################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII AclI AgeI Alw44I Aor51HI ApaLI AscI AspHI AvaI AviII BalI BbrPI BcgI BglI BlpI Bpu1102I BsiEI BsiHKAI BsiWI BsmBI BsoBI BspDI BsrBI BssHII CelII ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI KspI MluI MluNI MscI NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SapI SbfI SexAI SfiI SgrAI SmaI SnaBI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccIII 2889 TCCGGA T^CCGGA ApaI 1483 GGGCCC GGGCC^C AspI 288 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3075 CCTAGG C^CTAGG BamHI 722 GGATCC G^GATCC BbeI 18 GGCGCC GGCGC^C BbsI 835 GTCTTC GAAGAC(2/6) BbvCI 2044 GCTGAGG CCTCAGC(-5/-2) BglII 4014 AGATCT A^GATCT BlnI 3075 CCTAGG C^CTAGG BpuAI 835 GTCTTC GAAGAC(2/6) BsaAI 2511 CACGTA [CT]AC^GT[GA] BsaBI 3366 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2889 TCCGGA [AT]^CCGG[AT] BseAI 2889 TCCGGA T^CCGGA BsmI 1844 GAATGC GAATGC(1/-1) BspEI 2889 TCCGGA T^CCGGA BspMI 2302 ACCTGC ACCTGC(4/8) BsrFI 3462 GCCGGC [GA]^CCGG[CT] BssSI 4332 CTCGTG CACGAG(-5/-1) BstBI 440 TTCGAA TT^CGAA BstEII 3987 GGTAACC G^GT[ACGT]ACC BstPI 3987 GGTAACC G^GT[ACGT]ACC Cfr10I 3462 GCCGGC [GA]^CCGG[CT] DraII 2492 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO109I 2492 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 3987 GGTAACC G^GT[ACGT]ACC HaeII 18 GGCGCC [GA]GCGC^[CT] HindIII 2139 AAGCTT A^AGCTT HpaI 759 GTTAAC GTT^AAC KasI 18 GGCGCC G^GCGCC MamI 3366 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2889 TCCGGA T^CCGGA NaeI 3462 GCCGGC GCC^GGC NarI 18 GGCGCC GG^CGCC NdeI 4611 CATATG CA^TATG NgoMIV 3462 GCCGGC G^CCGGC NspV 440 TTCGAA TT^CGAA PflFI 288 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 2295 CTGCAG CTGCA^G SalI 4077 GTCGAC G^TCGAC SfoI 18 GGCGCC GGC^GCC SfuI 440 TTCGAA TT^CGAA SpeI 3325 ACTAGT A^CTAGT StuI 5117 AGGCCT AGG^CCT Tth111I 288 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 4254 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 2432 GGTACC G^GTACC 2864 GGTACC AflII 3278 CTTAAG C^TTAAG 5305 CTTAAG AhdI 2268 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4549 GACCGTAAGTC Asp700I 1989 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3182 GAACTTATTC Asp718I 2432 GGTACC G^GTACC 2864 GGTACC AspEI 2268 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4549 GACCGTAAGTC BciVI 589 GTATCC GTATCC(6/5) 4933 GTATCC BfrI 3278 CTTAAG C^TTAAG 5305 CTTAAG BpmI 1701 CTCCAG CTGGAG(16/14) 5081 CTGGAG BspHI 2453 TCATGA T^CATGA 3170 TCATGA Bst1107I 3561 GTATAC GTA^TAC 3629 GTATAC BstAPI 2297 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4946 GCAAGAGATGC BstZ17I 3561 GTATAC GTA^TAC 3629 GTATAC EaeI 2615 TGGCCG [CT]^GGCC[GA] 3457 TGGCCG Eam1105I 2268 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4549 GACCGTAAGTC EarI 3080 GAAGAG CTCTTC(1/4) 4636 GAAGAG EcoNI 4154 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4465 CCTGCCTTAGG EcoRI 479 GAATTC G^AATTC 3123 GAATTC KpnI 2432 GGTACC GGTAC^C 2864 GGTACC Ksp632I 3080 GAAGAG CTCTTC(1/4) 4636 GAAGAG MfeI 622 CAATTG C^AATTG 1678 CAATTG MunI 622 CAATTG C^AATTG 1678 CAATTG RcaI 2453 TCATGA T^CATGA 3170 TCATGA SphI 109 GCATGC GCATG^C 2984 GCATGC XcmI 221 CCACTATAACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1487 CCATATTTTAAGTGG XmnI 1989 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3182 GAACTTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApoI AseI AsnI AvaII BanI BanII BbvI BclI BcnI BfaI BmyI BsaHI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraIII DsaI Eco81I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PshBI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII