##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BcnI BglI
BglII BlnI BlpI Bpu1102I BsaI
BseAI BsiWI BsoBI BspDI BspEI
BssHII BstBI BstEII BstPI BstUI
BstXI CelII CfoI ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I FseI FspI HaeII HhaI
HinP1I KasI KpnI KspI MluI
MroI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PaeR7I PinAI PmaCI PmlI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 4844 GGCGTC G[GA]^CG[CT]C
AflII 3950 CTTAAG C^TTAAG
Asp700I 874 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 5691 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2055 TGGCCA TGG^CCA
BanI 5360 GGCACC G^G[CT][GA]CC
BfrI 3950 CTTAAG C^TTAAG
BsaBI 823 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4844 GGCGTC G[GA]^CG[CT]C
BsaWI 3910 ACCGGA [AT]^CCGG[AT]
BsiEI 586 CGACCG CG[GA][CT]^CG
BsrBI 602 GAGCGG CCGCTC(-3/-3)
BssSI 1922 CACGAG CACGAG(-5/-1)
Bst1107I 5936 GTATAC GTA^TAC
BstAPI 1289 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 5936 GTATAC GTA^TAC
EaeI 2055 TGGCCA [CT]^GGCC[GA]
EcoNI 2810 CCTTTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2346 ATGCAT ATGCA^T
Hin1I 4844 GGCGTC G[GA]^CG[CT]C
MamI 823 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2055 TGGCCA TGG^CCA
MscI 2055 TGGCCA TGG^CCA
NcoI 3707 CCATGG C^CATGG
NsiI 2346 ATGCAT ATGCA^T
PacI 770 TTAATTAA TTAAT^TAA
PflFI 5691 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 5870 GTTTAAAC GTTT^AAAC
PshAI 1699 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3047 GAGCTC GAGCT^C
SphI 2161 GCATGC GCATG^C
Tth111I 5691 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4423 TCTAGA T^CTAGA
XmnI 874 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AseI 4372 ATTAAT AT^TAAT
5664 ATTAAT
AsnI 4372 ATTAAT AT^TAAT
5664 ATTAAT
BsmBI 1238 GAGACG CGTCTC(1/5)
3568 GAGACG
BsmI 694 GAATGC GAATGC(1/-1)
2291 GAATGC
Bsp1407I 1066 TGTACA T^GTACA
2430 TGTACA
BspLU11I 944 ACATGT A^CATGT
3818 ACATGT
BsrDI 5656 CATTGC GCAATG(2/0)
84 GCAATG
BsrFI 588 ACCGGC [GA]^CCGG[CT]
1638 ACCGGC
BsrGI 1066 TGTACA T^GTACA
2430 TGTACA
Cfr10I 588 ACCGGC [GA]^CCGG[CT]
1638 ACCGGC
DraIII 2434 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3739 CACACAGTG
DrdI 1240 GACGTTTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3756 GACTCCAGAGTC
DsaI 3707 CCATGG C^C[GA][CT]GG
5364 CCGTGG
EcoRI 3985 GAATTC G^AATTC
4243 GAATTC
EcoRV 206 GATATC GAT^ATC
532 GATATC
EcoT14I 3653 CCTTGG C^C[AT][AT]GG
3707 CCATGG
FauI 2028 CCCGC CCCGC(4/6)
5749 CCCGC
HgaI 3411 GACGC GACGC(5/10)
4845 GCGTC
HincII 5061 GTTAAC GT[CT]^[GA]AC
5113 GTTAAC
HindII 5061 GTTAAC GT[CT]^[GA]AC
5113 GTTAAC
HindIII 1664 AAGCTT A^AGCTT
3602 AAGCTT
HpaI 5061 GTTAAC GTT^AAC
5113 GTTAAC
MfeI 4023 CAATTG C^AATTG
5056 CAATTG
MunI 4023 CAATTG C^AATTG
5056 CAATTG
PflMI 845 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3677 CCAAAGCGTGG
PshBI 4372 ATTAAT AT^TAAT
5664 ATTAAT
SapI 3140 GAAGAGC GCTCTTC(1/4)
1556 GCTCTTC
SnaBI 447 TACGTA TAC^GTA
3959 TACGTA
SspBI 1066 TGTACA T^GTACA
2430 TGTACA
StyI 3653 CCTTGG C^C[AT][AT]GG
3707 CCATGG
Van91I 845 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3677 CCAAAGCGTGG
XcmI 459 CCATGATGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2478 CCACGTACAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BanII BbsI BbvI
BclI BfaI BmyI BpmI BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BspMI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
Eco81I EcoO109I EcoRII FbaI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StuI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII