################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AclI AflII AflIII AgeI Alw44I AlwI AocI ApaI ApaLI AscI AseI AsnI Asp700I AspI AvaI AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BsaAI BsaBI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CpoI DraI DrdI EagI EclXI Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI HgaI HpaI MamI MfeI MluI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 448 GACGTC GACGT^C Acc65I 497 GGTACC G^GTACC AccI 713 GTCTAC GT^[AC][GT]AC AccII 1541 CGCG CG^CG AccIII 1617 TCCGGA T^CCGGA AcsI 1611 GAATTC [GA]^AATT[CT] AlwNI 1007 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 131 AGCGCT AGC^GCT ApoI 1611 GAATTC [GA]^AATT[CT] Asp718I 497 GGTACC G^GTACC AspHI 857 GAGCTC G[AT]GC[AT]^C BlpI 1471 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1471 GCTTAGC GC^T[ACGT]AGC BsaWI 1617 TCCGGA [AT]^CCGG[AT] BseAI 1617 TCCGGA T^CCGGA BsgI 1307 CTGCAC GTGCAG(16/14) BsiHKAI 857 GAGCTC G[AT]GC[AT]^C BsmI 437 GCATTC GAATGC(1/-1) BspDI 863 ATCGAT AT^CGAT BspEI 1617 TCCGGA T^CCGGA BspMI 123 ACCTGC ACCTGC(4/8) BsrBI 1272 GAGCGG CCGCTC(-3/-3) BsrFI 141 GCCGGC [GA]^CCGG[CT] BstUI 1541 CGCG CG^CG CelII 1471 GCTTAGC GC^T[ACGT]AGC Cfr10I 141 GCCGGC [GA]^CCGG[CT] ClaI 863 ATCGAT AT^CGAT DraIII 319 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 1217 CTCTTC CTCTTC(1/4) Eco47III 131 AGCGCT AGC^GCT EcoNI 337 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1611 GAATTC G^AATTC HincII 845 GTCAAC GT[CT]^[GA]AC HindII 845 GTCAAC GT[CT]^[GA]AC HindIII 1469 AAGCTT A^AGCTT KpnI 497 GGTACC GGTAC^C Ksp632I 1217 CTCTTC CTCTTC(1/4) KspI 1540 CCGCGG CCGC^GG MroI 1617 TCCGGA T^CCGGA MslI 229 CACCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 1541 CGCG CG^CG NaeI 141 GCCGGC GCC^GGC NcoI 72 CCATGG C^CATGG NgoMIV 141 GCCGGC G^CCGGC NspI 362 ACATGC [GA]CATG^[CT] PpuMI 168 GGGACCT [GA]G^G[AT]CC[CT] PvuII 1511 CAGCTG CAG^CTG SacI 857 GAGCTC GAGCT^C SacII 1540 CCGCGG CCGC^GG SmlI 351 CTCAAG C^T[CT][GA]AG StuI 952 AGGCCT AGG^CCT TaqI 864 TCGA T^CGA TthHB8I 864 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AfaI 83 GTAC GT^AC 498 GTAC AhdI 619 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1199 GACCAGAAGTC AspEI 619 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1199 GACCAGAAGTC AviII 36 TGCGCA TGC^GCA 1014 TGCGCA BalI 390 TGGCCA TGG^CCA 567 TGGCCA BbeI 144 GGCGCC GGCGC^C 907 GGCGCC BbsI 47 GTCTTC GAAGAC(2/6) 1211 GTCTTC BcnI 511 CCCGG CC^[GC]GG 1283 CCCGG BfaI 1566 CTAG C^TAG 1600 CTAG BpuAI 47 GTCTTC GAAGAC(2/6) 1211 GTCTTC BsaI 120 GAGACC GGTCTC(1/5) 1520 GAGACC BseRI 344 GAGGAG GAGGAG(10/8) 815 GAGGAG BstAPI 437 GCATTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1232 GCACTGACTGC BstXI 72 CCATGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1326 CCATCCTCCTGG DraII 168 GGGACCT [GA]G^G[ACGT]CC[CT] 1008 AGGGCCT DsaI 72 CCATGG C^C[GA][CT]GG 1540 CCGCGG Eam1105I 619 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1199 GACCAGAAGTC EcoO109I 168 GGGACCT [GA]G^G[ACGT]CC[CT] 1008 AGGGCCT FspI 36 TGCGCA TGC^GCA 1014 TGCGCA KasI 144 GGCGCC G^GCGCC 907 GGCGCC MaeI 1566 CTAG C^TAG 1600 CTAG MluNI 390 TGGCCA TGG^CCA 567 TGGCCA MscI 390 TGGCCA TGG^CCA 567 TGGCCA MspA1I 1511 CAGCTG C[AC]G^C[GT]G 1540 CCGCGG NarI 144 GGCGCC GG^CGCC 907 GGCGCC NciI 511 CCCGG CC^[GC]GG 1283 CCCGG PflMI 1051 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1131 CCATCGTCTGG RsaI 83 GTAC GT^AC 498 GTAC SfoI 144 GGCGCC GGC^GCC 907 GGCGCC Van91I 1051 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1131 CCATCGTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AcyI AluI AvaII BanI BanII BbvI BglII BmyI BpmI BsaHI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp45I Tsp509I TspRI XcmI XhoII