##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII AccI AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
AspEI AspI AviII AvrII BamHI
BbrPI BcgI BciVI BclI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsiWI Bsp1407I BspDI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KspI MamI MfeI MluI MunI
NaeI NdeI NgoMIV NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I Van91I XbaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 799 GGTACC G^GTACC
AccII 1703 CGCG CG^CG
AccIII 53 TCCGGA T^CCGGA
AclI 819 AACGTT AA^CGTT
AflII 465 CTTAAG C^TTAAG
AflIII 1098 ACATGT A^C[GA][CT]GT
Alw44I 1146 GTGCAC G^TGCAC
AlwI 1541 GATCC GGATC(4/5)
ApaLI 1146 GTGCAC G^TGCAC
Asp718I 799 GGTACC G^GTACC
AvaII 961 GGTCC G^G[AT]CC
BalI 1582 TGGCCA TGG^CCA
BbeI 122 GGCGCC GGCGC^C
BbvCI 1552 CCTCAGC CCTCAGC(-5/-2)
BfrI 465 CTTAAG C^TTAAG
BglII 841 AGATCT A^GATCT
BsaWI 53 TCCGGA [AT]^CCGG[AT]
BseAI 53 TCCGGA T^CCGGA
BsiEI 1576 CGGCCG CG[GA][CT]^CG
BsmI 1160 GCATTC GAATGC(1/-1)
BspEI 53 TCCGGA T^CCGGA
BspHI 968 TCATGA T^CATGA
BspLU11I 1098 ACATGT A^CATGT
BssSI 246 CACGAG CACGAG(-5/-1)
BstUI 1703 CGCG CG^CG
BstXI 1271 CCAGTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 1576 CGGCCG C^GGCCG
EclXI 1576 CGGCCG C^GGCCG
Eco52I 1576 CGGCCG C^GGCCG
EcoRI 323 GAATTC G^AATTC
HindIII 1370 AAGCTT A^AGCTT
KasI 122 GGCGCC G^GCGCC
KpnI 799 GGTACC GGTAC^C
MaeII 820 ACGT A^CGT
MluNI 1582 TGGCCA TGG^CCA
MroI 53 TCCGGA T^CCGGA
MscI 1582 TGGCCA TGG^CCA
MvnI 1703 CGCG CG^CG
NarI 122 GGCGCC GG^CGCC
NheI 212 GCTAGC G^CTAGC
PshAI 455 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 819 AACGTT AA^CGTT
PstI 1609 CTGCAG CTGCA^G
RcaI 968 TCATGA T^CATGA
SacI 156 GAGCTC GAGCT^C
ScaI 1303 AGTACT AGT^ACT
SfcI 1609 CTGCAG C^T[GA][CT]AG
SfoI 122 GGCGCC GGC^GCC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 122 GGCGCC G[GA]^CG[CT]C
455 GACGCC
AlwNI 286 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1603 CAGCCCCTG
AvaI 655 CTCGAG C^[CT]CG[GA]G
1646 CTCGAG
BanII 156 GAGCTC G[GA]GC[CT]^C
1622 GAGCCC
BcnI 317 CCGGG CC^[GC]GG
601 CCCGG
BfaI 213 CTAG C^TAG
1859 CTAG
BsaHI 122 GGCGCC G[GA]^CG[CT]C
455 GACGCC
BsaI 1104 GAGACC GGTCTC(1/5)
1649 GAGACC
BsmBI 249 GAGACG CGTCTC(1/5)
453 GAGACG
BsoBI 655 CTCGAG C^[CT]CG[GA]G
1646 CTCGAG
BspMI 1675 ACCTGC ACCTGC(4/8)
1735 ACCTGC
BstAPI 75 GCAGTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1148 GCACTCCTTGC
BstYI 841 AGATCT [GA]^GATC[CT]
1540 AGATCC
EaeI 1576 CGGCCG [CT]^GGCC[GA]
1582 TGGCCA
HaeII 122 GGCGCC [GA]GCGC^[CT]
719 AGCGCC
HgaI 455 GACGC GACGC(5/10)
1325 GACGC
Hin1I 122 GGCGCC G[GA]^CG[CT]C
455 GACGCC
MaeI 213 CTAG C^TAG
1859 CTAG
MflI 841 AGATCT [GA]^GATC[CT]
1540 AGATCC
NciI 317 CCGGG CC^[GC]GG
601 CCCGG
PaeR7I 655 CTCGAG C^TCGAG
1646 CTCGAG
PvuII 1531 CAGCTG CAG^CTG
1561 CAGCTG
SfaNI 1240 GCATC GCATC(5/9)
1691 GCATC
SphI 80 GCATGC GCATG^C
835 GCATGC
XhoI 655 CTCGAG C^TCGAG
1646 CTCGAG
XhoII 841 AGATCT [GA]^GATC[CT]
1540 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AspHI BanI BbsI BbvI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SmlI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI