##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
ApaLI AscI BbeI BseAI BsiEI
BsmBI BspDI BspEI BspHI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco52I FseI
HpaI KasI MluI MroI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmeI Psp1406I
PvuI RcaI RsrII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 5750 GGTACC G^GTACC
AcyI 8500 GACGCC G[GA]^CG[CT]C
AflII 736 CTTAAG C^TTAAG
Aor51HI 2193 AGCGCT AGC^GCT
AseI 791 ATTAAT AT^TAAT
AsnI 791 ATTAAT AT^TAAT
Asp718I 5750 GGTACC G^GTACC
AspI 6400 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 948 TGCGCA TGC^GCA
BamHI 7241 GGATCC G^GATCC
BcgI 4943 CGATATGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5978 GGATAC GTATCC(6/5)
BclI 5841 TGATCA T^GATCA
BfrI 736 CTTAAG C^TTAAG
BglI 6891 GCCTCTTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 8500 GACGCC G[GA]^CG[CT]C
BsaWI 7726 TCCGGT [AT]^CCGG[AT]
BsiWI 8225 CGTACG C^GTACG
DraIII 3170 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2193 AGCGCT AGC^GCT
EcoRV 4734 GATATC GAT^ATC
EcoT22I 5519 ATGCAT ATGCA^T
FbaI 5841 TGATCA T^GATCA
FspI 948 TGCGCA TGC^GCA
HaeII 2193 AGCGCT [GA]GCGC^[CT]
Hin1I 8500 GACGCC G[GA]^CG[CT]C
KpnI 5750 GGTACC GGTAC^C
MfeI 2343 CAATTG C^AATTG
MunI 2343 CAATTG C^AATTG
NheI 2470 GCTAGC G^CTAGC
NsiI 5519 ATGCAT ATGCA^T
PflFI 6400 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 8488 GACTACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 791 ATTAAT AT^TAAT
SapI 2720 GAAGAGC GCTCTTC(1/4)
ScaI 2738 AGTACT AGT^ACT
SmaI 2063 CCCGGG CCC^GGG
SpeI 4387 ACTAGT A^CTAGT
SspI 4963 AATATT AAT^ATT
Tth111I 6400 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2063 CCCGGG C^CCGGG
XmaI 2063 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AhdI 2597 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3289 GACTGTAGGTC
ApaI 2759 GGGCCC GGGCC^C
4572 GGGCCC
AspEI 2597 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3289 GACTGTAGGTC
BbrPI 6597 CACGTG CAC^GTG
8322 CACGTG
BcnI 2063 CCCGG CC^[GC]GG
2064 CCGGG
BsaBI 5469 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7953 GATTCACATC
BsrBI 6279 GAGCGG CCGCTC(-3/-3)
7480 GAGCGG
BsrFI 7403 ACCGGC [GA]^CCGG[CT]
8964 GCCGGT
BstEII 5560 GGTAACC G^GT[ACGT]ACC
8768 GGTGACC
BstPI 5560 GGTAACC G^GT[ACGT]ACC
8768 GGTGACC
Cfr10I 7403 ACCGGC [GA]^CCGG[CT]
8964 GCCGGT
Eam1105I 2597 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3289 GACTGTAGGTC
EcoO65I 5560 GGTAACC G^GT[ACGT]ACC
8768 GGTGACC
HincII 179 GTTGAC GT[CT]^[GA]AC
7554 GTCAAC
HindII 179 GTTGAC GT[CT]^[GA]AC
7554 GTCAAC
KspI 6251 CCGCGG CCGC^GG
8717 CCGCGG
MamI 5469 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7953 GATTCACATC
NciI 2063 CCCGG CC^[GC]GG
2064 CCGGG
PmaCI 6597 CACGTG CAC^GTG
8322 CACGTG
PmlI 6597 CACGTG CAC^GTG
8322 CACGTG
SacII 6251 CCGCGG CCGC^GG
8717 CCGCGG
StuI 8417 AGGCCT AGG^CCT
8606 AGGCCT
XbaI 861 TCTAGA T^CTAGA
8710 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AvaI AvaII
AvrII BalI BanI BanII BbsI
BbvCI BbvI BfaI BglII BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspLU11I
BspMI BsrDI BsrGI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NcoI NdeI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI