##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BbeI BbrPI
BbvCI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI Hin1I HincII HindII HpaI
HpaII KasI KpnI KspI MfeI
MluI MroI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XcmI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AciI 1086 GCGG CCGC(-3/-1)
AcsI 440 GAATTT [GA]^AATT[CT]
AflIII 492 ACATGT A^C[GA][CT]GT
AlwNI 669 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 440 GAATTT [GA]^AATT[CT]
AseI 992 ATTAAT AT^TAAT
AsnI 992 ATTAAT AT^TAAT
AvaI 1006 CTCGAG C^[CT]CG[GA]G
BalI 388 TGGCCA TGG^CCA
BamHI 74 GGATCC G^GATCC
BanI 970 GGTGCC G^G[CT][GA]CC
BbsI 782 GTCTTC GAAGAC(2/6)
BcgI 1275 GCACAGGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 1184 CTGGAG CTGGAG(16/14)
BpuAI 782 GTCTTC GAAGAC(2/6)
BsaBI 69 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 917 GGTCTC GGTCTC(1/5)
BsoBI 1006 CTCGAG C^[CT]CG[GA]G
BspLU11I 492 ACATGT A^CATGT
BsrDI 685 GCAATG GCAATG(2/0)
BstAPI 873 GCACACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 391 CCAAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1075 GCGC GCG^C
DraII 288 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 874 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 750 GACTCTAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 288 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1327 CCAAGG C^C[AT][AT]GG
HhaI 1075 GCGC GCG^C
HinP1I 1075 GCGC G^CGC
HindIII 551 AAGCTT A^AGCTT
MamI 69 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 388 TGGCCA TGG^CCA
MscI 388 TGGCCA TGG^CCA
MslI 104 CATCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 1006 CTCGAG C^TCGAG
PshBI 992 ATTAAT AT^TAAT
PstI 796 CTGCAG CTGCA^G
SexAI 561 ACCTGGT A^CC[AT]GGT
StyI 1327 CCAAGG C^C[AT][AT]GG
TaqI 1007 TCGA T^CGA
TthHB8I 1007 TCGA T^CGA
XbaI 632 TCTAGA T^CTAGA
XhoI 1006 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 116 GTAC GT^AC
1059 GTAC
AspHI 90 GTGCTC G[AT]GC[AT]^C
1273 GAGCAC
BanII 831 GAGCCC G[GA]GC[CT]^C
860 GAGCCC
BsiHKAI 90 GTGCTC G[AT]GC[AT]^C
1273 GAGCAC
BsmBI 894 CGTCTC CGTCTC(1/5)
1009 GAGACG
BsrI 52 ACTGG ACTGG(1/-1)
930 ACTGG
BstYI 68 GGATCT [GA]^GATC[CT]
74 GGATCC
Cac8I 640 GCTTGC GC[ACGT]^[ACGT]GC
668 GCAGGC
Cfr13I 289 GGGCC G^G[ACGT]CC
537 GGCCC
DsaI 34 CCGTGG C^C[GA][CT]GG
700 CCGTGG
EaeI 31 TGGCCG [CT]^GGCC[GA]
388 TGGCCA
EarI 1017 GAAGAG CTCTTC(1/4)
1203 GAAGAG
HphI 179 GGTGA GGTGA(8/7)
1102 TCACC
Ksp632I 1017 GAAGAG CTCTTC(1/4)
1203 GAAGAG
MaeII 824 ACGT A^CGT
904 ACGT
MflI 68 GGATCT [GA]^GATC[CT]
74 GGATCC
NspI 492 ACATGT [GA]CATG^[CT]
1077 GCATGT
RsaI 116 GTAC GT^AC
1059 GTAC
Sau96I 289 GGGCC G^G[ACGT]CC
537 GGCCC
SfcI 418 CTACAG C^T[GA][CT]AG
796 CTGCAG
SmlI 897 CTCAAG C^T[CT][GA]AG
1006 CTCGAG
Tsp45I 576 GTCAC ^GT[GC]AC
1101 GTCAC
XhoII 68 GGATCT [GA]^GATC[CT]
74 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AluI AlwI BbvI BfaI BmyI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BssKI
BstF5I BstNI DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI ScrFI SfaNI
TfiI Tru9I TseI Tsp509I TspRI