################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AgeI Alw44I ApaI ApaLI AscI AseI AsnI AspI AviII AvrII BamHI BbeI BclI BfrI BglI BglII BlnI BsaBI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I ClaI DraI DraIII DrdI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KspI MamI MfeI MluI MroI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 184 GGTACC G^GTACC AcsI 889 GAATTC [GA]^AATT[CT] AcyI 2077 GACGCC G[GA]^CG[CT]C AhdI 1401 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 499 CCTAAGG CC^T[ACGT]AGG Aor51HI 2051 AGCGCT AGC^GCT ApoI 889 GAATTC [GA]^AATT[CT] Asp700I 885 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 184 GGTACC G^GTACC AspEI 1401 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 1411 CACGTG CAC^GTG BbvCI 597 GCTGAGG CCTCAGC(-5/-2) BcgI 1617 CGAAGATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 755 GTATCC GTATCC(6/5) BsaAI 1411 CACGTG [CT]AC^GT[GA] BsaHI 2077 GACGCC G[GA]^CG[CT]C BsaWI 368 TCCGGT [AT]^CCGG[AT] BsiEI 1242 CGGCCG CG[GA][CT]^CG BsmI 651 GCATTC GAATGC(1/-1) BspMI 335 ACCTGC ACCTGC(4/8) BsrDI 1812 CATTGC GCAATG(2/0) BsrFI 983 GCCGGC [GA]^CCGG[CT] BstAPI 1807 GCACCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 450 GGTGACC G^GT[ACGT]ACC BstPI 450 GGTGACC G^GT[ACGT]ACC BstXI 2091 CCACATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 499 CCTAAGG CC^T[ACGT]AGG CfoI 2052 GCGC GCG^C Cfr10I 983 GCCGGC [GA]^CCGG[CT] CpoI 932 CGGTCCG CG^G[AT]CCG EagI 1242 CGGCCG C^GGCCG Eam1105I 1401 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1242 CGGCCG C^GGCCG Eco47III 2051 AGCGCT AGC^GCT Eco52I 1242 CGGCCG C^GGCCG Eco81I 499 CCTAAGG CC^T[ACGT]AGG EcoO65I 450 GGTGACC G^GT[ACGT]ACC EcoRI 889 GAATTC G^AATTC HaeII 2051 AGCGCT [GA]GCGC^[CT] HgaI 2077 GACGC GACGC(5/10) HhaI 2052 GCGC GCG^C Hin1I 2077 GACGCC G[GA]^CG[CT]C HinP1I 2052 GCGC G^CGC KpnI 184 GGTACC GGTAC^C NaeI 983 GCCGGC GCC^GGC NgoMIV 983 GCCGGC G^CCGGC NspI 1706 ACATGC [GA]CATG^[CT] PmaCI 1411 CACGTG CAC^GTG PmlI 1411 CACGTG CAC^GTG RsrII 932 CGGTCCG CG^G[AT]CCG SapI 991 GAAGAGC GCTCTTC(1/4) SmaI 101 CCCGGG CCC^GGG TaqI 243 TCGA T^CGA TfiI 1444 GATTC G^A[AT]TC TthHB8I 243 TCGA T^CGA XmaCI 101 CCCGGG C^CCGGG XmaI 101 CCCGGG C^CCGGG XmnI 885 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AflIII 254 ACACGT A^C[GA][CT]GT 1412 ACGTGT BanI 93 GGCACC G^G[CT][GA]CC 184 GGTACC BbsI 1380 GAAGAC GAAGAC(2/6) 1702 GAAGAC BpuAI 1380 GAAGAC GAAGAC(2/6) 1702 GAAGAC BsaI 740 GAGACC GGTCTC(1/5) 1187 GAGACC BsrBI 544 GAGCGG CCGCTC(-3/-3) 1300 GAGCGG BstYI 1343 AGATCC [GA]^GATC[CT] 1364 AGATCC HincII 112 GTCAAC GT[CT]^[GA]AC 1237 GTCAAC HindII 112 GTCAAC GT[CT]^[GA]AC 1237 GTCAAC MflI 1343 AGATCC [GA]^GATC[CT] 1364 AGATCC MseI 221 TTAA T^TAA 955 TTAA PflMI 200 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1717 CCATCCTATGG SmlI 58 CTTGAG C^T[CT][GA]AG 1128 CTCAAG Tru9I 221 TTAA T^TAA 955 TTAA Van91I 200 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1717 CCATCCTATGG XcmI 146 CCAACACCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 437 CCATGGATGAGCTGG XhoII 1343 AGATCC [GA]^GATC[CT] 1364 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CelII Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I Tsp509I TspRI