##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       AclI        AgeI        Alw44I      Aor51HI     
ApaLI       AscI        AspHI       AvaI        AviII       
BalI        BbrPI       BcgI        BglI        BlpI        
Bpu1102I    BsiEI       BsiHKAI     BsiWI       BsmBI       
BsoBI       BspDI       BsrBI       BssHII      CelII       
ClaI        CpoI        DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoRV       EcoT22I     FseI        
FspI        KspI        MluI        MluNI       MscI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        RsrII       
SacI        SacII       SapI        SbfI        SexAI       
SfiI        SgrAI       SmaI        SnaBI       Sse8387I    
SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccIII       2889  TCCGGA               T^CCGGA
ApaI         1483  GGGCCC               GGGCC^C
AspI          288  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        3075  CCTAGG               C^CTAGG
BamHI         722  GGATCC               G^GATCC
BbeI           18  GGCGCC               GGCGC^C
BbsI          835  GTCTTC               GAAGAC(2/6)
BbvCI        2044  GCTGAGG              CCTCAGC(-5/-2)
BglII        4014  AGATCT               A^GATCT
BlnI         3075  CCTAGG               C^CTAGG
BpuAI         835  GTCTTC               GAAGAC(2/6)
BsaAI        2511  CACGTA               [CT]AC^GT[GA]
BsaBI        3366  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2889  TCCGGA               [AT]^CCGG[AT]
BseAI        2889  TCCGGA               T^CCGGA
BsmI         1844  GAATGC               GAATGC(1/-1)
BspEI        2889  TCCGGA               T^CCGGA
BspMI        2302  ACCTGC               ACCTGC(4/8)
BsrFI        3462  GCCGGC               [GA]^CCGG[CT]
BssSI        4332  CTCGTG               CACGAG(-5/-1)
BstBI         440  TTCGAA               TT^CGAA
BstEII       3987  GGTAACC              G^GT[ACGT]ACC
BstPI        3987  GGTAACC              G^GT[ACGT]ACC
Cfr10I       3462  GCCGGC               [GA]^CCGG[CT]
DraII        2492  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I     2492  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      3987  GGTAACC              G^GT[ACGT]ACC
HaeII          18  GGCGCC               [GA]GCGC^[CT]
HindIII      2139  AAGCTT               A^AGCTT
HpaI          759  GTTAAC               GTT^AAC
KasI           18  GGCGCC               G^GCGCC
MamI         3366  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2889  TCCGGA               T^CCGGA
NaeI         3462  GCCGGC               GCC^GGC
NarI           18  GGCGCC               GG^CGCC
NdeI         4611  CATATG               CA^TATG
NgoMIV       3462  GCCGGC               G^CCGGC
NspV          440  TTCGAA               TT^CGAA
PflFI         288  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI         2295  CTGCAG               CTGCA^G
SalI         4077  GTCGAC               G^TCGAC
SfoI           18  GGCGCC               GGC^GCC
SfuI          440  TTCGAA               TT^CGAA
SpeI         3325  ACTAGT               A^CTAGT
StuI         5117  AGGCCT               AGG^CCT
Tth111I       288  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         4254  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Acc65I       2432  GGTACC               G^GTACC
             2864  GGTACC              
AflII        3278  CTTAAG               C^TTAAG
             5305  CTTAAG              
AhdI         2268  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4549  GACCGTAAGTC         
Asp700I      1989  GAATCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3182  GAACTTATTC          
Asp718I      2432  GGTACC               G^GTACC
             2864  GGTACC              
AspEI        2268  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4549  GACCGTAAGTC         
BciVI         589  GTATCC               GTATCC(6/5)
             4933  GTATCC              
BfrI         3278  CTTAAG               C^TTAAG
             5305  CTTAAG              
BpmI         1701  CTCCAG               CTGGAG(16/14)
             5081  CTGGAG              
BspHI        2453  TCATGA               T^CATGA
             3170  TCATGA              
Bst1107I     3561  GTATAC               GTA^TAC
             3629  GTATAC              
BstAPI       2297  GCAGTACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4946  GCAAGAGATGC         
BstZ17I      3561  GTATAC               GTA^TAC
             3629  GTATAC              
EaeI         2615  TGGCCG               [CT]^GGCC[GA]
             3457  TGGCCG              
Eam1105I     2268  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4549  GACCGTAAGTC         
EarI         3080  GAAGAG               CTCTTC(1/4)
             4636  GAAGAG              
EcoNI        4154  CCTGTGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4465  CCTGCCTTAGG         
EcoRI         479  GAATTC               G^AATTC
             3123  GAATTC              
KpnI         2432  GGTACC               GGTAC^C
             2864  GGTACC              
Ksp632I      3080  GAAGAG               CTCTTC(1/4)
             4636  GAAGAG              
MfeI          622  CAATTG               C^AATTG
             1678  CAATTG              
MunI          622  CAATTG               C^AATTG
             1678  CAATTG              
RcaI         2453  TCATGA               T^CATGA
             3170  TCATGA              
SphI          109  GCATGC               GCATG^C
             2984  GCATGC              
XcmI          221  CCACTATAACCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1487  CCATATTTTAAGTGG     
XmnI         1989  GAATCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3182  GAACTTATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
AocI        ApoI        AseI        AsnI        AvaII       
BanI        BanII       BbvI        BclI        BcnI        
BfaI        BmyI        BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    Bsp1407I    BspLU11I    BsrDI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraIII      DsaI        Eco81I      EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PshBI       PvuII       
RsaI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SmlI        SspBI       SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII