################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## Acc65I AccII AccIII AciI AclI AfaI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstUI BstXI Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRV FauI FseI FspI HaeII HgaI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI Sse8387I SspBI SspI SwaI Tth111I XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 1513 GACGTC GACGT^C AcyI 1513 GACGTC G[GA]^CG[CT]C AflIII 1294 ACACGT A^C[GA][CT]GT Alw44I 1276 GTGCAC G^TGCAC AlwNI 270 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1276 GTGCAC G^TGCAC AvaI 155 CTCGAG C^[CT]CG[GA]G BanII 594 GAGCCC G[GA]GC[CT]^C BclI 287 TGATCA T^GATCA BsaAI 408 TACGTA [CT]AC^GT[GA] BsaHI 1513 GACGTC G[GA]^CG[CT]C BsaWI 454 TCCGGT [AT]^CCGG[AT] BsmAI 1242 GAGAC GTCTC(1/5) BsmFI 458 GTCCC GGGAC(10/14) BsoBI 155 CTCGAG C^[CT]CG[GA]G BsrDI 1305 CATTGC GCAATG(2/0) Bst1107I 1828 GTATAC GTA^TAC BstEII 1045 GGTTACC G^GT[ACGT]ACC BstPI 1045 GGTTACC G^GT[ACGT]ACC BstZ17I 1828 GTATAC GTA^TAC CfoI 152 GCGC GCG^C DraIII 234 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 1045 GGTTACC G^GT[ACGT]ACC EcoRI 518 GAATTC G^AATTC EcoT14I 598 CCAAGG C^C[AT][AT]GG FbaI 287 TGATCA T^GATCA HapII 455 CCGG C^CGG HhaI 152 GCGC GCG^C Hin1I 1513 GACGTC G[GA]^CG[CT]C HinP1I 152 GCGC G^CGC HpaII 455 CCGG C^CGG MspI 455 CCGG C^CGG PaeR7I 155 CTCGAG C^TCGAG PflMI 269 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 883 GACGGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 422 CTGCAG CTGCA^G SapI 762 GAAGAGC GCTCTTC(1/4) SmlI 155 CTCGAG C^T[CT][GA]AG SnaBI 408 TACGTA TAC^GTA SpeI 873 ACTAGT A^CTAGT SphI 131 GCATGC GCATG^C StuI 954 AGGCCT AGG^CCT StyI 598 CCAAGG C^C[AT][AT]GG Tsp45I 272 GTCAC ^GT[GC]AC Van91I 269 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 155 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 1440 GTAGAC GT^[AC][GT]AC 1828 GTATAC AspHI 754 GAGCAC G[AT]GC[AT]^C 1276 GTGCAC AvaII 457 GGTCC G^G[AT]CC 735 GGACC BbvI 219 GCTGC GCAGC(8/12) 222 GCTGC BsaJI 598 CCAAGG C^C[ACGT][ACGT]GG 731 CCCAGG BsiHKAI 754 GAGCAC G[AT]GC[AT]^C 1276 GTGCAC BstYI 1203 AGATCC [GA]^GATC[CT] 1262 AGATCC Cac8I 131 GCATGC GC[ACGT]^[ACGT]GC 1388 GCCTGC EcoT22I 87 ATGCAT ATGCA^T 129 ATGCAT Fnu4HI 219 GCTGC GC^[ACGT]GC 222 GCTGC HphI 312 TCACC GGTGA(8/7) 430 TCACC ItaI 219 GCTGC GC^[ACGT]GC 222 GCTGC MflI 1203 AGATCC [GA]^GATC[CT] 1262 AGATCC MseI 70 TTAA T^TAA 487 TTAA MslI 301 CATCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 566 CATCAAAATG MwoI 415 GCCATATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1625 GCTCATCAAGC NdeI 447 CATATG CA^TATG 802 CATATG NlaIV 457 GGTCCC GG[ACGT]^[ACGT]CC 983 GGCTCC NsiI 87 ATGCAT ATGCA^T 129 ATGCAT TaqI 156 TCGA T^CGA 1688 TCGA Tru9I 70 TTAA T^TAA 487 TTAA TseI 219 GCTGC G^C[AT]GC 222 GCTGC TthHB8I 156 TCGA T^CGA 1688 TCGA XhoII 1203 AGATCC [GA]^GATC[CT] 1262 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AcsI AluI AlwI ApoI BfaI BmyI BpmI BseRI BsgI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRII FokI HaeIII HincII HindII HinfI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MvaI NdeII NlaIII NspI PleI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tsp509I TspRI XbaI