##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AlwNI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BanI
BbeI BbrPI BcgI BciVI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstUI
BstXI CelII Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FauI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AciI 1052 GCGG CCGC(-3/-1)
AflIII 558 ACACGT A^C[GA][CT]GT
Alw44I 995 GTGCAC G^TGCAC
AocI 85 CCTCAGG CC^T[ACGT]AGG
ApaI 677 GGGCCC GGGCC^C
ApaLI 995 GTGCAC G^TGCAC
AspI 25 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 109 CCCGGG C^[CT]CG[GA]G
AvaII 169 GGACC G^G[AT]CC
BalI 415 TGGCCA TGG^CCA
BamHI 133 GGATCC G^GATCC
BbvCI 450 CCTCAGC CCTCAGC(-5/-2)
BclI 159 TGATCA T^GATCA
BfaI 863 CTAG C^TAG
BpmI 642 CTGGAG CTGGAG(16/14)
BsaI 1079 GAGACC GGTCTC(1/5)
BseRI 983 CTCCTC GAGGAG(10/8)
BsgI 709 CTGCAC GTGCAG(16/14)
BsmBI 599 CGTCTC CGTCTC(1/5)
BsmFI 742 GTCCC GGGAC(10/14)
BsoBI 109 CCCGGG C^[CT]CG[GA]G
BspHI 903 TCATGA T^CATGA
Bst1107I 1220 GTATAC GTA^TAC
BstEII 1281 GGTGACC G^GT[ACGT]ACC
BstPI 1281 GGTGACC G^GT[ACGT]ACC
BstYI 133 GGATCC [GA]^GATC[CT]
BstZ17I 1220 GTATAC GTA^TAC
Bsu36I 85 CCTCAGG CC^T[ACGT]AGG
CfoI 106 GCGC GCG^C
DraI 223 TTTAAA TTT^AAA
DraII 677 GGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 845 GAAGAG CTCTTC(1/4)
Eco81I 85 CCTCAGG CC^T[ACGT]AGG
EcoO109I 677 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1281 GGTGACC G^GT[ACGT]ACC
FbaI 159 TGATCA T^GATCA
HaeII 105 AGCGCC [GA]GCGC^[CT]
HhaI 106 GCGC GCG^C
HinP1I 106 GCGC G^CGC
Ksp632I 845 GAAGAG CTCTTC(1/4)
MaeI 863 CTAG C^TAG
MfeI 763 CAATTG C^AATTG
MflI 133 GGATCC [GA]^GATC[CT]
MluNI 415 TGGCCA TGG^CCA
MscI 415 TGGCCA TGG^CCA
MunI 763 CAATTG C^AATTG
NdeI 276 CATATG CA^TATG
PflFI 25 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 418 CCAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 740 GAGTC GAGTC(4/5)
PstI 101 CTGCAG CTGCA^G
RcaI 903 TCATGA T^CATGA
SacI 980 GAGCTC GAGCT^C
SmaI 109 CCCGGG CCC^GGG
SnaBI 484 TACGTA TAC^GTA
TaqI 544 TCGA T^CGA
Tsp45I 1282 GTGAC ^GT[GC]AC
Tth111I 25 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 544 TCGA T^CGA
Van91I 418 CCAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 133 GGATCC [GA]^GATC[CT]
XmaCI 109 CCCGGG C^CCGGG
XmaI 109 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 259 GTAC GT^AC
487 GTAC
AspHI 980 GAGCTC G[AT]GC[AT]^C
995 GTGCAC
BcnI 109 CCCGG CC^[GC]GG
110 CCGGG
BsaBI 728 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1019 GATAGCGATC
BsiHKAI 980 GAGCTC G[AT]GC[AT]^C
995 GTGCAC
BsmAI 1079 GAGAC GTCTC(1/5)
600 GTCTC
BstNI 440 CCAGG CC^[AT]GG
641 CCTGG
EaeI 415 TGGCCA [CT]^GGCC[GA]
616 TGGCCG
EcoRII 440 CCAGG ^CC[AT]GG
641 CCTGG
HapII 110 CCGG C^CGG
619 CCGG
HpaII 110 CCGG C^CGG
619 CCGG
MamI 728 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1019 GATAGCGATC
MslI 34 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
898 CATTGTCATG
MspA1I 392 CAGCTG C[AC]G^C[GT]G
873 CAGCTG
MspI 110 CCGG C^CGG
619 CCGG
MvaI 440 CCAGG CC^[AT]GG
641 CCTGG
MwoI 397 GCCCTTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
686 GCCATTCCAGC
NciI 109 CCCGG CC^[GC]GG
110 CCGGG
PvuII 392 CAGCTG CAG^CTG
873 CAGCTG
RsaI 259 GTAC GT^AC
487 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccI AcsI AluI AlwI ApoI
BanII BbsI BbvI BmyI BpuAI
BsaAI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I Cac8I Cfr13I
DdeI DpnII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp509I
TspRI