##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AscI AseI AsnI Asp718I AspEI
BbrPI BsaBI BsiEI BsiWI BsmBI
BssHII BstBI CpoI DraI EagI
Eam1105I EclXI Eco52I EcoRV FseI
KpnI MamI NaeI NdeI NgoMIV
NheI NotI NruI NspV PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SalI SfuI SgrAI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 23 enzymes) #
###################################
ApaI 4383 GGGCCC GGGCC^C
BamHI 4684 GGATCC G^GATCC
BbeI 5616 GGCGCC GGCGC^C
BclI 3625 TGATCA T^GATCA
BsaAI 1759 TACGTA [CT]AC^GT[GA]
BspDI 6779 ATCGAT AT^CGAT
Bst1107I 531 GTATAC GTA^TAC
BstZ17I 531 GTATAC GTA^TAC
ClaI 6779 ATCGAT AT^CGAT
FbaI 3625 TGATCA T^GATCA
HindIII 5355 AAGCTT A^AGCTT
HpaI 9200 GTTAAC GTT^AAC
KasI 5616 GGCGCC G^GCGCC
MfeI 6392 CAATTG C^AATTG
MluI 1056 ACGCGT A^CGCGT
MunI 6392 CAATTG C^AATTG
NarI 5616 GGCGCC GG^CGCC
PacI 8877 TTAATTAA TTAAT^TAA
SfiI 4045 GGCCATGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 5616 GGCGCC GGC^GCC
SnaBI 1759 TACGTA TAC^GTA
SpeI 2111 ACTAGT A^CTAGT
XbaI 3247 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 531 GTATAC GT^[AC][GT]AC
7915 GTAGAC
AspI 5371 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6422 GACGTGGTC
AviII 5865 TGCGCA TGC^GCA
7973 TGCGCA
BcgI 1888 CGAGGACTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2137 CGATGAGTTTGC
BglII 6999 AGATCT A^GATCT
8358 AGATCT
BspHI 6033 TCATGA T^CATGA
6561 TCATGA
BspLU11I 7532 ACATGT A^CATGT
9279 ACATGT
BsrFI 2370 ACCGGC [GA]^CCGG[CT]
2547 GCCGGT
BstEII 5951 GGTCACC G^GT[ACGT]ACC
7162 GGTCACC
BstPI 5951 GGTCACC G^GT[ACGT]ACC
7162 GGTCACC
Cfr10I 2370 ACCGGC [GA]^CCGG[CT]
2547 GCCGGT
DrdI 3134 GACCAGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4598 GACATGGCTGTC
EcoO65I 5951 GGTCACC G^GT[ACGT]ACC
7162 GGTCACC
EcoRI 1184 GAATTC G^AATTC
2773 GAATTC
EcoT22I 7 ATGCAT ATGCA^T
4509 ATGCAT
FspI 5865 TGCGCA TGC^GCA
7973 TGCGCA
KspI 2847 CCGCGG CCGC^GG
5403 CCGCGG
NsiI 7 ATGCAT ATGCA^T
4509 ATGCAT
PflFI 5371 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6422 GACGTGGTC
RcaI 6033 TCATGA T^CATGA
6561 TCATGA
SacII 2847 CCGCGG CCGC^GG
5403 CCGCGG
SbfI 849 CCTGCAGG CCTGCA^GG
871 CCTGCAGG
SexAI 3135 ACCAGGT A^CC[AT]GGT
9195 ACCAGGT
SmaI 746 CCCGGG CCC^GGG
2427 CCCGGG
Sse8387I 849 CCTGCAGG CCTGCA^GG
871 CCTGCAGG
Tth111I 5371 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6422 GACGTGGTC
XmaCI 746 CCCGGG C^CCGGG
2427 CCCGGG
XmaI 746 CCCGGG C^CCGGG
2427 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 160 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AflII AflIII AluI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI Asp700I AspHI AvaI AvaII
AvrII BalI BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BfrI BglI BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspEI
BspMI BsrBI BsrDI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PaeR7I PflMI PleI PpuMI
PshAI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SphI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoI XhoII XmnI