##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MunI MvnI NarI NcoI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Van91I XbaI
XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 208 GTCTAC GT^[AC][GT]AC
AfaI 139 GTAC GT^AC
AspI 511 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 352 CCCGGG C^[CT]CG[GA]G
BbvCI 903 CCTCAGC CCTCAGC(-5/-2)
BcgI 551 CGAAGCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 415 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1020 GAGACC GGTCTC(1/5)
BsmI 812 GCATTC GAATGC(1/-1)
BsoBI 352 CCCGGG C^[CT]CG[GA]G
BspLU11I 2 ACATGT A^CATGT
BsrDI 942 CATTGC GCAATG(2/0)
BsrFI 920 GCCGGC [GA]^CCGG[CT]
BstAPI 819 GCAGTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 920 GCCGGC [GA]^CCGG[CT]
DpnII 39 GATC ^GATC
DsaI 530 CCGTGG C^C[GA][CT]GG
FauI 476 CCCGC CCCGC(4/6)
HaeII 98 AGCGCC [GA]GCGC^[CT]
HincII 658 GTCAAC GT[CT]^[GA]AC
HindII 658 GTCAAC GT[CT]^[GA]AC
HphI 852 GGTGA GGTGA(8/7)
MboI 39 GATC ^GATC
MspA1I 734 CCGCTG C[AC]G^C[GT]G
NaeI 920 GCCGGC GCC^GGC
NdeI 196 CATATG CA^TATG
NdeII 39 GATC ^GATC
NgoMIV 920 GCCGGC G^CCGGC
PflFI 511 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 139 GTAC GT^AC
Sau3AI 39 GATC ^GATC
SmaI 352 CCCGGG CCC^GGG
TaqI 550 TCGA T^CGA
Tsp509I 1081 AATT ^AATT
Tth111I 511 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 550 TCGA T^CGA
XmaCI 352 CCCGGG C^CCGGG
XmaI 352 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 205 GGCGTC G[GA]^CG[CT]C
468 GGCGTC
AflIII 2 ACATGT A^C[GA][CT]GT
266 ACACGT
BcnI 352 CCCGG CC^[GC]GG
353 CCGGG
BsaHI 205 GGCGTC G[GA]^CG[CT]C
468 GGCGTC
BseRI 772 GAGGAG GAGGAG(10/8)
956 GAGGAG
BsgI 947 CTGCAC GTGCAG(16/14)
757 GTGCAG
BspMI 83 ACCTGC ACCTGC(4/8)
924 GCAGGT
BsrBI 165 CCGCTC CCGCTC(-3/-3)
615 CCGCTC
BstXI 53 CCACAGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
134 CCAAGGTACTGG
CfoI 99 GCGC GCG^C
409 GCGC
EcoNI 228 CCTGATTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
789 CCTGGAGCAGG
EcoT14I 134 CCAAGG C^C[AT][AT]GG
878 CCAAGG
HapII 353 CCGG C^CGG
921 CCGG
HgaI 206 GCGTC GACGC(5/10)
469 GCGTC
HhaI 99 GCGC GCG^C
409 GCGC
Hin1I 205 GGCGTC G[GA]^CG[CT]C
468 GGCGTC
HinP1I 99 GCGC G^CGC
409 GCGC
HpaII 353 CCGG C^CGG
921 CCGG
MaeII 14 ACGT A^CGT
268 ACGT
MseI 233 TTAA T^TAA
271 TTAA
MslI 1 CACATGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
54 CACAGGCGTG
MspI 353 CCGG C^CGG
921 CCGG
NciI 352 CCCGG CC^[GC]GG
353 CCGGG
PshAI 324 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
464 GACAGGCGTC
PstI 817 CTGCAG CTGCA^G
973 CTGCAG
SfcI 817 CTGCAG C^T[GA][CT]AG
973 CTGCAG
StyI 134 CCAAGG C^C[AT][AT]GG
878 CCAAGG
Tru9I 233 TTAA T^TAA
271 TTAA
XcmI 296 CCATCTTCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
878 CCAAGGTTGTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AspHI AvaII BalI
BanII BbvI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII EaeI
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MaeIII
MboII MluNI MnlI MscI MvaI
MwoI NlaIII NlaIV NspI PleI
PpuMI SacI Sau96I ScrFI SfaNI
TseI Tsp45I TspRI