################################## # NO CUT ENZYMES ( 39 enzymes) # ################################## AccI AclI AgeI Alw44I ApaLI AscI BsiEI BsiWI Bst1107I BstBI BstZ17I CpoI EagI EclXI Eco52I EcoRI EcoRV FseI MluI NotI NruI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SalI SfiI SfuI SgrAI SmaI SnaBI SpeI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 302 GACGTC GACGT^C Aor51HI 5712 AGCGCT AGC^GCT AseI 6902 ATTAAT AT^TAAT AsnI 6902 ATTAAT AT^TAAT AviII 340 TGCGCA TGC^GCA BamHI 6749 GGATCC G^GATCC BbeI 193 GGCGCC GGCGC^C BclI 4175 TGATCA T^GATCA BsmBI 7023 CGTCTC CGTCTC(1/5) BspHI 855 TCATGA T^CATGA BsrBI 8842 GAGCGG CCGCTC(-3/-3) BsrFI 135 GCCGGC [GA]^CCGG[CT] BssHII 1517 GCGCGC G^CGCGC BssSI 9111 CTCGTG CACGAG(-5/-1) BstAPI 8070 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 8227 GGTCACC G^GT[ACGT]ACC BstPI 8227 GGTCACC G^GT[ACGT]ACC Cfr10I 135 GCCGGC [GA]^CCGG[CT] Eco47III 5712 AGCGCT AGC^GCT EcoO65I 8227 GGTCACC G^GT[ACGT]ACC FbaI 4175 TGATCA T^GATCA FspI 340 TGCGCA TGC^GCA KasI 193 GGCGCC G^GCGCC KspI 252 CCGCGG CCGC^GG NaeI 135 GCCGGC GCC^GGC NarI 193 GGCGCC GG^CGCC NcoI 2610 CCATGG C^CATGG NdeI 6850 CATATG CA^TATG NgoMIV 135 GCCGGC G^CCGGC PshBI 6902 ATTAAT AT^TAAT RcaI 855 TCATGA T^CATGA SacII 252 CCGCGG CCGC^GG SapI 5630 GAAGAGC GCTCTTC(1/4) SbfI 7327 CCTGCAGG CCTGCA^GG SexAI 2696 ACCTGGT A^CC[AT]GGT SfoI 193 GGCGCC GGC^GCC Sse8387I 7327 CCTGCAGG CCTGCA^GG StuI 8435 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 235 GGTACC G^GTACC 8867 GGTACC AcyI 193 GGCGCC G[GA]^CG[CT]C 302 GACGTC AflII 5777 CTTAAG C^TTAAG 6284 CTTAAG Asp718I 235 GGTACC G^GTACC 8867 GGTACC AspI 1589 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 6690 GACTTGGTC BbrPI 2717 CACGTG CAC^GTG 4441 CACGTG BcgI 4771 CGATCATTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 348 GCATTTACCTCG BcnI 223 CCCGG CC^[GC]GG 8260 CCGGG BfrI 5777 CTTAAG C^TTAAG 6284 CTTAAG BglI 5814 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 7334 GCCAGGAAGGC BglII 784 AGATCT A^GATCT 3550 AGATCT BlpI 8120 GCTGAGC GC^T[ACGT]AGC 8643 GCTGAGC Bpu1102I 8120 GCTGAGC GC^T[ACGT]AGC 8643 GCTGAGC BsaBI 1312 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 6940 GATTACCATC BsaHI 193 GGCGCC G[GA]^CG[CT]C 302 GACGTC Bsp1407I 2020 TGTACA T^GTACA 2058 TGTACA BspDI 712 ATCGAT AT^CGAT 7443 ATCGAT BsrGI 2020 TGTACA T^GTACA 2058 TGTACA CelII 8120 GCTGAGC GC^T[ACGT]AGC 8643 GCTGAGC ClaI 712 ATCGAT AT^CGAT 7443 ATCGAT EcoT22I 1699 ATGCAT ATGCA^T 8538 ATGCAT Hin1I 193 GGCGCC G[GA]^CG[CT]C 302 GACGTC HpaI 3733 GTTAAC GTT^AAC 5202 GTTAAC KpnI 235 GGTACC GGTAC^C 8867 GGTACC MamI 1312 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 6940 GATTACCATC NciI 223 CCCGG CC^[GC]GG 8260 CCGGG NheI 5447 GCTAGC G^CTAGC 8736 GCTAGC NsiI 1699 ATGCAT ATGCA^T 8538 ATGCAT PflFI 1589 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 6690 GACTTGGTC PmaCI 2717 CACGTG CAC^GTG 4441 CACGTG PmlI 2717 CACGTG CAC^GTG 4441 CACGTG SspBI 2020 TGTACA T^GTACA 2058 TGTACA SwaI 1703 ATTTAAAT ATTT^AAAT 6915 ATTTAAAT Tth111I 1589 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 6690 GACTTGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 147 enzymes) # ######################################### AccII AccIII AciI AcsI AfaI AflIII AhdI AluI AlwI AlwNI AocI ApaI ApoI Asp700I AspEI AspHI AvaI AvaII AvrII BalI BanI BanII BbsI BbvCI BbvI BciVI BfaI BlnI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspEI BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII XmnI