##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AlwI AlwNI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbvCI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BseAI BsiWI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstXI BstYI BstZ17I
CelII Cfr10I CpoI DraI Eam1105I
Eco47III EcoO65I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XhoII XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 25 GGCGCC G[GA]^CG[CT]C
AflIII 149 ACACGT A^C[GA][CT]GT
AocI 1171 CCTCAGG CC^T[ACGT]AGG
BbeI 25 GGCGCC GGCGC^C
BcgI 462 CGATGAATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 403 GGATAC GTATCC(6/5)
BsaHI 25 GGCGCC G[GA]^CG[CT]C
BsaWI 1136 ACCGGA [AT]^CCGG[AT]
BsgI 576 CTGCAC GTGCAG(16/14)
BsiEI 375 CGGCCG CG[GA][CT]^CG
BsmBI 1195 GAGACG CGTCTC(1/5)
BspDI 460 ATCGAT AT^CGAT
BstAPI 687 GCAGACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1094 TTCGAA TT^CGAA
Bsu36I 1171 CCTCAGG CC^T[ACGT]AGG
ClaI 460 ATCGAT AT^CGAT
DdeI 1172 CTCAG C^T[ACGT]AG
DpnII 210 GATC ^GATC
DraIII 255 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 443 GACCGTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 375 CGGCCG [CT]^GGCC[GA]
EagI 375 CGGCCG C^GGCCG
EclXI 375 CGGCCG C^GGCCG
Eco52I 375 CGGCCG C^GGCCG
Eco81I 1171 CCTCAGG CC^T[ACGT]AGG
EcoRI 1177 GAATTC G^AATTC
EcoRV 457 GATATC GAT^ATC
FauI 435 GCGGG CCCGC(4/6)
HgaI 1197 GACGC GACGC(5/10)
Hin1I 25 GGCGCC G[GA]^CG[CT]C
KasI 25 GGCGCC G^GCGCC
MaeIII 764 GTGAC ^GT[ACGT]AC
MboI 210 GATC ^GATC
MseI 950 TTAA T^TAA
NarI 25 GGCGCC GG^CGCC
NcoI 515 CCATGG C^CATGG
NdeII 210 GATC ^GATC
NspI 661 ACATGC [GA]CATG^[CT]
NspV 1094 TTCGAA TT^CGAA
PleI 1140 GACTC GAGTC(4/5)
Sau3AI 210 GATC ^GATC
SfaNI 506 GCATC GCATC(5/9)
SfoI 25 GGCGCC GGC^GCC
SfuI 1094 TTCGAA TT^CGAA
SmlI 556 CTTGAG C^T[CT][GA]AG
TfiI 863 GATTC G^A[AT]TC
Tru9I 950 TTAA T^TAA
Tsp45I 764 GTGAC ^GT[GC]AC
XcmI 92 CCAAGAGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 447 GTAC GT^AC
772 GTAC
BanI 25 GGCGCC G^G[CT][GA]CC
57 GGTGCC
BbsI 995 GAAGAC GAAGAC(2/6)
240 GTCTTC
BpuAI 995 GAAGAC GAAGAC(2/6)
240 GTCTTC
BsaI 441 GAGACC GGTCTC(1/5)
1040 GGTCTC
BseRI 83 GAGGAG GAGGAG(10/8)
1019 GAGGAG
BspMI 574 ACCTGC ACCTGC(4/8)
649 ACCTGC
BstF5I 552 CATCC GGATG(2/0)
1284 CATCC
EcoNI 575 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1167 CCTACCTCAGG
FokI 552 CATCC GGATG(9/13)
1284 CATCC
HinfI 863 GATTC G^A[ACGT]TC
1140 GACTC
PstI 697 CTGCAG CTGCA^G
1114 CTGCAG
PvuII 100 CAGCTG CAG^CTG
700 CAGCTG
RsaI 447 GTAC GT^AC
772 GTAC
SacI 18 GAGCTC GAGCT^C
250 GAGCTC
SfcI 697 CTGCAG C^T[GA][CT]AG
1114 CTGCAG
SfiI 958 GGCCGGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
967 GGCCCCGTGGGCC
SmaI 399 CCCGGG CCC^GGG
1035 CCCGGG
XmaCI 399 CCCGGG C^CCGGG
1035 CCCGGG
XmaI 399 CCCGGG C^CCGGG
1035 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcsI AluI Alw44I
ApaI ApaLI ApoI AspHI AvaI
AvaII BanII BbrPI BbvI BcnI
BglI BmyI BpmI BsaAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DraII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PmaCI PmlI PpuMI Sau96I
ScrFI StyI TaqI TseI Tsp509I
TspRI TthHB8I