              
BcgI         4771  CGATCATTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              348  GCATTTACCTCG        
BcnI          223  CCCGG                CC^[GC]GG
             8260  CCGGG               
BfrI         5777  CTTAAG               C^TTAAG
             6284  CTTAAG              
BglI         5814  GCCAGTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             7334  GCCAGGAAGGC         
BglII         784  AGATCT               A^GATCT
             3550  AGATCT              
BlpI         8120  GCTGAGC              GC^T[ACGT]AGC
             8643  GCTGAGC             
Bpu1102I     8120  GCTGAGC              GC^T[ACGT]AGC
             8643  GCTGAGC             
BsaBI        1312  GATTTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             6940  GATTACCATC          
BsaHI         193  GGCGCC               G[GA]^CG[CT]C
              302  GACGTC              
Bsp1407I     2020  TGTACA               T^GTACA
             2058  TGTACA              
BspDI         712  ATCGAT               AT^CGAT
             7443  ATCGAT              
BsrGI        2020  TGTACA               T^GTACA
             2058  TGTACA              
CelII        8120  GCTGAGC              GC^T[ACGT]AGC
             8643  GCTGAGC             
ClaI          712  ATCGAT               AT^CGAT
             7443  ATCGAT              
EcoT22I      1699  ATGCAT               ATGCA^T
             8538  ATGCAT              
Hin1I         193  GGCGCC               G[GA]^CG[CT]C
              302  GACGTC              
HpaI         3733  GTTAAC               GTT^AAC
             5202  GTTAAC              
KpnI          235  GGTACC               GGTAC^C
             8867  GGTACC              
MamI         1312  GATTTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             6940  GATTACCATC          
NciI          223  CCCGG                CC^[GC]GG
             8260  CCGGG               
NheI         5447  GCTAGC               G^CTAGC
             8736  GCTAGC              
NsiI         1699  ATGCAT               ATGCA^T
             8538  ATGCAT              
PflFI        1589  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6690  GACTTGGTC           
PmaCI        2717  CACGTG               CAC^GTG
             4441  CACGTG              
PmlI         2717  CACGTG               CAC^GTG
             4441  CACGTG              
SspBI        2020  TGTACA               T^GTACA
             2058  TGTACA              
SwaI         1703  ATTTAAAT             ATTT^AAAT
             6915  ATTTAAAT            
Tth111I      1589  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6690  GACTTGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  147 enzymes) #
#########################################

AccII       AccIII      AciI        AcsI        AfaI        
AflIII      AhdI        AluI        AlwI        AlwNI       
AocI        ApaI        ApoI        Asp700I     AspEI       
AspHI       AvaI        AvaII       AvrII       BalI        
BanI        BanII       BbsI        BbvCI       BbvI        
BciVI       BfaI        BlnI        BmyI        BpmI        
BpuAI       BsaAI       BsaI        BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspEI       BspLU11I    BspMI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EaeI        Eam1105I    
EarI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MfeI        MflI        
MluNI       MnlI        MroI        MscI        MseI        
MslI        MspA1I      MspI        MunI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PshAI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        SphI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XbaI        XcmI        
XhoII       XmnI