##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

Acc65I      AccII       AccIII      AciI        AclI        
AfaI        AflII       AgeI        AhdI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaI        
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       BstAPI      BstBI       BstUI       BstXI       
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraII       DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoRV       FauI        
FseI        FspI        HaeII       HgaI        HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspA1I      MunI        MvnI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XcmI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII        1513  GACGTC               GACGT^C
AcyI         1513  GACGTC               G[GA]^CG[CT]C
AflIII       1294  ACACGT               A^C[GA][CT]GT
Alw44I       1276  GTGCAC               G^TGCAC
AlwNI         270  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1276  GTGCAC               G^TGCAC
AvaI          155  CTCGAG               C^[CT]CG[GA]G
BanII         594  GAGCCC               G[GA]GC[CT]^C
BclI          287  TGATCA               T^GATCA
BsaAI         408  TACGTA               [CT]AC^GT[GA]
BsaHI        1513  GACGTC               G[GA]^CG[CT]C
BsaWI         454  TCCGGT               [AT]^CCGG[AT]
BsmAI        1242  GAGAC                GTCTC(1/5)
BsmFI         458  GTCCC                GGGAC(10/14)
BsoBI         155  CTCGAG               C^[CT]CG[GA]G
BsrDI        1305  CATTGC               GCAATG(2/0)
Bst1107I     1828  GTATAC               GTA^TAC
BstEII       1045  GGTTACC              G^GT[ACGT]ACC
BstPI        1045  GGTTACC              G^GT[ACGT]ACC
BstZ17I      1828  GTATAC               GTA^TAC
CfoI          152  GCGC                 GCG^C
DraIII        234  CACTATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      1045  GGTTACC              G^GT[ACGT]ACC
EcoRI         518  GAATTC               G^AATTC
EcoT14I       598  CCAAGG               C^C[AT][AT]GG
FbaI          287  TGATCA               T^GATCA
HapII         455  CCGG                 C^CGG
HhaI          152  GCGC                 GCG^C
Hin1I        1513  GACGTC               G[GA]^CG[CT]C
HinP1I        152  GCGC                 G^CGC
HpaII         455  CCGG                 C^CGG
MspI          455  CCGG                 C^CGG
PaeR7I        155  CTCGAG               C^TCGAG
PflMI         269  CCAGTCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         883  GACGGATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          422  CTGCAG               CTGCA^G
SapI          762  GAAGAGC              GCTCTTC(1/4)
SmlI          155  CTCGAG               C^T[CT][GA]AG
SnaBI         408  TACGTA               TAC^GTA
SpeI          873  ACTAGT               A^CTAGT
SphI          131  GCATGC               GCATG^C
StuI          954  AGGCCT               AGG^CCT
StyI          598  CCAAGG               C^C[AT][AT]GG
Tsp45I        272  GTCAC                ^GT[GC]AC
Van91I        269  CCAGTCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          155  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI         1440  GTAGAC               GT^[AC][GT]AC
             1828  GTATAC              
AspHI         754  GAGCAC               G[AT]GC[AT]^C
             1276  GTGCAC              
AvaII         457  GGTCC                G^G[AT]CC
              735  GGACC               
BbvI          219  GCTGC                GCAGC(8/12)
              222  GCTGC               
BsaJI         598  CCAAGG               C^C[ACGT][ACGT]GG
              731  CCCAGG              
BsiHKAI       754  GAGCAC               G[AT]GC[AT]^C
             1276  GTGCAC              
BstYI        1203  AGATCC               [GA]^GATC[CT]
             1262  AGATCC              
Cac8I         131  GCATGC               GC[ACGT]^[ACGT]GC
             1388  GCCTGC              
EcoT22I        87  ATGCAT               ATGCA^T
              129  ATGCAT              
Fnu4HI        219  GCTGC                GC^[ACGT]GC
              222  GCTGC               
HphI          312  TCACC                GGTGA(8/7)
              430  TCACC               
ItaI          219  GCTGC                GC^[ACGT]GC
              222  GCTGC               
MflI         1203  AGATCC               [GA]^GATC[CT]
             1262  AGATCC              
MseI           70  TTAA                 T^TAA
              487  TTAA                
MslI          301  CATCCACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              566  CATCAAAATG          
MwoI          415  GCCATATCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1625  GCTCATCAAGC         
NdeI          447  CATATG               CA^TATG
              802  CATATG              
NlaIV         457  GGTCCC               GG[ACGT]^[ACGT]CC
              983  GGCTCC              
NsiI           87  ATGCAT               ATGCA^T
              129  ATGCAT              
TaqI          156  TCGA                 T^CGA
             1688  TCGA                
Tru9I          70  TTAA                 T^TAA
              487  TTAA                
TseI          219  GCTGC                G^C[AT]GC
              222  GCTGC               
TthHB8I       156  TCGA                 T^CGA
             1688  TCGA                
XhoII        1203  AGATCC               [GA]^GATC[CT]
             1262  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        BfaI        
BmyI        BpmI        BseRI       BsgI        BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       EarI        
EcoRII      FokI        HaeIII      HincII      HindII      
HinfI       Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MvaI        NdeII       
NlaIII      NspI        PleI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TfiI        Tsp509I     
TspRI       XbaI