##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
AccI AccII AccIII AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AspEI AspI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbsI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT22I FauI FseI
FspI HaeII HgaI HhaI HinP1I
HindIII HpaI KasI KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 850 GACGTC GACGT^C
Acc65I 1833 GGTACC G^GTACC
AclI 133 AACGTT AA^CGTT
AcyI 850 GACGTC G[GA]^CG[CT]C
AflIII 67 ACACGT A^C[GA][CT]GT
Asp718I 1833 GGTACC G^GTACC
BbvCI 1815 CCTCAGC CCTCAGC(-5/-2)
BcnI 822 CCGGG CC^[GC]GG
BfaI 1368 CTAG C^TAG
BsaAI 1189 CACGTA [CT]AC^GT[GA]
BsaBI 1039 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 850 GACGTC G[GA]^CG[CT]C
BssSI 751 CACGAG CACGAG(-5/-1)
BstAPI 1158 GCATCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1044 GGATCT [GA]^GATC[CT]
DraIII 804 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 953 GAATTC G^AATTC
HapII 822 CCGG C^CGG
Hin1I 850 GACGTC G[GA]^CG[CT]C
HincII 1508 GTTGAC GT[CT]^[GA]AC
HindII 1508 GTTGAC GT[CT]^[GA]AC
HpaII 822 CCGG C^CGG
KpnI 1833 GGTACC GGTAC^C
MaeI 1368 CTAG C^TAG
MamI 1039 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1044 GGATCT [GA]^GATC[CT]
MspA1I 335 CAGCTG C[AC]G^C[GT]G
MspI 822 CCGG C^CGG
NciI 822 CCGGG CC^[GC]GG
NcoI 729 CCATGG C^CATGG
PpuMI 1254 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 757 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 133 AACGTT AA^CGTT
PstI 2015 CTGCAG CTGCA^G
PvuII 335 CAGCTG CAG^CTG
SapI 1448 GCTCTTC GCTCTTC(1/4)
SfcI 2015 CTGCAG C^T[GA][CT]AG
Tsp45I 1490 GTGAC ^GT[GC]AC
XhoII 1044 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 27 GGATC GGATC(4/5)
1044 GGATC
AlwNI 332 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1583 CAGGGCCTG
AseI 1456 ATTAAT AT^TAAT
1921 ATTAAT
AsnI 1456 ATTAAT AT^TAAT
1921 ATTAAT
AspHI 576 GAGCAC G[AT]GC[AT]^C
1087 GAGCAC
BanI 544 GGTGCC G^G[CT][GA]CC
1833 GGTACC
BclI 555 TGATCA T^GATCA
1977 TGATCA
BmyI 576 GAGCAC G[AGT]GC[ACT]^C
1087 GAGCAC
BpmI 145 CTCCAG CTGGAG(16/14)
573 CTGGAG
BseRI 881 GAGGAG GAGGAG(10/8)
948 GAGGAG
BsiHKAI 576 GAGCAC G[AT]GC[AT]^C
1087 GAGCAC
BsmAI 511 GAGAC GTCTC(1/5)
1364 GAGAC
BsmI 472 GCATTC GAATGC(1/-1)
1963 GCATTC
Bsp1286I 576 GAGCAC G[AGT]GC[ACT]^C
1087 GAGCAC
BspHI 72 TCATGA T^CATGA
918 TCATGA
BspMI 617 GCAGGT ACCTGC(4/8)
2017 GCAGGT
DraII 1254 AGGTCCC [GA]G^G[ACGT]CC[CT]
1584 AGGGCCT
DsaI 729 CCATGG C^C[GA][CT]GG
1779 CCGTGG
EcoO109I 1254 AGGTCCC [GA]G^G[ACGT]CC[CT]
1584 AGGGCCT
EcoRV 377 GATATC GAT^ATC
2037 GATATC
FbaI 555 TGATCA T^GATCA
1977 TGATCA
MaeIII 131 GTAAC ^GT[ACGT]AC
1490 GTGAC
NspI 827 GCATGC [GA]CATG^[CT]
1842 GCATGC
PshBI 1456 ATTAAT AT^TAAT
1921 ATTAAT
RcaI 72 TCATGA T^CATGA
918 TCATGA
SphI 827 GCATGC GCATG^C
1842 GCATGC
SspI 717 AATATT AAT^ATT
1659 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AvaII BbvI BsaJI BsiYI
BslI BsmFI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
Tru9I TseI Tsp509I TspRI XcmI
XmnI