##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BanI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfrI BglI BlnI BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmFI
BspEI BspLU11I BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CpoI DrdI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRV FauI
FbaI FseI FspI HgaI Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AlwNI 1260 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 58 AGCGCT AGC^GCT
AvaI 971 CCCGAG C^[CT]CG[GA]G
BcgI 862 CGAGTTCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 348 CCCGG CC^[GC]GG
BlpI 43 GCTGAGC GC^T[ACGT]AGC
BpmI 1516 CTGGAG CTGGAG(16/14)
Bpu1102I 43 GCTGAGC GC^T[ACGT]AGC
BsoBI 971 CCCGAG C^[CT]CG[GA]G
Bsp1407I 135 TGTACA T^GTACA
BspDI 160 ATCGAT AT^CGAT
BspMI 979 GCAGGT ACCTGC(4/8)
BsrBI 1113 CCGCTC CCGCTC(-3/-3)
BsrFI 504 ACCGGC [GA]^CCGG[CT]
BsrGI 135 TGTACA T^GTACA
CelII 43 GCTGAGC GC^T[ACGT]AGC
Cfr10I 504 ACCGGC [GA]^CCGG[CT]
ClaI 160 ATCGAT AT^CGAT
DraI 1496 TTTAAA TTT^AAA
DraII 1080 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 986 CCATGG C^C[GA][CT]GG
EaeI 810 TGGCCG [CT]^GGCC[GA]
Eco47III 58 AGCGCT AGC^GCT
EcoO109I 1080 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 725 GAATTC G^AATTC
EcoT22I 1150 ATGCAT ATGCA^T
HaeII 58 AGCGCT [GA]GCGC^[CT]
HincII 1420 GTCAAC GT[CT]^[GA]AC
HindII 1420 GTCAAC GT[CT]^[GA]AC
MspA1I 339 CAGCGG C[AC]G^C[GT]G
NciI 348 CCCGG CC^[GC]GG
NcoI 986 CCATGG C^CATGG
NsiI 1150 ATGCAT ATGCA^T
NspI 1221 GCATGC [GA]CATG^[CT]
PflMI 1132 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1080 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 977 CTGCAG CTGCA^G
SacI 545 GAGCTC GAGCT^C
ScaI 449 AGTACT AGT^ACT
SfaNI 1430 GATGC GCATC(5/9)
SmlI 548 CTCAAG C^T[CT][GA]AG
SphI 1221 GCATGC GCATG^C
SspBI 135 TGTACA T^GTACA
Van91I 1132 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1206 CCAGAAAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AspHI 517 GAGCAC G[AT]GC[AT]^C
545 GAGCTC
AvaII 469 GGACC G^G[AT]CC
1081 GGTCC
BamHI 775 GGATCC G^GATCC
921 GGATCC
BanII 545 GAGCTC G[GA]GC[CT]^C
1047 GAGCCC
BglII 835 AGATCT A^GATCT
1059 AGATCT
BsaI 295 GGTCTC GGTCTC(1/5)
343 GGTCTC
BseRI 38 GAGGAG GAGGAG(10/8)
542 GAGGAG
BsiHKAI 517 GAGCAC G[AT]GC[AT]^C
545 GAGCTC
BsiYI 931 CCGCCTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1132 CCACACAGTGG
BslI 931 CCGCCTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1132 CCACACAGTGG
BsmI 1148 GAATGC GAATGC(1/-1)
693 GCATTC
BspHI 994 TCATGA T^CATGA
1478 TCATGA
BsrDI 77 GCAATG GCAATG(2/0)
1415 GCAATG
CfoI 59 GCGC GCG^C
415 GCGC
DraIII 369 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1133 CACACAGTG
EcoT14I 986 CCATGG C^C[AT][AT]GG
1247 CCAAGG
HaeIII 497 GGCC GG^CC
811 GGCC
HapII 349 CCGG C^CGG
505 CCGG
HhaI 59 GCGC GCG^C
415 GCGC
HinP1I 59 GCGC G^CGC
415 GCGC
HpaII 349 CCGG C^CGG
505 CCGG
MaeIII 375 GTGAC ^GT[ACGT]AC
501 GTCAC
MslI 326 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1094 CACCAATGTG
MspI 349 CCGG C^CGG
505 CCGG
MwoI 960 GCTTGGAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1548 GCTGCTCTTGC
RcaI 994 TCATGA T^CATGA
1478 TCATGA
SspI 641 AATATT AAT^ATT
822 AATATT
StyI 986 CCATGG C^C[AT][AT]GG
1247 CCAAGG
TaqI 161 TCGA T^CGA
861 TCGA
Tsp45I 375 GTGAC ^GT[GC]AC
501 GTCAC
TthHB8I 161 TCGA T^CGA
861 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I BbvI BfaI BmyI
BsaJI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HinfI HphI ItaI MaeI MboI
MboII MflI MnlI MseI MvaI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfcI TfiI Tru9I
TseI Tsp509I TspRI XhoII XmnI