##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AclI AcyI AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlnI BpuAI
BsaAI BsaHI BseRI BsgI BsiEI
BsiWI BsoBI BspDI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KspI
MaeII MfeI MunI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 306 GTAGAC GT^[AC][GT]AC
AccII 5 CGCG CG^CG
AflII 1169 CTTAAG C^TTAAG
AlwNI 237 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 827 GGGCCC GGGCC^C
BalI 772 TGGCCA TGG^CCA
BanII 827 GGGCCC G[GA]GC[CT]^C
BfrI 1169 CTTAAG C^TTAAG
BlpI 1346 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1346 GCTGAGC GC^T[ACGT]AGC
BsaI 274 GAGACC GGTCTC(1/5)
BsmBI 135 GAGACG CGTCTC(1/5)
BsmI 895 GCATTC GAATGC(1/-1)
Bsp1407I 939 TGTACA T^GTACA
BspHI 1269 TCATGA T^CATGA
BspLU11I 715 ACATGT A^CATGT
BsrGI 939 TGTACA T^GTACA
BstUI 5 CGCG CG^CG
CelII 1346 GCTGAGC GC^T[ACGT]AGC
DraIII 1394 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 769 CCGTGG C^C[GA][CT]GG
EaeI 772 TGGCCA [CT]^GGCC[GA]
EarI 201 GAAGAG CTCTTC(1/4)
EcoRI 102 GAATTC G^AATTC
EcoRV 1235 GATATC GAT^ATC
EcoT14I 823 CCTTGG C^C[AT][AT]GG
HgaI 6 GCGTC GACGC(5/10)
HindIII 153 AAGCTT A^AGCTT
Ksp632I 201 GAAGAG CTCTTC(1/4)
MluI 4 ACGCGT A^CGCGT
MluNI 772 TGGCCA TGG^CCA
MscI 772 TGGCCA TGG^CCA
MvnI 5 CGCG CG^CG
NdeI 915 CATATG CA^TATG
PshAI 1442 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1269 TCATGA T^CATGA
SexAI 301 ACCTGGT A^CC[AT]GGT
SspBI 939 TGTACA T^GTACA
StyI 823 CCTTGG C^C[AT][AT]GG
TaqI 562 TCGA T^CGA
TfiI 1415 GAATC G^A[AT]TC
Tsp45I 663 GTGAC ^GT[GC]AC
TthHB8I 562 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 162 GGTACC G^GTACC
1094 GGTACC
AccIII 325 TCCGGA T^CCGGA
730 TCCGGA
AcsI 102 GAATTC [GA]^AATT[CT]
1084 GAATTT
AflIII 4 ACGCGT A^C[GA][CT]GT
715 ACATGT
AlwI 511 GATCC GGATC(4/5)
865 GGATC
ApoI 102 GAATTC [GA]^AATT[CT]
1084 GAATTT
Asp718I 162 GGTACC G^GTACC
1094 GGTACC
AspHI 329 GAGCAC G[AT]GC[AT]^C
1075 GTGCTC
BcnI 518 CCCGG CC^[GC]GG
650 CCGGG
BsaBI 53 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1235 GATATCTATC
BsaWI 325 TCCGGA [AT]^CCGG[AT]
730 TCCGGA
BseAI 325 TCCGGA T^CCGGA
730 TCCGGA
BsiHKAI 329 GAGCAC G[AT]GC[AT]^C
1075 GTGCTC
BsmFI 1311 GTCCC GGGAC(10/14)
1449 GTCCC
BspEI 325 TCCGGA T^CCGGA
730 TCCGGA
BsrDI 314 CATTGC GCAATG(2/0)
463 CATTGC
BstAPI 419 GCAACTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1068 GCATCCAGTGC
BstYI 510 AGATCC [GA]^GATC[CT]
865 GGATCT
DpnII 511 GATC ^GATC
866 GATC
KpnI 162 GGTACC GGTAC^C
1094 GGTACC
MaeIII 663 GTGAC ^GT[ACGT]AC
1013 GTTAC
MamI 53 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1235 GATATCTATC
MboI 511 GATC ^GATC
866 GATC
MflI 510 AGATCC [GA]^GATC[CT]
865 GGATCT
MroI 325 TCCGGA T^CCGGA
730 TCCGGA
MslI 1197 CATGACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1270 CATGAGAGTG
MspA1I 193 CAGCTG C[AC]G^C[GT]G
246 CAGCTG
NciI 518 CCCGG CC^[GC]GG
650 CCGGG
NdeII 511 GATC ^GATC
866 GATC
PleI 790 GACTC GAGTC(4/5)
1442 GACTC
PstI 243 CTGCAG CTGCA^G
546 CTGCAG
PvuII 193 CAGCTG CAG^CTG
246 CAGCTG
Sau3AI 511 GATC ^GATC
866 GATC
XcmI 292 CCAGTGATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1080 CCACGAATTTAGTGG
XhoII 510 AGATCC [GA]^GATC[CT]
865 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbvI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MboII MnlI MseI MspI
MvaI MwoI NlaIII NlaIV NspI
RsaI Sau96I ScrFI SfaNI SfcI
SmlI Tru9I TseI Tsp509I TspRI