##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AclI        AflII       AflIII      AgeI        Alw44I      
AlwI        AocI        ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     AspI        AvaI        
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BlnI        
BsaAI       BsaBI       BsiEI       BsiWI       BsmBI       
BsoBI       Bsp1407I    BspHI       BspLU11I    BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CpoI        
DraI        DrdI        EagI        EclXI       Eco52I      
Eco81I      EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        HgaI        HpaI        MamI        MfeI        
MluI        MseI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       Tth111I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AatII         448  GACGTC               GACGT^C
Acc65I        497  GGTACC               G^GTACC
AccI          713  GTCTAC               GT^[AC][GT]AC
AccII        1541  CGCG                 CG^CG
AccIII       1617  TCCGGA               T^CCGGA
AcsI         1611  GAATTC               [GA]^AATT[CT]
AlwNI        1007  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       131  AGCGCT               AGC^GCT
ApoI         1611  GAATTC               [GA]^AATT[CT]
Asp718I       497  GGTACC               G^GTACC
AspHI         857  GAGCTC               G[AT]GC[AT]^C
BlpI         1471  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     1471  GCTTAGC              GC^T[ACGT]AGC
BsaWI        1617  TCCGGA               [AT]^CCGG[AT]
BseAI        1617  TCCGGA               T^CCGGA
BsgI         1307  CTGCAC               GTGCAG(16/14)
BsiHKAI       857  GAGCTC               G[AT]GC[AT]^C
BsmI          437  GCATTC               GAATGC(1/-1)
BspDI         863  ATCGAT               AT^CGAT
BspEI        1617  TCCGGA               T^CCGGA
BspMI         123  ACCTGC               ACCTGC(4/8)
BsrBI        1272  GAGCGG               CCGCTC(-3/-3)
BsrFI         141  GCCGGC               [GA]^CCGG[CT]
BstUI        1541  CGCG                 CG^CG
CelII        1471  GCTTAGC              GC^T[ACGT]AGC
Cfr10I        141  GCCGGC               [GA]^CCGG[CT]
ClaI          863  ATCGAT               AT^CGAT
DraIII        319  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         1217  CTCTTC               CTCTTC(1/4)
Eco47III      131  AGCGCT               AGC^GCT
EcoNI         337  CCTCCCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1611  GAATTC               G^AATTC
HincII        845  GTCAAC               GT[CT]^[GA]AC
HindII        845  GTCAAC               GT[CT]^[GA]AC
HindIII      1469  AAGCTT               A^AGCTT
KpnI          497  GGTACC               GGTAC^C
Ksp632I      1217  CTCTTC               CTCTTC(1/4)
KspI         1540  CCGCGG               CCGC^GG
MroI         1617  TCCGGA               T^CCGGA
MslI          229  CACCTTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI         1541  CGCG                 CG^CG
NaeI          141  GCCGGC               GCC^GGC
NcoI           72  CCATGG               C^CATGG
NgoMIV        141  GCCGGC               G^CCGGC
NspI          362  ACATGC               [GA]CATG^[CT]
PpuMI         168  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII        1511  CAGCTG               CAG^CTG
SacI          857  GAGCTC               GAGCT^C
SacII        1540  CCGCGG               CCGC^GG
SmlI          351  CTCAAG               C^T[CT][GA]AG
StuI          952  AGGCCT               AGG^CCT
TaqI          864  TCGA                 T^CGA
TthHB8I       864  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AfaI           83  GTAC                 GT^AC
              498  GTAC                
AhdI          619  GACAGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1199  GACCAGAAGTC         
AspEI         619  GACAGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1199  GACCAGAAGTC         
AviII          36  TGCGCA               TGC^GCA
             1014  TGCGCA              
BalI          390  TGGCCA               TGG^CCA
              567  TGGCCA              
BbeI          144  GGCGCC               GGCGC^C
              907  GGCGCC              
BbsI           47  GTCTTC               GAAGAC(2/6)
             1211  GTCTTC              
BcnI          511  CCCGG                CC^[GC]GG
             1283  CCCGG               
BfaI         1566  CTAG                 C^TAG
             1600  CTAG                
BpuAI          47  GTCTTC               GAAGAC(2/6)
             1211  GTCTTC              
BsaI          120  GAGACC               GGTCTC(1/5)
             1520  GAGACC              
BseRI         344  GAGGAG               GAGGAG(10/8)
              815  GAGGAG              
BstAPI        437  GCATTCAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1232  GCACTGACTGC         
BstXI          72  CCATGGTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1326  CCATCCTCCTGG        
DraII         168  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1008  AGGGCCT             
DsaI           72  CCATGG               C^C[GA][CT]GG
             1540  CCGCGG              
Eam1105I      619  GACAGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1199  GACCAGAAGTC         
EcoO109I      168  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1008  AGGGCCT             
FspI           36  TGCGCA               TGC^GCA
             1014  TGCGCA              
KasI          144  GGCGCC               G^GCGCC
              907  GGCGCC              
MaeI         1566  CTAG                 C^TAG
             1600  CTAG                
MluNI         390  TGGCCA               TGG^CCA
              567  TGGCCA              
MscI          390  TGGCCA               TGG^CCA
              567  TGGCCA              
MspA1I       1511  CAGCTG               C[AC]G^C[GT]G
             1540  CCGCGG              
NarI          144  GGCGCC               GG^CGCC
              907  GGCGCC              
NciI          511  CCCGG                CC^[GC]GG
             1283  CCCGG               
PflMI        1051  CCAGACATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1131  CCATCGTCTGG         
RsaI           83  GTAC                 GT^AC
              498  GTAC                
SfoI          144  GGCGCC               GGC^GCC
              907  GGCGCC              
Van91I       1051  CCAGACATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1131  CCATCGTCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AcyI        AluI        AvaII       BanI        
BanII       BbvI        BglII       BmyI        BpmI        
BsaHI       BsaJI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EaeI        EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        PstI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       XcmI        XhoII