##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI AspEI AspI AviII
AvrII BanII BbeI BbrPI BcgI
BclI BlnI BsaAI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrFI BssHII BstBI
Cfr10I DraI DraIII EagI Eam1105I
EclXI Eco52I EcoNI FbaI FseI
FspI Hin1I HincII HindII HpaI
KasI KspI MroI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 1871 GTATAC GT^[AC][GT]AC
AflII 2900 CTTAAG C^TTAAG
AlwNI 2533 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3808 CCTTAGG CC^T[ACGT]AGG
Asp700I 1344 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 750 GAGCAC G[AT]GC[AT]^C
BalI 2551 TGGCCA TGG^CCA
BciVI 1758 GGATAC GTATCC(6/5)
BcnI 1848 CCCGG CC^[GC]GG
BfrI 2900 CTTAAG C^TTAAG
BglI 1795 GCCTAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 4179 CTCCAG CTGGAG(16/14)
BsaBI 2807 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 4020 CTCCTC GAGGAG(10/8)
BsiHKAI 750 GAGCAC G[AT]GC[AT]^C
Bsp1407I 164 TGTACA T^GTACA
BspDI 1908 ATCGAT AT^CGAT
BspLU11I 3838 ACATGT A^CATGT
BsrGI 164 TGTACA T^GTACA
BssSI 4650 CACGAG CACGAG(-5/-1)
Bst1107I 1871 GTATAC GTA^TAC
BstEII 681 GGTCACC G^GT[ACGT]ACC
BstPI 681 GGTCACC G^GT[ACGT]ACC
BstZ17I 1871 GTATAC GTA^TAC
Bsu36I 3808 CCTTAGG CC^T[ACGT]AGG
ClaI 1908 ATCGAT AT^CGAT
CpoI 4397 CGGACCG CG^G[AT]CCG
DraII 2306 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 3979 GACCAGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2551 TGGCCA [CT]^GGCC[GA]
Eco81I 3808 CCTTAGG CC^T[ACGT]AGG
EcoO109I 2306 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 681 GGTCACC G^GT[ACGT]ACC
EcoRV 2996 GATATC GAT^ATC
FauI 3873 CCCGC CCCGC(4/6)
HapII 1849 CCGG C^CGG
HgaI 4618 GCGTC GACGC(5/10)
HpaII 1849 CCGG C^CGG
MaeII 643 ACGT A^CGT
MamI 2807 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 4616 ACGCGT A^CGCGT
MluNI 2551 TGGCCA TGG^CCA
MscI 2551 TGGCCA TGG^CCA
MspI 1849 CCGG C^CGG
NciI 1848 CCCGG CC^[GC]GG
PflMI 3590 CCACTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2306 GGGACCC [GA]G^G[AT]CC[CT]
RsrII 4397 CGGACCG CG^G[AT]CCG
SspBI 164 TGTACA T^GTACA
Van91I 3590 CCACTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1344 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 1675 GGTACC G^GTACC
3575 GGTACC
AccII 3848 CGCG CG^CG
4617 CGCG
AflIII 3838 ACATGT A^C[GA][CT]GT
4616 ACGCGT
Aor51HI 3108 AGCGCT AGC^GCT
4632 AGCGCT
Asp718I 1675 GGTACC G^GTACC
3575 GGTACC
AvaI 3132 CTCGGG C^[CT]CG[GA]G
3801 CCCGAG
BamHI 1957 GGATCC G^GATCC
4091 GGATCC
BanI 1675 GGTACC G^G[CT][GA]CC
3575 GGTACC
BbvCI 4022 CCTCAGC CCTCAGC(-5/-2)
4028 CCTCAGC
BglII 1075 AGATCT A^GATCT
3222 AGATCT
BmyI 284 GTGCCC G[AGT]GC[ACT]^C
750 GAGCAC
BsaI 3804 GAGACC GGTCTC(1/5)
822 GGTCTC
BsoBI 3132 CTCGGG C^[CT]CG[GA]G
3801 CCCGAG
Bsp1286I 284 GTGCCC G[AGT]GC[ACT]^C
750 GAGCAC
BspMI 2080 ACCTGC ACCTGC(4/8)
3934 GCAGGT
BsrDI 14 CATTGC GCAATG(2/0)
3466 CATTGC
BstUI 3848 CGCG CG^CG
4617 CGCG
Eco47III 3108 AGCGCT AGC^GCT
4632 AGCGCT
EcoRI 1098 GAATTC G^AATTC
4045 GAATTC
HindIII 2178 AAGCTT A^AGCTT
4103 AAGCTT
KpnI 1675 GGTACC GGTAC^C
3575 GGTACC
MvnI 3848 CGCG CG^CG
4617 CGCG
NheI 1288 GCTAGC G^CTAGC
3040 GCTAGC
NspI 3838 ACATGT [GA]CATG^[CT]
4489 ACATGC
SapI 3293 GAAGAGC GCTCTTC(1/4)
4064 GCTCTTC
SmlI 2900 CTTAAG C^T[CT][GA]AG
4596 CTCAAG
SspI 458 AATATT AAT^ATT
788 AATATT
XbaI 646 TCTAGA T^CTAGA
3518 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BbvI BfaI
BlpI Bpu1102I BpuAI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsmI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DsaI EarI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeII HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MunI MvaI
MwoI NcoI NdeI NdeII NlaIII
NlaIV NsiI PleI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII