##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BbrPI BciVI
BclI BfrI BglI BlnI BsaAI
BsaBI BsaI BseAI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraII DraIII
EagI Eam1105I EarI EclXI Eco52I
EcoO109I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HgaI HpaI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI
NspV PacI PflFI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccI 927 GTCGAC GT^[AC][GT]AC
AcyI 29 GGCGCC G[GA]^CG[CT]C
AgeI 1309 ACCGGT A^CCGGT
AlwNI 322 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 230 CCTCAGG CC^T[ACGT]AGG
Aor51HI 853 AGCGCT AGC^GCT
Asp700I 1226 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1156 CTCGAG C^[CT]CG[GA]G
BalI 220 TGGCCA TGG^CCA
BamHI 948 GGATCC G^GATCC
BanI 29 GGCGCC G^G[CT][GA]CC
BanII 1258 GGGCTC G[GA]GC[CT]^C
BbeI 29 GGCGCC GGCGC^C
BbsI 980 GAAGAC GAAGAC(2/6)
BbvCI 837 CCTCAGC CCTCAGC(-5/-2)
BcgI 83 GCACGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1202 CCCGG CC^[GC]GG
BfaI 681 CTAG C^TAG
BlpI 351 GCTGAGC GC^T[ACGT]AGC
BmyI 1258 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 351 GCTGAGC GC^T[ACGT]AGC
BpuAI 980 GAAGAC GAAGAC(2/6)
BsaHI 29 GGCGCC G[GA]^CG[CT]C
BsaWI 1309 ACCGGT [AT]^CCGG[AT]
BseRI 39 GAGGAG GAGGAG(10/8)
BsmFI 251 GGGAC GGGAC(10/14)
BsmI 1034 GAATGC GAATGC(1/-1)
BsoBI 1156 CTCGAG C^[CT]CG[GA]G
Bsp1286I 1258 GGGCTC G[AGT]GC[ACT]^C
BspHI 1009 TCATGA T^CATGA
BsrFI 1309 ACCGGT [GA]^CCGG[CT]
BssSI 84 CACGAG CACGAG(-5/-1)
Bsu36I 230 CCTCAGG CC^T[ACGT]AGG
CelII 351 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1309 ACCGGT [GA]^CCGG[CT]
DraI 578 TTTAAA TTT^AAA
DrdI 639 GACGTGTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 853 AGCGCT AGC^GCT
Eco81I 230 CCTCAGG CC^T[ACGT]AGG
EcoNI 5 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1526 CCAAGG C^C[AT][AT]GG
Hin1I 29 GGCGCC G[GA]^CG[CT]C
HincII 927 GTCGAC GT[CT]^[GA]AC
HindII 927 GTCGAC GT[CT]^[GA]AC
HindIII 1330 AAGCTT A^AGCTT
KasI 29 GGCGCC G^GCGCC
MaeI 681 CTAG C^TAG
MluNI 220 TGGCCA TGG^CCA
MscI 220 TGGCCA TGG^CCA
MslI 261 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 29 GGCGCC GG^CGCC
NciI 1202 CCCGG CC^[GC]GG
NruI 92 TCGCGA TCG^CGA
PaeR7I 1156 CTCGAG C^TCGAG
PflMI 197 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1309 ACCGGT A^CCGGT
RcaI 1009 TCATGA T^CATGA
SalI 927 GTCGAC G^TCGAC
SfoI 29 GGCGCC GGC^GCC
StyI 1526 CCAAGG C^C[AT][AT]GG
Van91I 197 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1156 CTCGAG C^TCGAG
XmnI 1226 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 19 CGCG CG^CG
93 CGCG
AfaI 589 GTAC GT^AC
1430 GTAC
AflIII 640 ACGTGT A^C[GA][CT]GT
1001 ACGTGT
AvaII 1307 GGACC G^G[AT]CC
1443 GGTCC
BglII 844 AGATCT A^GATCT
1393 AGATCT
BsgI 81 CTGCAC GTGCAG(16/14)
66 GTGCAG
BspMI 241 ACCTGC ACCTGC(4/8)
478 ACCTGC
BstAPI 68 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1029 GCAAAGAATGC
BstUI 19 CGCG CG^CG
93 CGCG
Cfr13I 1307 GGACC G^G[ACGT]CC
1443 GGTCC
DsaI 1042 CCACGG C^C[GA][CT]GG
1178 CCGTGG
EaeI 220 TGGCCA [CT]^GGCC[GA]
820 TGGCCG
EcoRV 737 GATATC GAT^ATC
1021 GATATC
HaeII 29 GGCGCC [GA]GCGC^[CT]
853 AGCGCT
HapII 1203 CCGG C^CGG
1310 CCGG
HpaII 1203 CCGG C^CGG
1310 CCGG
MspI 1203 CCGG C^CGG
1310 CCGG
MvnI 19 CGCG CG^CG
93 CGCG
PstI 54 CTGCAG CTGCA^G
243 CTGCAG
RsaI 589 GTAC GT^AC
1430 GTAC
Sau96I 1307 GGACC G^G[ACGT]CC
1443 GGTCC
SfaNI 1068 GATGC GCATC(5/9)
1341 GATGC
SfcI 54 CTGCAG C^T[GA][CT]AG
243 CTGCAG
SmlI 715 CTCAAG C^T[CT][GA]AG
1156 CTCGAG
Tsp45I 606 GTGAC ^GT[GC]AC
672 GTGAC
XcmI 592 CCACAAGTGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1345 CCAAATATGAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BpmI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
ScrFI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII