##################################
# NO CUT ENZYMES ( 39 enzymes) #
##################################
AccI AclI AgeI Alw44I ApaLI
AscI BsiEI BsiWI Bst1107I BstBI
BstZ17I CpoI EagI EclXI Eco52I
EcoRI EcoRV FseI MluI NotI
NruI NspV PacI PaeR7I PinAI
PmeI Psp1406I PvuI RsrII SalI
SfiI SfuI SgrAI SmaI SnaBI
SpeI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 302 GACGTC GACGT^C
Aor51HI 5712 AGCGCT AGC^GCT
AseI 6902 ATTAAT AT^TAAT
AsnI 6902 ATTAAT AT^TAAT
AviII 340 TGCGCA TGC^GCA
BamHI 6749 GGATCC G^GATCC
BbeI 193 GGCGCC GGCGC^C
BclI 4175 TGATCA T^GATCA
BsmBI 7023 CGTCTC CGTCTC(1/5)
BspHI 855 TCATGA T^CATGA
BsrBI 8842 GAGCGG CCGCTC(-3/-3)
BsrFI 135 GCCGGC [GA]^CCGG[CT]
BssHII 1517 GCGCGC G^CGCGC
BssSI 9111 CTCGTG CACGAG(-5/-1)
BstAPI 8070 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 8227 GGTCACC G^GT[ACGT]ACC
BstPI 8227 GGTCACC G^GT[ACGT]ACC
Cfr10I 135 GCCGGC [GA]^CCGG[CT]
Eco47III 5712 AGCGCT AGC^GCT
EcoO65I 8227 GGTCACC G^GT[ACGT]ACC
FbaI 4175 TGATCA T^GATCA
FspI 340 TGCGCA TGC^GCA
KasI 193 GGCGCC G^GCGCC
KspI 252 CCGCGG CCGC^GG
NaeI 135 GCCGGC GCC^GGC
NarI 193 GGCGCC GG^CGCC
NcoI 2610 CCATGG C^CATGG
NdeI 6850 CATATG CA^TATG
NgoMIV 135 GCCGGC G^CCGGC
PshBI 6902 ATTAAT AT^TAAT
RcaI 855 TCATGA T^CATGA
SacII 252 CCGCGG CCGC^GG
SapI 5630 GAAGAGC GCTCTTC(1/4)
SbfI 7327 CCTGCAGG CCTGCA^GG
SexAI 2696 ACCTGGT A^CC[AT]GGT
SfoI 193 GGCGCC GGC^GCC
Sse8387I 7327 CCTGCAGG CCTGCA^GG
StuI 8435 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 235 GGTACC G^GTACC
8867 GGTACC
AcyI 193 GGCGCC G[GA]^CG[CT]C
302 GACGTC
AflII 5777 CTTAAG C^TTAAG
6284 CTTAAG
Asp718I 235 GGTACC G^GTACC
8867 GGTACC
AspI 1589 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6690 GACTTGGTC
BbrPI 2717 CACGTG CAC^GTG
4441 CACGTG
BcgI 4771 CGATCATTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
348 GCATTTACCTCG
BcnI 223 CCCGG CC^[GC]GG
8260 CCGGG
BfrI 5777 CTTAAG C^TTAAG
6284 CTTAAG
BglI 5814 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
7334 GCCAGGAAGGC
BglII 784 AGATCT A^GATCT
3550 AGATCT
BlpI 8120 GCTGAGC GC^T[ACGT]AGC
8643 GCTGAGC
Bpu1102I 8120 GCTGAGC GC^T[ACGT]AGC
8643 GCTGAGC
BsaBI 1312 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6940 GATTACCATC
BsaHI 193 GGCGCC G[GA]^CG[CT]C
302 GACGTC
Bsp1407I 2020 TGTACA T^GTACA
2058 TGTACA
BspDI 712 ATCGAT AT^CGAT
7443 ATCGAT
BsrGI 2020 TGTACA T^GTACA
2058 TGTACA
CelII 8120 GCTGAGC GC^T[ACGT]AGC
8643 GCTGAGC
ClaI 712 ATCGAT AT^CGAT
7443 ATCGAT
EcoT22I 1699 ATGCAT ATGCA^T
8538 ATGCAT
Hin1I 193 GGCGCC G[GA]^CG[CT]C
302 GACGTC
HpaI 3733 GTTAAC GTT^AAC
5202 GTTAAC
KpnI 235 GGTACC GGTAC^C
8867 GGTACC
MamI 1312 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6940 GATTACCATC
NciI 223 CCCGG CC^[GC]GG
8260 CCGGG
NheI 5447 GCTAGC G^CTAGC
8736 GCTAGC
NsiI 1699 ATGCAT ATGCA^T
8538 ATGCAT
PflFI 1589 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6690 GACTTGGTC
PmaCI 2717 CACGTG CAC^GTG
4441 CACGTG
PmlI 2717 CACGTG CAC^GTG
4441 CACGTG
SspBI 2020 TGTACA T^GTACA
2058 TGTACA
SwaI 1703 ATTTAAAT ATTT^AAAT
6915 ATTTAAAT
Tth111I 1589 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6690 GACTTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI ApaI ApoI Asp700I AspEI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbsI BbvCI BbvI
BciVI BfaI BlnI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspEI BspLU11I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DrdI DsaI EaeI Eam1105I
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshAI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SphI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XcmI
XhoII XmnI