##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AclI AgeI Alw44I Aor51HI
ApaLI AscI AspHI AvaI AviII
BalI BbrPI BcgI BglI BlpI
Bpu1102I BsiEI BsiHKAI BsiWI BsmBI
BsoBI BspDI BsrBI BssHII CelII
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
FspI KspI MluI MluNI MscI
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacI SacII SapI SbfI SexAI
SfiI SgrAI SmaI SnaBI Sse8387I
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 2889 TCCGGA T^CCGGA
ApaI 1483 GGGCCC GGGCC^C
AspI 288 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3075 CCTAGG C^CTAGG
BamHI 722 GGATCC G^GATCC
BbeI 18 GGCGCC GGCGC^C
BbsI 835 GTCTTC GAAGAC(2/6)
BbvCI 2044 GCTGAGG CCTCAGC(-5/-2)
BglII 4014 AGATCT A^GATCT
BlnI 3075 CCTAGG C^CTAGG
BpuAI 835 GTCTTC GAAGAC(2/6)
BsaAI 2511 CACGTA [CT]AC^GT[GA]
BsaBI 3366 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2889 TCCGGA [AT]^CCGG[AT]
BseAI 2889 TCCGGA T^CCGGA
BsmI 1844 GAATGC GAATGC(1/-1)
BspEI 2889 TCCGGA T^CCGGA
BspMI 2302 ACCTGC ACCTGC(4/8)
BsrFI 3462 GCCGGC [GA]^CCGG[CT]
BssSI 4332 CTCGTG CACGAG(-5/-1)
BstBI 440 TTCGAA TT^CGAA
BstEII 3987 GGTAACC G^GT[ACGT]ACC
BstPI 3987 GGTAACC G^GT[ACGT]ACC
Cfr10I 3462 GCCGGC [GA]^CCGG[CT]
DraII 2492 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 2492 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3987 GGTAACC G^GT[ACGT]ACC
HaeII 18 GGCGCC [GA]GCGC^[CT]
HindIII 2139 AAGCTT A^AGCTT
HpaI 759 GTTAAC GTT^AAC
KasI 18 GGCGCC G^GCGCC
MamI 3366 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2889 TCCGGA T^CCGGA
NaeI 3462 GCCGGC GCC^GGC
NarI 18 GGCGCC GG^CGCC
NdeI 4611 CATATG CA^TATG
NgoMIV 3462 GCCGGC G^CCGGC
NspV 440 TTCGAA TT^CGAA
PflFI 288 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 2295 CTGCAG CTGCA^G
SalI 4077 GTCGAC G^TCGAC
SfoI 18 GGCGCC GGC^GCC
SfuI 440 TTCGAA TT^CGAA
SpeI 3325 ACTAGT A^CTAGT
StuI 5117 AGGCCT AGG^CCT
Tth111I 288 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4254 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 2432 GGTACC G^GTACC
2864 GGTACC
AflII 3278 CTTAAG C^TTAAG
5305 CTTAAG
AhdI 2268 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4549 GACCGTAAGTC
Asp700I 1989 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3182 GAACTTATTC
Asp718I 2432 GGTACC G^GTACC
2864 GGTACC
AspEI 2268 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4549 GACCGTAAGTC
BciVI 589 GTATCC GTATCC(6/5)
4933 GTATCC
BfrI 3278 CTTAAG C^TTAAG
5305 CTTAAG
BpmI 1701 CTCCAG CTGGAG(16/14)
5081 CTGGAG
BspHI 2453 TCATGA T^CATGA
3170 TCATGA
Bst1107I 3561 GTATAC GTA^TAC
3629 GTATAC
BstAPI 2297 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4946 GCAAGAGATGC
BstZ17I 3561 GTATAC GTA^TAC
3629 GTATAC
EaeI 2615 TGGCCG [CT]^GGCC[GA]
3457 TGGCCG
Eam1105I 2268 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4549 GACCGTAAGTC
EarI 3080 GAAGAG CTCTTC(1/4)
4636 GAAGAG
EcoNI 4154 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4465 CCTGCCTTAGG
EcoRI 479 GAATTC G^AATTC
3123 GAATTC
KpnI 2432 GGTACC GGTAC^C
2864 GGTACC
Ksp632I 3080 GAAGAG CTCTTC(1/4)
4636 GAAGAG
MfeI 622 CAATTG C^AATTG
1678 CAATTG
MunI 622 CAATTG C^AATTG
1678 CAATTG
RcaI 2453 TCATGA T^CATGA
3170 TCATGA
SphI 109 GCATGC GCATG^C
2984 GCATGC
XcmI 221 CCACTATAACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1487 CCATATTTTAAGTGG
XmnI 1989 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3182 GAACTTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI AseI AsnI AvaII
BanI BanII BbvI BclI BcnI
BfaI BmyI BsaHI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspLU11I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraIII DsaI Eco81I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PshBI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII