##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AviII AvrII BalI BbeI BbrPI
BbsI BcgI BciVI BfrI BglI
BlnI BpuAI BsaAI BsaHI BseAI
BseRI BsgI BsiHKAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HgaI Hin1I HindIII HpaI KasI
MaeII MfeI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuII RcaI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tru9I Tsp509I
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 1176 GTCTAC GT^[AC][GT]AC
AccII 1141 CGCG CG^CG
AlwNI 1283 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 805 AGCGCT AGC^GCT
ApaI 493 GGGCCC GGGCC^C
BclI 600 TGATCA T^GATCA
BglII 403 AGATCT A^GATCT
BlpI 558 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 558 GCTCAGC GC^T[ACGT]AGC
BsaBI 1429 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1385 ACCGGA [AT]^CCGG[AT]
BsiEI 1299 CGATCG CG[GA][CT]^CG
Bsp1407I 1431 TGTACA T^GTACA
BsrBI 1424 GAGCGG CCGCTC(-3/-3)
BsrGI 1431 TGTACA T^GTACA
BstUI 1141 CGCG CG^CG
BstXI 93 CCAGCCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 558 GCTCAGC GC^T[ACGT]AGC
CfoI 806 GCGC GCG^C
DraII 493 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 1140 CCGCGG C^C[GA][CT]GG
Eco47III 805 AGCGCT AGC^GCT
EcoO109I 493 GGGCCCT [GA]G^G[ACGT]CC[CT]
FbaI 600 TGATCA T^GATCA
HaeII 805 AGCGCT [GA]GCGC^[CT]
HhaI 806 GCGC GCG^C
HinP1I 806 GCGC G^CGC
HincII 1294 GTCAAC GT[CT]^[GA]AC
HindII 1294 GTCAAC GT[CT]^[GA]AC
KspI 1140 CCGCGG CCGC^GG
MamI 1429 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1140 CCGCGG C[AC]G^C[GT]G
MvnI 1141 CGCG CG^CG
NspI 707 ACATGC [GA]CATG^[CT]
PflMI 1327 CCAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 435 GACTC GAGTC(4/5)
PvuI 1299 CGATCG CGAT^CG
SacII 1140 CCGCGG CCGC^GG
SmlI 387 CTTGAG C^T[CT][GA]AG
SspBI 1431 TGTACA T^GTACA
StuI 1234 AGGCCT AGG^CCT
Van91I 1327 CCAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1227 CCACACTAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Acc65I 249 GGTACC G^GTACC
393 GGTACC
Asp718I 249 GGTACC G^GTACC
393 GGTACC
AvaI 27 CCCGGG C^[CT]CG[GA]G
253 CCCGGG
BanI 249 GGTACC G^G[CT][GA]CC
393 GGTACC
BbvCI 1181 CCTCAGC CCTCAGC(-5/-2)
1027 GCTGAGG
BpmI 437 CTCCAG CTGGAG(16/14)
1421 CTGGAG
BsaI 364 GAGACC GGTCTC(1/5)
1166 GGTCTC
BsoBI 27 CCCGGG C^[CT]CG[GA]G
253 CCCGGG
EcoNI 834 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
945 CCTGACAGAGG
FauI 467 CCCGC CCCGC(4/6)
859 CCCGC
KpnI 249 GGTACC GGTAC^C
393 GGTACC
MaeIII 310 GTCAC ^GT[ACGT]AC
1322 GTCAC
SapI 1270 GAAGAGC GCTCTTC(1/4)
926 GCTCTTC
SmaI 27 CCCGGG CCC^GGG
253 CCCGGG
Tsp45I 310 GTCAC ^GT[GC]AC
1322 GTCAC
XmaCI 27 CCCGGG C^CCGGG
253 CCCGGG
XmaI 27 CCCGGG C^CCGGG
253 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BamHI BanII BbvI BcnI BfaI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MboI
MboII MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
TseI TspRI TthHB8I XhoII