##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI AocI Aor51HI AscI
Asp718I AspEI AvrII BamHI BbeI
BglI BlnI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BssHII BssSI BstBI Bsu36I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I FseI HaeII
Hin1I HpaI KasI KpnI KspI
MluI MroI NaeI NarI NgoMIV
NotI NspV PaeR7I PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AccII 7882 CGCG CG^CG
ApaI 1087 GGGCCC GGGCC^C
BciVI 8564 GTATCC GTATCC(6/5)
BlpI 8873 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 8873 GCTTAGC GC^T[ACGT]AGC
BsrFI 4707 GCCGGT [GA]^CCGG[CT]
BstEII 8416 GGTGACC G^GT[ACGT]ACC
BstPI 8416 GGTGACC G^GT[ACGT]ACC
BstUI 7882 CGCG CG^CG
CelII 8873 GCTTAGC GC^T[ACGT]AGC
Cfr10I 4707 GCCGGT [GA]^CCGG[CT]
DrdI 900 GACTTACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 8251 CCTAACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 8416 GGTGACC G^GT[ACGT]ACC
HgaI 9845 GACGC GACGC(5/10)
MvnI 7882 CGCG CG^CG
NruI 7881 TCGCGA TCG^CGA
PacI 2282 TTAATTAA TTAAT^TAA
PflMI 911 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 37 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 2137 ACCAGGT A^CC[AT]GGT
SmaI 4464 CCCGGG CCC^GGG
TaqI 9609 TCGA T^CGA
TthHB8I 9609 TCGA T^CGA
Van91I 911 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 4464 CCCGGG C^CCGGG
XmaI 4464 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Asp700I 415 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5309 GAAAAGATTC
AspI 7859 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
9845 GACGCTGTC
AvaI 3664 CTCGGG C^[CT]CG[GA]G
4464 CCCGGG
AviII 807 TGCGCA TGC^GCA
7780 TGCGCA
BanI 96 GGTGCC G^G[CT][GA]CC
6721 GGTGCC
BbrPI 801 CACGTG CAC^GTG
3617 CACGTG
BcgI 7946 CGAAATGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3656 GCACTGAGCTCG
BclI 2157 TGATCA T^GATCA
9277 TGATCA
BcnI 4464 CCCGG CC^[GC]GG
4465 CCGGG
BsaBI 3987 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
8648 GATAGACATC
BsoBI 3664 CTCGGG C^[CT]CG[GA]G
4464 CCCGGG
Bst1107I 7302 GTATAC GTA^TAC
7440 GTATAC
BstZ17I 7302 GTATAC GTA^TAC
7440 GTATAC
DraIII 912 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1076 CACAATGTG
DsaI 1012 CCATGG C^C[GA][CT]GG
1753 CCATGG
EcoRV 3991 GATATC GAT^ATC
4324 GATATC
FauI 240 CCCGC CCCGC(4/6)
9315 CCCGC
FbaI 2157 TGATCA T^GATCA
9277 TGATCA
FspI 807 TGCGCA TGC^GCA
7780 TGCGCA
HindIII 9130 AAGCTT A^AGCTT
10127 AAGCTT
MamI 3987 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
8648 GATAGACATC
MfeI 7842 CAATTG C^AATTG
9368 CAATTG
MunI 7842 CAATTG C^AATTG
9368 CAATTG
NciI 4464 CCCGG CC^[GC]GG
4465 CCGGG
NcoI 1012 CCATGG C^CATGG
1753 CCATGG
NdeI 229 CATATG CA^TATG
3493 CATATG
NheI 7693 GCTAGC G^CTAGC
9095 GCTAGC
PflFI 7859 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
9845 GACGCTGTC
PmaCI 801 CACGTG CAC^GTG
3617 CACGTG
PmlI 801 CACGTG CAC^GTG
3617 CACGTG
PstI 4699 CTGCAG CTGCA^G
7171 CTGCAG
ScaI 6436 AGTACT AGT^ACT
8921 AGTACT
Tth111I 7859 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
9845 GACGCTGTC
XcmI 367 CCAGTTATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
9924 CCATTTATATTTTGG
XmnI 415 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5309 GAAAAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI Alw44I AlwI AlwNI
ApaLI ApoI AseI AsnI AspHI
AvaII BalI BanII BbsI BbvCI
BbvI BfaI BfrI BglII BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspHI BspLU11I BspMI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI PshBI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SpeI SphI
SspBI SspI StuI StyI SwaI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XbaI XhoII