##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AgeI Alw44I ApaI ApaLI
AscI AseI AsnI AspI AviII
AvrII BamHI BbeI BclI BfrI
BglI BglII BlnI BsaBI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I ClaI
DraI DraIII DrdI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI MvnI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 184 GGTACC G^GTACC
AcsI 889 GAATTC [GA]^AATT[CT]
AcyI 2077 GACGCC G[GA]^CG[CT]C
AhdI 1401 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 499 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2051 AGCGCT AGC^GCT
ApoI 889 GAATTC [GA]^AATT[CT]
Asp700I 885 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 184 GGTACC G^GTACC
AspEI 1401 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1411 CACGTG CAC^GTG
BbvCI 597 GCTGAGG CCTCAGC(-5/-2)
BcgI 1617 CGAAGATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 755 GTATCC GTATCC(6/5)
BsaAI 1411 CACGTG [CT]AC^GT[GA]
BsaHI 2077 GACGCC G[GA]^CG[CT]C
BsaWI 368 TCCGGT [AT]^CCGG[AT]
BsiEI 1242 CGGCCG CG[GA][CT]^CG
BsmI 651 GCATTC GAATGC(1/-1)
BspMI 335 ACCTGC ACCTGC(4/8)
BsrDI 1812 CATTGC GCAATG(2/0)
BsrFI 983 GCCGGC [GA]^CCGG[CT]
BstAPI 1807 GCACCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 450 GGTGACC G^GT[ACGT]ACC
BstPI 450 GGTGACC G^GT[ACGT]ACC
BstXI 2091 CCACATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 499 CCTAAGG CC^T[ACGT]AGG
CfoI 2052 GCGC GCG^C
Cfr10I 983 GCCGGC [GA]^CCGG[CT]
CpoI 932 CGGTCCG CG^G[AT]CCG
EagI 1242 CGGCCG C^GGCCG
Eam1105I 1401 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1242 CGGCCG C^GGCCG
Eco47III 2051 AGCGCT AGC^GCT
Eco52I 1242 CGGCCG C^GGCCG
Eco81I 499 CCTAAGG CC^T[ACGT]AGG
EcoO65I 450 GGTGACC G^GT[ACGT]ACC
EcoRI 889 GAATTC G^AATTC
HaeII 2051 AGCGCT [GA]GCGC^[CT]
HgaI 2077 GACGC GACGC(5/10)
HhaI 2052 GCGC GCG^C
Hin1I 2077 GACGCC G[GA]^CG[CT]C
HinP1I 2052 GCGC G^CGC
KpnI 184 GGTACC GGTAC^C
NaeI 983 GCCGGC GCC^GGC
NgoMIV 983 GCCGGC G^CCGGC
NspI 1706 ACATGC [GA]CATG^[CT]
PmaCI 1411 CACGTG CAC^GTG
PmlI 1411 CACGTG CAC^GTG
RsrII 932 CGGTCCG CG^G[AT]CCG
SapI 991 GAAGAGC GCTCTTC(1/4)
SmaI 101 CCCGGG CCC^GGG
TaqI 243 TCGA T^CGA
TfiI 1444 GATTC G^A[AT]TC
TthHB8I 243 TCGA T^CGA
XmaCI 101 CCCGGG C^CCGGG
XmaI 101 CCCGGG C^CCGGG
XmnI 885 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 254 ACACGT A^C[GA][CT]GT
1412 ACGTGT
BanI 93 GGCACC G^G[CT][GA]CC
184 GGTACC
BbsI 1380 GAAGAC GAAGAC(2/6)
1702 GAAGAC
BpuAI 1380 GAAGAC GAAGAC(2/6)
1702 GAAGAC
BsaI 740 GAGACC GGTCTC(1/5)
1187 GAGACC
BsrBI 544 GAGCGG CCGCTC(-3/-3)
1300 GAGCGG
BstYI 1343 AGATCC [GA]^GATC[CT]
1364 AGATCC
HincII 112 GTCAAC GT[CT]^[GA]AC
1237 GTCAAC
HindII 112 GTCAAC GT[CT]^[GA]AC
1237 GTCAAC
MflI 1343 AGATCC [GA]^GATC[CT]
1364 AGATCC
MseI 221 TTAA T^TAA
955 TTAA
PflMI 200 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1717 CCATCCTATGG
SmlI 58 CTTGAG C^T[CT][GA]AG
1128 CTCAAG
Tru9I 221 TTAA T^TAA
955 TTAA
Van91I 200 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1717 CCATCCTATGG
XcmI 146 CCAACACCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
437 CCATGGATGAGCTGG
XhoII 1343 AGATCC [GA]^GATC[CT]
1364 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanII
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CelII Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I Tsp509I TspRI