##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BbeI BbrPI BbvCI
BcgI BclI BfrI BglII BsaAI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BspLU11I
BsrBI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 2492 GTCTAC GT^[AC][GT]AC
AcsI 2854 AAATTT [GA]^AATT[CT]
AgeI 1746 ACCGGT A^CCGGT
Alw44I 2055 GTGCAC G^TGCAC
AocI 1870 CCTTAGG CC^T[ACGT]AGG
ApaI 2139 GGGCCC GGGCC^C
ApaLI 2055 GTGCAC G^TGCAC
ApoI 2854 AAATTT [GA]^AATT[CT]
AvrII 2636 CCTAGG C^CTAGG
BamHI 2044 GGATCC G^GATCC
BanI 1783 GGTGCC G^G[CT][GA]CC
BbsI 789 GTCTTC GAAGAC(2/6)
BlnI 2636 CCTAGG C^CTAGG
BlpI 1637 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1637 GCTGAGC GC^T[ACGT]AGC
BpuAI 789 GTCTTC GAAGAC(2/6)
BsaI 628 GAGACC GGTCTC(1/5)
BsaWI 1746 ACCGGT [AT]^CCGG[AT]
BsmI 204 GAATGC GAATGC(1/-1)
Bsp1407I 1901 TGTACA T^GTACA
BsrFI 1746 ACCGGT [GA]^CCGG[CT]
BsrGI 1901 TGTACA T^GTACA
BssSI 1467 CACGAG CACGAG(-5/-1)
BstXI 181 CCAAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1870 CCTTAGG CC^T[ACGT]AGG
CelII 1637 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1746 ACCGGT [GA]^CCGG[CT]
DraI 2397 TTTAAA TTT^AAA
DraIII 749 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1680 GACCAGCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1934 CCATGG C^C[GA][CT]GG
Eco81I 1870 CCTTAGG CC^T[ACGT]AGG
EcoT22I 153 ATGCAT ATGCA^T
HindIII 1734 AAGCTT A^AGCTT
MaeII 2905 ACGT A^CGT
NcoI 1934 CCATGG C^CATGG
NdeI 1265 CATATG CA^TATG
NheI 2631 GCTAGC G^CTAGC
NsiI 153 ATGCAT ATGCA^T
PflMI 978 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1746 ACCGGT A^CCGGT
ScaI 1516 AGTACT AGT^ACT
SspBI 1901 TGTACA T^GTACA
StuI 48 AGGCCT AGG^CCT
Van91I 978 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 277 CCAAGCCAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Aor51HI 95 AGCGCT AGC^GCT
1613 AGCGCT
AvaI 783 CTCGGG C^[CT]CG[GA]G
2452 CTCGGG
BalI 664 TGGCCA TGG^CCA
1958 TGGCCA
BciVI 608 GGATAC GTATCC(6/5)
161 GTATCC
BglI 1474 GCCGGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2308 GCCATGCTGGC
BseRI 774 CTCCTC GAGGAG(10/8)
2076 GAGGAG
BsoBI 783 CTCGGG C^[CT]CG[GA]G
2452 CTCGGG
BspMI 2304 ACCTGC ACCTGC(4/8)
147 GCAGGT
BsrDI 588 CATTGC GCAATG(2/0)
991 GCAATG
BstAPI 1772 GCAGTTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2622 GCAGCTCATGC
BstYI 343 AGATCC [GA]^GATC[CT]
2044 GGATCC
CfoI 96 GCGC GCG^C
1614 GCGC
EaeI 664 TGGCCA [CT]^GGCC[GA]
1958 TGGCCA
Eco47III 95 AGCGCT AGC^GCT
1613 AGCGCT
HaeII 95 AGCGCT [GA]GCGC^[CT]
1613 AGCGCT
HhaI 96 GCGC GCG^C
1614 GCGC
HinP1I 96 GCGC G^CGC
1614 GCGC
MflI 343 AGATCC [GA]^GATC[CT]
2044 GGATCC
MluNI 664 TGGCCA TGG^CCA
1958 TGGCCA
MscI 664 TGGCCA TGG^CCA
1958 TGGCCA
NspI 123 GCATGT [GA]CATG^[CT]
1225 ACATGC
SbfI 1363 CCTGCAGG CCTGCA^GG
1697 CCTGCAGG
Sse8387I 1363 CCTGCAGG CCTGCA^GG
1697 CCTGCAGG
XhoII 343 AGATCC [GA]^GATC[CT]
2044 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I