################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BglI BglII BlnI BpuAI BsaAI BsaHI BseRI BsgI BsiEI BsiWI BsoBI BspDI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI KasI KspI MaeII MfeI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 306 GTAGAC GT^[AC][GT]AC AccII 5 CGCG CG^CG AflII 1169 CTTAAG C^TTAAG AlwNI 237 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 827 GGGCCC GGGCC^C BalI 772 TGGCCA TGG^CCA BanII 827 GGGCCC G[GA]GC[CT]^C BfrI 1169 CTTAAG C^TTAAG BlpI 1346 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1346 GCTGAGC GC^T[ACGT]AGC BsaI 274 GAGACC GGTCTC(1/5) BsmBI 135 GAGACG CGTCTC(1/5) BsmI 895 GCATTC GAATGC(1/-1) Bsp1407I 939 TGTACA T^GTACA BspHI 1269 TCATGA T^CATGA BspLU11I 715 ACATGT A^CATGT BsrGI 939 TGTACA T^GTACA BstUI 5 CGCG CG^CG CelII 1346 GCTGAGC GC^T[ACGT]AGC DraIII 1394 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 769 CCGTGG C^C[GA][CT]GG EaeI 772 TGGCCA [CT]^GGCC[GA] EarI 201 GAAGAG CTCTTC(1/4) EcoRI 102 GAATTC G^AATTC EcoRV 1235 GATATC GAT^ATC EcoT14I 823 CCTTGG C^C[AT][AT]GG HgaI 6 GCGTC GACGC(5/10) HindIII 153 AAGCTT A^AGCTT Ksp632I 201 GAAGAG CTCTTC(1/4) MluI 4 ACGCGT A^CGCGT MluNI 772 TGGCCA TGG^CCA MscI 772 TGGCCA TGG^CCA MvnI 5 CGCG CG^CG NdeI 915 CATATG CA^TATG PshAI 1442 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1269 TCATGA T^CATGA SexAI 301 ACCTGGT A^CC[AT]GGT SspBI 939 TGTACA T^GTACA StyI 823 CCTTGG C^C[AT][AT]GG TaqI 562 TCGA T^CGA TfiI 1415 GAATC G^A[AT]TC Tsp45I 663 GTGAC ^GT[GC]AC TthHB8I 562 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 162 GGTACC G^GTACC 1094 GGTACC AccIII 325 TCCGGA T^CCGGA 730 TCCGGA AcsI 102 GAATTC [GA]^AATT[CT] 1084 GAATTT AflIII 4 ACGCGT A^C[GA][CT]GT 715 ACATGT AlwI 511 GATCC GGATC(4/5) 865 GGATC ApoI 102 GAATTC [GA]^AATT[CT] 1084 GAATTT Asp718I 162 GGTACC G^GTACC 1094 GGTACC AspHI 329 GAGCAC G[AT]GC[AT]^C 1075 GTGCTC BcnI 518 CCCGG CC^[GC]GG 650 CCGGG BsaBI 53 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1235 GATATCTATC BsaWI 325 TCCGGA [AT]^CCGG[AT] 730 TCCGGA BseAI 325 TCCGGA T^CCGGA 730 TCCGGA BsiHKAI 329 GAGCAC G[AT]GC[AT]^C 1075 GTGCTC BsmFI 1311 GTCCC GGGAC(10/14) 1449 GTCCC BspEI 325 TCCGGA T^CCGGA 730 TCCGGA BsrDI 314 CATTGC GCAATG(2/0) 463 CATTGC BstAPI 419 GCAACTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1068 GCATCCAGTGC BstYI 510 AGATCC [GA]^GATC[CT] 865 GGATCT DpnII 511 GATC ^GATC 866 GATC KpnI 162 GGTACC GGTAC^C 1094 GGTACC MaeIII 663 GTGAC ^GT[ACGT]AC 1013 GTTAC MamI 53 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1235 GATATCTATC MboI 511 GATC ^GATC 866 GATC MflI 510 AGATCC [GA]^GATC[CT] 865 GGATCT MroI 325 TCCGGA T^CCGGA 730 TCCGGA MslI 1197 CATGACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1270 CATGAGAGTG MspA1I 193 CAGCTG C[AC]G^C[GT]G 246 CAGCTG NciI 518 CCCGG CC^[GC]GG 650 CCGGG NdeII 511 GATC ^GATC 866 GATC PleI 790 GACTC GAGTC(4/5) 1442 GACTC PstI 243 CTGCAG CTGCA^G 546 CTGCAG PvuII 193 CAGCTG CAG^CTG 246 CAGCTG Sau3AI 511 GATC ^GATC 866 GATC XcmI 292 CCAGTGATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1080 CCACGAATTTAGTGG XhoII 510 AGATCC [GA]^GATC[CT] 865 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AfaI AluI AvaII BanI BbvI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MboII MnlI MseI MspI MvaI MwoI NlaIII NlaIV NspI RsaI Sau96I ScrFI SfaNI SfcI SmlI Tru9I TseI Tsp509I TspRI