##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BbeI BcgI
BglI BglII BlnI BsaHI BsaWI
BseAI BsiWI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BstBI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV FauI FseI FspI HgaI
Hin1I KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspI SwaI Tth111I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 1371 CGCG CG^CG
AcsI 708 AAATTC [GA]^AATT[CT]
AflII 328 CTTAAG C^TTAAG
AocI 602 CCTGAGG CC^T[ACGT]AGG
Aor51HI 859 AGCGCT AGC^GCT
ApaI 1981 GGGCCC GGGCC^C
ApoI 708 AAATTC [GA]^AATT[CT]
AvaI 2517 CTCGGG C^[CT]CG[GA]G
BamHI 1214 GGATCC G^GATCC
BbrPI 194 CACGTG CAC^GTG
BbvCI 1145 CCTCAGC CCTCAGC(-5/-2)
BciVI 2309 GTATCC GTATCC(6/5)
BclI 2355 TGATCA T^GATCA
BfrI 328 CTTAAG C^TTAAG
BsaBI 287 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1924 GAGACC GGTCTC(1/5)
BsiEI 132 CGGTCG CG[GA][CT]^CG
BsmBI 128 GAGACG CGTCTC(1/5)
BsoBI 2517 CTCGGG C^[CT]CG[GA]G
BspHI 699 TCATGA T^CATGA
BsrDI 2504 GCAATG GCAATG(2/0)
BssSI 1855 CACGAG CACGAG(-5/-1)
Bst1107I 98 GTATAC GTA^TAC
BstEII 203 GGTCACC G^GT[ACGT]ACC
BstPI 203 GGTCACC G^GT[ACGT]ACC
BstUI 1371 CGCG CG^CG
BstZ17I 98 GTATAC GTA^TAC
Bsu36I 602 CCTGAGG CC^T[ACGT]AGG
DraI 2651 TTTAAA TTT^AAA
Eco47III 859 AGCGCT AGC^GCT
Eco81I 602 CCTGAGG CC^T[ACGT]AGG
EcoO65I 203 GGTCACC G^GT[ACGT]ACC
FbaI 2355 TGATCA T^GATCA
HaeII 859 AGCGCT [GA]GCGC^[CT]
HpaI 2405 GTTAAC GTT^AAC
MamI 287 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1371 CGCG CG^CG
PflMI 475 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 165 GACTC GAGTC(4/5)
PmaCI 194 CACGTG CAC^GTG
PmlI 194 CACGTG CAC^GTG
RcaI 699 TCATGA T^CATGA
SalI 2036 GTCGAC G^TCGAC
SpeI 994 ACTAGT A^CTAGT
StuI 1760 AGGCCT AGG^CCT
TfiI 2775 GATTC G^A[AT]TC
Van91I 475 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2344 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 114 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2077 CAGATACTG
Asp700I 629 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1667 GAAGGATTTC
BanI 1012 GGCACC G^G[CT][GA]CC
1384 GGCACC
BbsI 622 GAAGAC GAAGAC(2/6)
2795 GTCTTC
BcnI 1515 CCCGG CC^[GC]GG
1606 CCGGG
BlpI 554 GCTCAGC GC^T[ACGT]AGC
608 GCTCAGC
Bpu1102I 554 GCTCAGC GC^T[ACGT]AGC
608 GCTCAGC
BpuAI 622 GAAGAC GAAGAC(2/6)
2795 GTCTTC
BsaAI 194 CACGTG [CT]AC^GT[GA]
2327 CACGTA
BseRI 1173 GAGGAG GAGGAG(10/8)
2729 GAGGAG
BsmI 1349 GCATTC GAATGC(1/-1)
2043 GCATTC
BstAPI 113 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
253 GCATAAAATGC
CelII 554 GCTCAGC GC^T[ACGT]AGC
608 GCTCAGC
CfoI 262 GCGC GCG^C
860 GCGC
EcoNI 1130 CCTGTAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1932 CCTTTGGAAGG
EcoT22I 106 ATGCAT ATGCA^T
2493 ATGCAT
HhaI 262 GCGC GCG^C
860 GCGC
HinP1I 262 GCGC G^CGC
860 GCGC
HincII 2036 GTCGAC GT[CT]^[GA]AC
2405 GTTAAC
HindII 2036 GTCGAC GT[CT]^[GA]AC
2405 GTTAAC
HindIII 2245 AAGCTT A^AGCTT
2947 AAGCTT
HinfI 165 GACTC G^A[ACGT]TC
2775 GATTC
NciI 1515 CCCGG CC^[GC]GG
1606 CCGGG
NsiI 106 ATGCAT ATGCA^T
2493 ATGCAT
PpuMI 688 AGGACCT [GA]G^G[AT]CC[CT]
2520 GGGACCT
XmnI 629 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1667 GAAGGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AspHI AvaII BalI BanII BbvI
BfaI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII