##################################
# NO CUT ENZYMES ( 189 enzymes) #
##################################
AatII Acc65I AccII AciI AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BglII BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseRI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DpnII DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MamI MboI MfeI
MflI MluI MluNI MscI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI Sau3AI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI TspRI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 160 GTAGAC GT^[AC][GT]AC
AccIII 95 TCCGGA T^CCGGA
AfaI 141 GTAC GT^AC
AlwNI 274 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvrII 290 CCTAGG C^CTAGG
BciVI 266 GTATCC GTATCC(6/5)
BfaI 291 CTAG C^TAG
BlnI 290 CCTAGG C^CTAGG
BsaWI 95 TCCGGA [AT]^CCGG[AT]
BseAI 95 TCCGGA T^CCGGA
BsgI 261 GTGCAG GTGCAG(16/14)
BsiYI 222 CCCTGGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 222 CCCTGGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspEI 95 TCCGGA T^CCGGA
BspMI 211 ACCTGC ACCTGC(4/8)
BsrI 330 CCAGT ACTGG(1/-1)
BstBI 14 TTCGAA TT^CGAA
BstF5I 306 GGATG GGATG(2/0)
BstXI 216 CCATGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EarI 22 GAAGAG CTCTTC(1/4)
EcoT14I 290 CCTAGG C^C[AT][AT]GG
FokI 306 GGATG GGATG(9/13)
HapII 96 CCGG C^CGG
HinfI 85 GACTC G^A[ACGT]TC
HpaII 96 CCGG C^CGG
Ksp632I 22 GAAGAG CTCTTC(1/4)
MaeI 291 CTAG C^TAG
MaeII 353 ACGT A^CGT
MaeIII 44 GTGAC ^GT[ACGT]AC
MnlI 304 GAGG CCTC(7/6)
MroI 95 TCCGGA T^CCGGA
MseI 197 TTAA T^TAA
MslI 150 CACTCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 96 CCGG C^CGG
MwoI 171 GCTGCTTCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspV 14 TTCGAA TT^CGAA
PleI 85 GACTC GAGTC(4/5)
RsaI 141 GTAC GT^AC
SapI 22 GAAGAGC GCTCTTC(1/4)
SfuI 14 TTCGAA TT^CGAA
StyI 290 CCTAGG C^C[AT][AT]GG
TaqI 15 TCGA T^CGA
Tru9I 197 TTAA T^TAA
Tsp45I 44 GTGAC ^GT[GC]AC
Tsp509I 251 AATT ^AATT
TthHB8I 15 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 52 GGACC G^G[AT]CC
256 GGACC
BbsI 35 GTCTTC GAAGAC(2/6)
185 GTCTTC
BpuAI 35 GTCTTC GAAGAC(2/6)
185 GTCTTC
BsmFI 51 GGGAC GGGAC(10/14)
83 GGGAC
BssKI 48 CCTGG ^CC[ACGT]GG
223 CCTGG
BstNI 48 CCTGG CC^[AT]GG
223 CCTGG
Cac8I 27 GCGAGC GC[ACGT]^[ACGT]GC
135 GCAGGC
DdeI 40 CTTAG C^T[ACGT]AG
181 CTAAG
EcoRII 48 CCTGG ^CC[AT]GG
223 CCTGG
HaeIII 207 GGCC GG^CC
310 GGCC
MvaI 48 CCTGG CC^[AT]GG
223 CCTGG
NlaIII 56 CATG CATG^
217 CATG
NlaIV 51 GGGACC GG[ACGT]^[ACGT]CC
310 GGCCCC
ScrFI 48 CCTGG CC^[ACGT]GG
223 CCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AluI BbvI BsaJI Cfr13I Fnu4HI
ItaI MboII Sau96I TseI