##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
Acc65I AccII AccIII AciI AclI
AfaI AflII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstUI BstXI
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRV FauI
FseI FspI HaeII HgaI HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI Sse8387I SspBI SspI
SwaI Tth111I XcmI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1513 GACGTC GACGT^C
AcyI 1513 GACGTC G[GA]^CG[CT]C
AflIII 1294 ACACGT A^C[GA][CT]GT
Alw44I 1276 GTGCAC G^TGCAC
AlwNI 270 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1276 GTGCAC G^TGCAC
AvaI 155 CTCGAG C^[CT]CG[GA]G
BanII 594 GAGCCC G[GA]GC[CT]^C
BclI 287 TGATCA T^GATCA
BsaAI 408 TACGTA [CT]AC^GT[GA]
BsaHI 1513 GACGTC G[GA]^CG[CT]C
BsaWI 454 TCCGGT [AT]^CCGG[AT]
BsmAI 1242 GAGAC GTCTC(1/5)
BsmFI 458 GTCCC GGGAC(10/14)
BsoBI 155 CTCGAG C^[CT]CG[GA]G
BsrDI 1305 CATTGC GCAATG(2/0)
Bst1107I 1828 GTATAC GTA^TAC
BstEII 1045 GGTTACC G^GT[ACGT]ACC
BstPI 1045 GGTTACC G^GT[ACGT]ACC
BstZ17I 1828 GTATAC GTA^TAC
CfoI 152 GCGC GCG^C
DraIII 234 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1045 GGTTACC G^GT[ACGT]ACC
EcoRI 518 GAATTC G^AATTC
EcoT14I 598 CCAAGG C^C[AT][AT]GG
FbaI 287 TGATCA T^GATCA
HapII 455 CCGG C^CGG
HhaI 152 GCGC GCG^C
Hin1I 1513 GACGTC G[GA]^CG[CT]C
HinP1I 152 GCGC G^CGC
HpaII 455 CCGG C^CGG
MspI 455 CCGG C^CGG
PaeR7I 155 CTCGAG C^TCGAG
PflMI 269 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 883 GACGGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 422 CTGCAG CTGCA^G
SapI 762 GAAGAGC GCTCTTC(1/4)
SmlI 155 CTCGAG C^T[CT][GA]AG
SnaBI 408 TACGTA TAC^GTA
SpeI 873 ACTAGT A^CTAGT
SphI 131 GCATGC GCATG^C
StuI 954 AGGCCT AGG^CCT
StyI 598 CCAAGG C^C[AT][AT]GG
Tsp45I 272 GTCAC ^GT[GC]AC
Van91I 269 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 155 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1440 GTAGAC GT^[AC][GT]AC
1828 GTATAC
AspHI 754 GAGCAC G[AT]GC[AT]^C
1276 GTGCAC
AvaII 457 GGTCC G^G[AT]CC
735 GGACC
BbvI 219 GCTGC GCAGC(8/12)
222 GCTGC
BsaJI 598 CCAAGG C^C[ACGT][ACGT]GG
731 CCCAGG
BsiHKAI 754 GAGCAC G[AT]GC[AT]^C
1276 GTGCAC
BstYI 1203 AGATCC [GA]^GATC[CT]
1262 AGATCC
Cac8I 131 GCATGC GC[ACGT]^[ACGT]GC
1388 GCCTGC
EcoT22I 87 ATGCAT ATGCA^T
129 ATGCAT
Fnu4HI 219 GCTGC GC^[ACGT]GC
222 GCTGC
HphI 312 TCACC GGTGA(8/7)
430 TCACC
ItaI 219 GCTGC GC^[ACGT]GC
222 GCTGC
MflI 1203 AGATCC [GA]^GATC[CT]
1262 AGATCC
MseI 70 TTAA T^TAA
487 TTAA
MslI 301 CATCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
566 CATCAAAATG
MwoI 415 GCCATATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1625 GCTCATCAAGC
NdeI 447 CATATG CA^TATG
802 CATATG
NlaIV 457 GGTCCC GG[ACGT]^[ACGT]CC
983 GGCTCC
NsiI 87 ATGCAT ATGCA^T
129 ATGCAT
TaqI 156 TCGA T^CGA
1688 TCGA
Tru9I 70 TTAA T^TAA
487 TTAA
TseI 219 GCTGC G^C[AT]GC
222 GCTGC
TthHB8I 156 TCGA T^CGA
1688 TCGA
XhoII 1203 AGATCC [GA]^GATC[CT]
1262 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AluI AlwI ApoI BfaI
BmyI BpmI BseRI BsgI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EarI
EcoRII FokI HaeIII HincII HindII
HinfI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MvaI NdeII
NlaIII NspI PleI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tsp509I
TspRI XbaI