################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BspEI BspLU11I BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CpoI DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRV FauI FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AlwNI 1260 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 58 AGCGCT AGC^GCT AvaI 971 CCCGAG C^[CT]CG[GA]G BcgI 862 CGAGTTCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 348 CCCGG CC^[GC]GG BlpI 43 GCTGAGC GC^T[ACGT]AGC BpmI 1516 CTGGAG CTGGAG(16/14) Bpu1102I 43 GCTGAGC GC^T[ACGT]AGC BsoBI 971 CCCGAG C^[CT]CG[GA]G Bsp1407I 135 TGTACA T^GTACA BspDI 160 ATCGAT AT^CGAT BspMI 979 GCAGGT ACCTGC(4/8) BsrBI 1113 CCGCTC CCGCTC(-3/-3) BsrFI 504 ACCGGC [GA]^CCGG[CT] BsrGI 135 TGTACA T^GTACA CelII 43 GCTGAGC GC^T[ACGT]AGC Cfr10I 504 ACCGGC [GA]^CCGG[CT] ClaI 160 ATCGAT AT^CGAT DraI 1496 TTTAAA TTT^AAA DraII 1080 AGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 986 CCATGG C^C[GA][CT]GG EaeI 810 TGGCCG [CT]^GGCC[GA] Eco47III 58 AGCGCT AGC^GCT EcoO109I 1080 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 725 GAATTC G^AATTC EcoT22I 1150 ATGCAT ATGCA^T HaeII 58 AGCGCT [GA]GCGC^[CT] HincII 1420 GTCAAC GT[CT]^[GA]AC HindII 1420 GTCAAC GT[CT]^[GA]AC MspA1I 339 CAGCGG C[AC]G^C[GT]G NciI 348 CCCGG CC^[GC]GG NcoI 986 CCATGG C^CATGG NsiI 1150 ATGCAT ATGCA^T NspI 1221 GCATGC [GA]CATG^[CT] PflMI 1132 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1080 AGGTCCT [GA]G^G[AT]CC[CT] PstI 977 CTGCAG CTGCA^G SacI 545 GAGCTC GAGCT^C ScaI 449 AGTACT AGT^ACT SfaNI 1430 GATGC GCATC(5/9) SmlI 548 CTCAAG C^T[CT][GA]AG SphI 1221 GCATGC GCATG^C SspBI 135 TGTACA T^GTACA Van91I 1132 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1206 CCAGAAAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AspHI 517 GAGCAC G[AT]GC[AT]^C 545 GAGCTC AvaII 469 GGACC G^G[AT]CC 1081 GGTCC BamHI 775 GGATCC G^GATCC 921 GGATCC BanII 545 GAGCTC G[GA]GC[CT]^C 1047 GAGCCC BglII 835 AGATCT A^GATCT 1059 AGATCT BsaI 295 GGTCTC GGTCTC(1/5) 343 GGTCTC BseRI 38 GAGGAG GAGGAG(10/8) 542 GAGGAG BsiHKAI 517 GAGCAC G[AT]GC[AT]^C 545 GAGCTC BsiYI 931 CCGCCTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1132 CCACACAGTGG BslI 931 CCGCCTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1132 CCACACAGTGG BsmI 1148 GAATGC GAATGC(1/-1) 693 GCATTC BspHI 994 TCATGA T^CATGA 1478 TCATGA BsrDI 77 GCAATG GCAATG(2/0) 1415 GCAATG CfoI 59 GCGC GCG^C 415 GCGC DraIII 369 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 1133 CACACAGTG EcoT14I 986 CCATGG C^C[AT][AT]GG 1247 CCAAGG HaeIII 497 GGCC GG^CC 811 GGCC HapII 349 CCGG C^CGG 505 CCGG HhaI 59 GCGC GCG^C 415 GCGC HinP1I 59 GCGC G^CGC 415 GCGC HpaII 349 CCGG C^CGG 505 CCGG MaeIII 375 GTGAC ^GT[ACGT]AC 501 GTCAC MslI 326 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1094 CACCAATGTG MspI 349 CCGG C^CGG 505 CCGG MwoI 960 GCTTGGAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1548 GCTGCTCTTGC RcaI 994 TCATGA T^CATGA 1478 TCATGA SspI 641 AATATT AAT^ATT 822 AATATT StyI 986 CCATGG C^C[AT][AT]GG 1247 CCAAGG TaqI 161 TCGA T^CGA 861 TCGA Tsp45I 375 GTGAC ^GT[GC]AC 501 GTCAC TthHB8I 161 TCGA T^CGA 861 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I BbvI BfaI BmyI BsaJI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HinfI HphI ItaI MaeI MboI MboII MflI MnlI MseI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI TfiI Tru9I TseI Tsp509I TspRI XhoII XmnI