##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AclI AflII AflIII AgeI Alw44I
AlwI AocI ApaI ApaLI AscI
AseI AsnI Asp700I AspI AvaI
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BsaAI BsaBI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CpoI
DraI DrdI EagI EclXI Eco52I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI HgaI HpaI MamI MfeI
MluI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 448 GACGTC GACGT^C
Acc65I 497 GGTACC G^GTACC
AccI 713 GTCTAC GT^[AC][GT]AC
AccII 1541 CGCG CG^CG
AccIII 1617 TCCGGA T^CCGGA
AcsI 1611 GAATTC [GA]^AATT[CT]
AlwNI 1007 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 131 AGCGCT AGC^GCT
ApoI 1611 GAATTC [GA]^AATT[CT]
Asp718I 497 GGTACC G^GTACC
AspHI 857 GAGCTC G[AT]GC[AT]^C
BlpI 1471 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1471 GCTTAGC GC^T[ACGT]AGC
BsaWI 1617 TCCGGA [AT]^CCGG[AT]
BseAI 1617 TCCGGA T^CCGGA
BsgI 1307 CTGCAC GTGCAG(16/14)
BsiHKAI 857 GAGCTC G[AT]GC[AT]^C
BsmI 437 GCATTC GAATGC(1/-1)
BspDI 863 ATCGAT AT^CGAT
BspEI 1617 TCCGGA T^CCGGA
BspMI 123 ACCTGC ACCTGC(4/8)
BsrBI 1272 GAGCGG CCGCTC(-3/-3)
BsrFI 141 GCCGGC [GA]^CCGG[CT]
BstUI 1541 CGCG CG^CG
CelII 1471 GCTTAGC GC^T[ACGT]AGC
Cfr10I 141 GCCGGC [GA]^CCGG[CT]
ClaI 863 ATCGAT AT^CGAT
DraIII 319 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1217 CTCTTC CTCTTC(1/4)
Eco47III 131 AGCGCT AGC^GCT
EcoNI 337 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1611 GAATTC G^AATTC
HincII 845 GTCAAC GT[CT]^[GA]AC
HindII 845 GTCAAC GT[CT]^[GA]AC
HindIII 1469 AAGCTT A^AGCTT
KpnI 497 GGTACC GGTAC^C
Ksp632I 1217 CTCTTC CTCTTC(1/4)
KspI 1540 CCGCGG CCGC^GG
MroI 1617 TCCGGA T^CCGGA
MslI 229 CACCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1541 CGCG CG^CG
NaeI 141 GCCGGC GCC^GGC
NcoI 72 CCATGG C^CATGG
NgoMIV 141 GCCGGC G^CCGGC
NspI 362 ACATGC [GA]CATG^[CT]
PpuMI 168 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 1511 CAGCTG CAG^CTG
SacI 857 GAGCTC GAGCT^C
SacII 1540 CCGCGG CCGC^GG
SmlI 351 CTCAAG C^T[CT][GA]AG
StuI 952 AGGCCT AGG^CCT
TaqI 864 TCGA T^CGA
TthHB8I 864 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 83 GTAC GT^AC
498 GTAC
AhdI 619 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1199 GACCAGAAGTC
AspEI 619 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1199 GACCAGAAGTC
AviII 36 TGCGCA TGC^GCA
1014 TGCGCA
BalI 390 TGGCCA TGG^CCA
567 TGGCCA
BbeI 144 GGCGCC GGCGC^C
907 GGCGCC
BbsI 47 GTCTTC GAAGAC(2/6)
1211 GTCTTC
BcnI 511 CCCGG CC^[GC]GG
1283 CCCGG
BfaI 1566 CTAG C^TAG
1600 CTAG
BpuAI 47 GTCTTC GAAGAC(2/6)
1211 GTCTTC
BsaI 120 GAGACC GGTCTC(1/5)
1520 GAGACC
BseRI 344 GAGGAG GAGGAG(10/8)
815 GAGGAG
BstAPI 437 GCATTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1232 GCACTGACTGC
BstXI 72 CCATGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1326 CCATCCTCCTGG
DraII 168 GGGACCT [GA]G^G[ACGT]CC[CT]
1008 AGGGCCT
DsaI 72 CCATGG C^C[GA][CT]GG
1540 CCGCGG
Eam1105I 619 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1199 GACCAGAAGTC
EcoO109I 168 GGGACCT [GA]G^G[ACGT]CC[CT]
1008 AGGGCCT
FspI 36 TGCGCA TGC^GCA
1014 TGCGCA
KasI 144 GGCGCC G^GCGCC
907 GGCGCC
MaeI 1566 CTAG C^TAG
1600 CTAG
MluNI 390 TGGCCA TGG^CCA
567 TGGCCA
MscI 390 TGGCCA TGG^CCA
567 TGGCCA
MspA1I 1511 CAGCTG C[AC]G^C[GT]G
1540 CCGCGG
NarI 144 GGCGCC GG^CGCC
907 GGCGCC
NciI 511 CCCGG CC^[GC]GG
1283 CCCGG
PflMI 1051 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1131 CCATCGTCTGG
RsaI 83 GTAC GT^AC
498 GTAC
SfoI 144 GGCGCC GGC^GCC
907 GGCGCC
Van91I 1051 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1131 CCATCGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcyI AluI AvaII BanI
BanII BbvI BglII BmyI BpmI
BsaHI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI TseI
Tsp45I Tsp509I TspRI XcmI XhoII