##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
Acc65I Alw44I Aor51HI ApaLI AscI
Asp718I AviII BbeI BbrPI BsiEI
BsiWI BsrBI BstBI CpoI EagI
EclXI Eco47III Eco52I FseI FspI
KasI KpnI KspI MluI NaeI
NarI NgoMIV NotI NruI NspV
PacI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SbfI SfoI
SfuI SgrAI SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 9267 GACGTC GACGT^C
AccIII 1723 TCCGGA T^CCGGA
AclI 6664 AACGTT AA^CGTT
AgeI 9038 ACCGGT A^CCGGT
ApaI 7138 GGGCCC GGGCC^C
AseI 8896 ATTAAT AT^TAAT
AsnI 8896 ATTAAT AT^TAAT
AvrII 9284 CCTAGG C^CTAGG
BalI 7710 TGGCCA TGG^CCA
BciVI 2889 GTATCC GTATCC(6/5)
BlnI 9284 CCTAGG C^CTAGG
BsaAI 6016 TACGTG [CT]AC^GT[GA]
BseAI 1723 TCCGGA T^CCGGA
Bsp1407I 9347 TGTACA T^GTACA
BspEI 1723 TCCGGA T^CCGGA
BsrFI 9038 ACCGGT [GA]^CCGG[CT]
BsrGI 9347 TGTACA T^GTACA
Bst1107I 9338 GTATAC GTA^TAC
BstZ17I 9338 GTATAC GTA^TAC
Cfr10I 9038 ACCGGT [GA]^CCGG[CT]
DraI 6294 TTTAAA TTT^AAA
EcoRI 2802 GAATTC G^AATTC
EcoRV 2003 GATATC GAT^ATC
EcoT22I 4344 ATGCAT ATGCA^T
MfeI 7558 CAATTG C^AATTG
MluNI 7710 TGGCCA TGG^CCA
MroI 1723 TCCGGA T^CCGGA
MscI 7710 TGGCCA TGG^CCA
MunI 7558 CAATTG C^AATTG
NdeI 3064 CATATG CA^TATG
NsiI 4344 ATGCAT ATGCA^T
PinAI 9038 ACCGGT A^CCGGT
PshBI 8896 ATTAAT AT^TAAT
Psp1406I 6664 AACGTT AA^CGTT
ScaI 5546 AGTACT AGT^ACT
SfiI 2759 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 6996 CCCGGG CCC^GGG
SphI 1504 GCATGC GCATG^C
SspBI 9347 TGTACA T^GTACA
StuI 1627 AGGCCT AGG^CCT
XcmI 2977 CCAGAGGGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 6996 CCCGGG C^CCGGG
XmaI 6996 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 5370 GTCTAC GT^[AC][GT]AC
9338 GTATAC
AocI 7750 CCTTAGG CC^T[ACGT]AGG
7909 CCTGAGG
BamHI 1855 GGATCC G^GATCC
5429 GGATCC
BcgI 571 GCATTGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5823 GCAAATAAATCG
BglI 1794 GCCAATAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2760 GCCCAGGAGGC
BsaBI 6854 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
8473 GATTGACATC
BspDI 2006 ATCGAT AT^CGAT
8543 ATCGAT
BspLU11I 3005 ACATGT A^CATGT
8957 ACATGT
BssHII 834 GCGCGC G^CGCGC
1586 GCGCGC
BstAPI 2991 GCATCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
7121 GCACCTGGTGC
BstEII 3874 GGTCACC G^GT[ACGT]ACC
4425 GGTCACC
BstPI 3874 GGTCACC G^GT[ACGT]ACC
4425 GGTCACC
BstXI 1546 CCATGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3289 CCATGAAACTGG
Bsu36I 7750 CCTTAGG CC^T[ACGT]AGG
7909 CCTGAGG
ClaI 2006 ATCGAT AT^CGAT
8543 ATCGAT
EaeI 7710 TGGCCA [CT]^GGCC[GA]
8035 TGGCCG
Eco81I 7750 CCTTAGG CC^T[ACGT]AGG
7909 CCTGAGG
EcoNI 3546 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5648 CCTGGTCCAGG
EcoO65I 3874 GGTCACC G^GT[ACGT]ACC
4425 GGTCACC
HaeII 1047 GGCGCT [GA]GCGC^[CT]
2110 AGCGCC
MamI 6854 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
8473 GATTGACATC
PaeR7I 525 CTCGAG C^TCGAG
7674 CTCGAG
XhoI 525 CTCGAG C^TCGAG
7674 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AflIII AhdI AluI AlwI
AlwNI ApoI Asp700I AspEI AspHI
AspI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BclI BcnI
BfaI BfrI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI Eam1105I EarI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NheI
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PshAI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SpeI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XhoII XmnI