################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII AccIII AclI AgeI Alw44I ApaLI AscI BbeI BseAI BsiEI BsmBI BspDI BspEI BspHI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI EagI EclXI Eco52I FseI HpaI KasI MluI MroI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RcaI RsrII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 5750 GGTACC G^GTACC AcyI 8500 GACGCC G[GA]^CG[CT]C AflII 736 CTTAAG C^TTAAG Aor51HI 2193 AGCGCT AGC^GCT AseI 791 ATTAAT AT^TAAT AsnI 791 ATTAAT AT^TAAT Asp718I 5750 GGTACC G^GTACC AspI 6400 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 948 TGCGCA TGC^GCA BamHI 7241 GGATCC G^GATCC BcgI 4943 CGATATGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 5978 GGATAC GTATCC(6/5) BclI 5841 TGATCA T^GATCA BfrI 736 CTTAAG C^TTAAG BglI 6891 GCCTCTTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 8500 GACGCC G[GA]^CG[CT]C BsaWI 7726 TCCGGT [AT]^CCGG[AT] BsiWI 8225 CGTACG C^GTACG DraIII 3170 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 2193 AGCGCT AGC^GCT EcoRV 4734 GATATC GAT^ATC EcoT22I 5519 ATGCAT ATGCA^T FbaI 5841 TGATCA T^GATCA FspI 948 TGCGCA TGC^GCA HaeII 2193 AGCGCT [GA]GCGC^[CT] Hin1I 8500 GACGCC G[GA]^CG[CT]C KpnI 5750 GGTACC GGTAC^C MfeI 2343 CAATTG C^AATTG MunI 2343 CAATTG C^AATTG NheI 2470 GCTAGC G^CTAGC NsiI 5519 ATGCAT ATGCA^T PflFI 6400 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 8488 GACTACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 791 ATTAAT AT^TAAT SapI 2720 GAAGAGC GCTCTTC(1/4) ScaI 2738 AGTACT AGT^ACT SmaI 2063 CCCGGG CCC^GGG SpeI 4387 ACTAGT A^CTAGT SspI 4963 AATATT AAT^ATT Tth111I 6400 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 2063 CCCGGG C^CCGGG XmaI 2063 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AhdI 2597 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3289 GACTGTAGGTC ApaI 2759 GGGCCC GGGCC^C 4572 GGGCCC AspEI 2597 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3289 GACTGTAGGTC BbrPI 6597 CACGTG CAC^GTG 8322 CACGTG BcnI 2063 CCCGG CC^[GC]GG 2064 CCGGG BsaBI 5469 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 7953 GATTCACATC BsrBI 6279 GAGCGG CCGCTC(-3/-3) 7480 GAGCGG BsrFI 7403 ACCGGC [GA]^CCGG[CT] 8964 GCCGGT BstEII 5560 GGTAACC G^GT[ACGT]ACC 8768 GGTGACC BstPI 5560 GGTAACC G^GT[ACGT]ACC 8768 GGTGACC Cfr10I 7403 ACCGGC [GA]^CCGG[CT] 8964 GCCGGT Eam1105I 2597 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3289 GACTGTAGGTC EcoO65I 5560 GGTAACC G^GT[ACGT]ACC 8768 GGTGACC HincII 179 GTTGAC GT[CT]^[GA]AC 7554 GTCAAC HindII 179 GTTGAC GT[CT]^[GA]AC 7554 GTCAAC KspI 6251 CCGCGG CCGC^GG 8717 CCGCGG MamI 5469 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 7953 GATTCACATC NciI 2063 CCCGG CC^[GC]GG 2064 CCGGG PmaCI 6597 CACGTG CAC^GTG 8322 CACGTG PmlI 6597 CACGTG CAC^GTG 8322 CACGTG SacII 6251 CCGCGG CCGC^GG 8717 CCGCGG StuI 8417 AGGCCT AGG^CCT 8606 AGGCCT XbaI 861 TCTAGA T^CTAGA 8710 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 139 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII AvrII BalI BanI BanII BbsI BbvCI BbvI BfaI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrDI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI