##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccI AccII AclI AflII
AhdI Aor51HI ApaI AscI AseI
AsnI AspEI AspI AviII AvrII
BbeI BcgI BciVI BfrI BglI
BglII BlnI BsaBI BsiEI BsiWI
BspDI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI FseI
FspI HaeII HpaI KasI KspI
MamI MluI MvnI NarI NdeI
NheI NotI NruI PacI PaeR7I
PflFI PflMI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 489 TCCGGA T^CCGGA
AcyI 2209 GGCGTC G[GA]^CG[CT]C
AocI 2481 CCTGAGG CC^T[ACGT]AGG
Asp700I 1700 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 819 GGATCC G^GATCC
BbrPI 238 CACGTG CAC^GTG
BbvCI 2435 CCTCAGC CCTCAGC(-5/-2)
BclI 428 TGATCA T^GATCA
BsaAI 238 CACGTG [CT]AC^GT[GA]
BsaHI 2209 GGCGTC G[GA]^CG[CT]C
BsaI 1393 GAGACC GGTCTC(1/5)
BseAI 489 TCCGGA T^CCGGA
BsmBI 2166 GAGACG CGTCTC(1/5)
BsmI 266 GCATTC GAATGC(1/-1)
Bsp1407I 2157 TGTACA T^GTACA
BspEI 489 TCCGGA T^CCGGA
BspHI 1578 TCATGA T^CATGA
BspLU11I 252 ACATGT A^CATGT
BsrBI 227 GAGCGG CCGCTC(-3/-3)
BsrDI 983 CATTGC GCAATG(2/0)
BsrGI 2157 TGTACA T^GTACA
BstBI 84 TTCGAA TT^CGAA
Bsu36I 2481 CCTGAGG CC^T[ACGT]AGG
CfoI 93 GCGC GCG^C
DraIII 445 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1609 GACAGTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2481 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1262 ATGCAT ATGCA^T
FbaI 428 TGATCA T^GATCA
HhaI 93 GCGC GCG^C
Hin1I 2209 GGCGTC G[GA]^CG[CT]C
HinP1I 93 GCGC G^CGC
HindIII 1294 AAGCTT A^AGCTT
MfeI 785 CAATTG C^AATTG
MroI 489 TCCGGA T^CCGGA
MunI 785 CAATTG C^AATTG
NaeI 1940 GCCGGC GCC^GGC
NgoMIV 1940 GCCGGC G^CCGGC
NsiI 1262 ATGCAT ATGCA^T
NspV 84 TTCGAA TT^CGAA
PmaCI 238 CACGTG CAC^GTG
PmlI 238 CACGTG CAC^GTG
RcaI 1578 TCATGA T^CATGA
SfuI 84 TTCGAA TT^CGAA
SgrAI 1666 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1795 CCCGGG CCC^GGG
SspBI 2157 TGTACA T^GTACA
XcmI 322 CCAGACACTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1795 CCCGGG C^CCGGG
XmaI 1795 CCCGGG C^CCGGG
XmnI 1700 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 220 GGTACC G^GTACC
2405 GGTACC
AgeI 749 ACCGGT A^CCGGT
1667 ACCGGT
Alw44I 459 GTGCAC G^TGCAC
2708 GTGCAC
AlwNI 364 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
560 CAGCCCCTG
ApaLI 459 GTGCAC G^TGCAC
2708 GTGCAC
Asp718I 220 GGTACC G^GTACC
2405 GGTACC
AvaI 1271 CTCGGG C^[CT]CG[GA]G
1795 CCCGGG
BanII 1983 GAGCCC G[GA]GC[CT]^C
2340 GGGCTC
BbsI 397 GAAGAC GAAGAC(2/6)
1828 GAAGAC
BlpI 128 GCTCAGC GC^T[ACGT]AGC
2342 GCTCAGC
Bpu1102I 128 GCTCAGC GC^T[ACGT]AGC
2342 GCTCAGC
BpuAI 397 GAAGAC GAAGAC(2/6)
1828 GAAGAC
BsgI 442 CTGCAC GTGCAG(16/14)
2782 GTGCAG
BsoBI 1271 CTCGGG C^[CT]CG[GA]G
1795 CCCGGG
BstXI 784 CCAATTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1132 CCAGTACAGTGG
CelII 128 GCTCAGC GC^T[ACGT]AGC
2342 GCTCAGC
EcoRV 1408 GATATC GAT^ATC
1435 GATATC
FauI 2812 CCCGC CCCGC(4/6)
2242 GCGGG
HgaI 1224 GCGTC GACGC(5/10)
2210 GCGTC
HincII 1159 GTCAAC GT[CT]^[GA]AC
1660 GTCAAC
HindII 1159 GTCAAC GT[CT]^[GA]AC
1660 GTCAAC
HphI 2670 GGTGA GGTGA(8/7)
350 TCACC
KpnI 220 GGTACC GGTAC^C
2405 GGTACC
MaeII 239 ACGT A^CGT
425 ACGT
MslI 57 CATCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1737 CATGTGTGTG
NcoI 1430 CCATGG C^CATGG
2409 CCATGG
NspI 252 ACATGT [GA]CATG^[CT]
1736 GCATGT
PinAI 749 ACCGGT A^CCGGT
1667 ACCGGT
PstI 640 CTGCAG CTGCA^G
896 CTGCAG
SmlI 1861 CTCAAG C^T[CT][GA]AG
2644 CTTGAG
StuI 48 AGGCCT AGG^CCT
484 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BalI
BanI BbvI BcnI BfaI BmyI
BpmI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII