##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccI AclI AflII AgeI AscI
AseI AsnI AviII AvrII BamHI
BbeI BbsI BfrI BlnI BpuAI
BsaBI BsiEI BsiWI BspDI BspHI
BspLU11I BssHII Bst1107I BstAPI BstZ17I
ClaI CpoI DraI EagI EclXI
Eco52I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HpaI KasI
KspI MamI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SnaBI SpeI Sse8387I
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 1252 GGTACC G^GTACC
AccII 1459 CGCG CG^CG
AccIII 2174 TCCGGA T^CCGGA
AflIII 1458 ACGCGT A^C[GA][CT]GT
Alw44I 1487 GTGCAC G^TGCAC
Aor51HI 3439 AGCGCT AGC^GCT
ApaLI 1487 GTGCAC G^TGCAC
Asp718I 1252 GGTACC G^GTACC
BbrPI 2751 CACGTG CAC^GTG
BclI 201 TGATCA T^GATCA
BsaAI 2751 CACGTG [CT]AC^GT[GA]
BsaWI 2174 TCCGGA [AT]^CCGG[AT]
BseAI 2174 TCCGGA T^CCGGA
Bsp1407I 3177 TGTACA T^GTACA
BspEI 2174 TCCGGA T^CCGGA
BsrBI 2390 CCGCTC CCGCTC(-3/-3)
BsrDI 1768 GCAATG GCAATG(2/0)
BsrFI 2954 ACCGGC [GA]^CCGG[CT]
BsrGI 3177 TGTACA T^GTACA
BssSI 2376 CACGAG CACGAG(-5/-1)
BstEII 1952 GGTCACC G^GT[ACGT]ACC
BstPI 1952 GGTCACC G^GT[ACGT]ACC
BstUI 1459 CGCG CG^CG
Cfr10I 2954 ACCGGC [GA]^CCGG[CT]
DrdI 1944 GACACCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3439 AGCGCT AGC^GCT
EcoO65I 1952 GGTCACC G^GT[ACGT]ACC
FbaI 201 TGATCA T^GATCA
HaeII 3439 AGCGCT [GA]GCGC^[CT]
HindIII 2221 AAGCTT A^AGCTT
KpnI 1252 GGTACC GGTAC^C
MfeI 1147 CAATTG C^AATTG
MluI 1458 ACGCGT A^CGCGT
MroI 2174 TCCGGA T^CCGGA
MseI 1381 TTAA T^TAA
MunI 1147 CAATTG C^AATTG
MvnI 1459 CGCG CG^CG
PmaCI 2751 CACGTG CAC^GTG
PmlI 2751 CACGTG CAC^GTG
ScaI 2870 AGTACT AGT^ACT
SphI 3539 GCATGC GCATG^C
SspBI 3177 TGTACA T^GTACA
Tru9I 1381 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 1437 GACGTC GACGT^C
2827 GACGTC
AcsI 459 GAATTT [GA]^AATT[CT]
2180 AAATTC
AhdI 907 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1237 GACGGGAGGTC
ApoI 459 GAATTT [GA]^AATT[CT]
2180 AAATTC
AspEI 907 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1237 GACGGGAGGTC
BbvCI 636 CCTCAGC CCTCAGC(-5/-2)
2517 CCTCAGC
BcgI 2866 CGAGAGTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
370 GCACACGCTTCG
BlpI 1503 GCTGAGC GC^T[ACGT]AGC
2495 GCTCAGC
Bpu1102I 1503 GCTGAGC GC^T[ACGT]AGC
2495 GCTCAGC
BsmI 587 GCATTC GAATGC(1/-1)
975 GCATTC
BstBI 601 TTCGAA TT^CGAA
2737 TTCGAA
CelII 1503 GCTGAGC GC^T[ACGT]AGC
2495 GCTCAGC
CfoI 2027 GCGC GCG^C
3440 GCGC
Eam1105I 907 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1237 GACGGGAGGTC
EcoNI 1848 CCTCCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2688 CCTTCAGAAGG
HhaI 2027 GCGC GCG^C
3440 GCGC
HinP1I 2027 GCGC G^CGC
3440 GCGC
NspV 601 TTCGAA TT^CGAA
2737 TTCGAA
PvuII 2888 CAGCTG CAG^CTG
3382 CAGCTG
SacI 653 GAGCTC GAGCT^C
3522 GAGCTC
SfuI 601 TTCGAA TT^CGAA
2737 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI ApaI Asp700I AspHI
AspI AvaI AvaII BalI BanI
BanII BbvI BciVI BcnI BfaI
BglI BglII BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI