################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BcgI BglI BglII BlnI BsaHI BsaWI BseAI BsiWI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BstBI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I EcoRI EcoRV FauI FseI FspI HgaI Hin1I KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 1371 CGCG CG^CG AcsI 708 AAATTC [GA]^AATT[CT] AflII 328 CTTAAG C^TTAAG AocI 602 CCTGAGG CC^T[ACGT]AGG Aor51HI 859 AGCGCT AGC^GCT ApaI 1981 GGGCCC GGGCC^C ApoI 708 AAATTC [GA]^AATT[CT] AvaI 2517 CTCGGG C^[CT]CG[GA]G BamHI 1214 GGATCC G^GATCC BbrPI 194 CACGTG CAC^GTG BbvCI 1145 CCTCAGC CCTCAGC(-5/-2) BciVI 2309 GTATCC GTATCC(6/5) BclI 2355 TGATCA T^GATCA BfrI 328 CTTAAG C^TTAAG BsaBI 287 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1924 GAGACC GGTCTC(1/5) BsiEI 132 CGGTCG CG[GA][CT]^CG BsmBI 128 GAGACG CGTCTC(1/5) BsoBI 2517 CTCGGG C^[CT]CG[GA]G BspHI 699 TCATGA T^CATGA BsrDI 2504 GCAATG GCAATG(2/0) BssSI 1855 CACGAG CACGAG(-5/-1) Bst1107I 98 GTATAC GTA^TAC BstEII 203 GGTCACC G^GT[ACGT]ACC BstPI 203 GGTCACC G^GT[ACGT]ACC BstUI 1371 CGCG CG^CG BstZ17I 98 GTATAC GTA^TAC Bsu36I 602 CCTGAGG CC^T[ACGT]AGG DraI 2651 TTTAAA TTT^AAA Eco47III 859 AGCGCT AGC^GCT Eco81I 602 CCTGAGG CC^T[ACGT]AGG EcoO65I 203 GGTCACC G^GT[ACGT]ACC FbaI 2355 TGATCA T^GATCA HaeII 859 AGCGCT [GA]GCGC^[CT] HpaI 2405 GTTAAC GTT^AAC MamI 287 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 1371 CGCG CG^CG PflMI 475 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 165 GACTC GAGTC(4/5) PmaCI 194 CACGTG CAC^GTG PmlI 194 CACGTG CAC^GTG RcaI 699 TCATGA T^CATGA SalI 2036 GTCGAC G^TCGAC SpeI 994 ACTAGT A^CTAGT StuI 1760 AGGCCT AGG^CCT TfiI 2775 GATTC G^A[AT]TC Van91I 475 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2344 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwNI 114 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG 2077 CAGATACTG Asp700I 629 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1667 GAAGGATTTC BanI 1012 GGCACC G^G[CT][GA]CC 1384 GGCACC BbsI 622 GAAGAC GAAGAC(2/6) 2795 GTCTTC BcnI 1515 CCCGG CC^[GC]GG 1606 CCGGG BlpI 554 GCTCAGC GC^T[ACGT]AGC 608 GCTCAGC Bpu1102I 554 GCTCAGC GC^T[ACGT]AGC 608 GCTCAGC BpuAI 622 GAAGAC GAAGAC(2/6) 2795 GTCTTC BsaAI 194 CACGTG [CT]AC^GT[GA] 2327 CACGTA BseRI 1173 GAGGAG GAGGAG(10/8) 2729 GAGGAG BsmI 1349 GCATTC GAATGC(1/-1) 2043 GCATTC BstAPI 113 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 253 GCATAAAATGC CelII 554 GCTCAGC GC^T[ACGT]AGC 608 GCTCAGC CfoI 262 GCGC GCG^C 860 GCGC EcoNI 1130 CCTGTAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1932 CCTTTGGAAGG EcoT22I 106 ATGCAT ATGCA^T 2493 ATGCAT HhaI 262 GCGC GCG^C 860 GCGC HinP1I 262 GCGC G^CGC 860 GCGC HincII 2036 GTCGAC GT[CT]^[GA]AC 2405 GTTAAC HindII 2036 GTCGAC GT[CT]^[GA]AC 2405 GTTAAC HindIII 2245 AAGCTT A^AGCTT 2947 AAGCTT HinfI 165 GACTC G^A[ACGT]TC 2775 GATTC NciI 1515 CCCGG CC^[GC]GG 1606 CCGGG NsiI 106 ATGCAT ATGCA^T 2493 ATGCAT PpuMI 688 AGGACCT [GA]G^G[AT]CC[CT] 2520 GGGACCT XmnI 629 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1667 GAAGGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccI AciI AfaI AluI AlwI AspHI AvaII BalI BanII BbvI BfaI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII