##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AflII       AgeI        Alw44I      ApaI        ApaLI       
AscI        AseI        AsnI        AspI        AviII       
AvrII       BamHI       BbeI        BclI        BfrI        
BglI        BglII       BlnI        BsaBI       BseAI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstUI       BstZ17I     ClaI        
DraI        DraIII      DrdI        EcoRV       EcoT22I     
FbaI        FseI        FspI        HindIII     HpaI        
KasI        KspI        MamI        MfeI        MluI        
MroI        MunI        MvnI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

Acc65I        184  GGTACC               G^GTACC
AcsI          889  GAATTC               [GA]^AATT[CT]
AcyI         2077  GACGCC               G[GA]^CG[CT]C
AhdI         1401  GACCCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          499  CCTAAGG              CC^T[ACGT]AGG
Aor51HI      2051  AGCGCT               AGC^GCT
ApoI          889  GAATTC               [GA]^AATT[CT]
Asp700I       885  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       184  GGTACC               G^GTACC
AspEI        1401  GACCCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI        1411  CACGTG               CAC^GTG
BbvCI         597  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1617  CGAAGATGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         755  GTATCC               GTATCC(6/5)
BsaAI        1411  CACGTG               [CT]AC^GT[GA]
BsaHI        2077  GACGCC               G[GA]^CG[CT]C
BsaWI         368  TCCGGT               [AT]^CCGG[AT]
BsiEI        1242  CGGCCG               CG[GA][CT]^CG
BsmI          651  GCATTC               GAATGC(1/-1)
BspMI         335  ACCTGC               ACCTGC(4/8)
BsrDI        1812  CATTGC               GCAATG(2/0)
BsrFI         983  GCCGGC               [GA]^CCGG[CT]
BstAPI       1807  GCACCCATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        450  GGTGACC              G^GT[ACGT]ACC
BstPI         450  GGTGACC              G^GT[ACGT]ACC
BstXI        2091  CCACATGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        499  CCTAAGG              CC^T[ACGT]AGG
CfoI         2052  GCGC                 GCG^C
Cfr10I        983  GCCGGC               [GA]^CCGG[CT]
CpoI          932  CGGTCCG              CG^G[AT]CCG
EagI         1242  CGGCCG               C^GGCCG
Eam1105I     1401  GACCCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1242  CGGCCG               C^GGCCG
Eco47III     2051  AGCGCT               AGC^GCT
Eco52I       1242  CGGCCG               C^GGCCG
Eco81I        499  CCTAAGG              CC^T[ACGT]AGG
EcoO65I       450  GGTGACC              G^GT[ACGT]ACC
EcoRI         889  GAATTC               G^AATTC
HaeII        2051  AGCGCT               [GA]GCGC^[CT]
HgaI         2077  GACGC                GACGC(5/10)
HhaI         2052  GCGC                 GCG^C
Hin1I        2077  GACGCC               G[GA]^CG[CT]C
HinP1I       2052  GCGC                 G^CGC
KpnI          184  GGTACC               GGTAC^C
NaeI          983  GCCGGC               GCC^GGC
NgoMIV        983  GCCGGC               G^CCGGC
NspI         1706  ACATGC               [GA]CATG^[CT]
PmaCI        1411  CACGTG               CAC^GTG
PmlI         1411  CACGTG               CAC^GTG
RsrII         932  CGGTCCG              CG^G[AT]CCG
SapI          991  GAAGAGC              GCTCTTC(1/4)
SmaI          101  CCCGGG               CCC^GGG
TaqI          243  TCGA                 T^CGA
TfiI         1444  GATTC                G^A[AT]TC
TthHB8I       243  TCGA                 T^CGA
XmaCI         101  CCCGGG               C^CCGGG
XmaI          101  CCCGGG               C^CCGGG
XmnI          885  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AflIII        254  ACACGT               A^C[GA][CT]GT
             1412  ACGTGT              
BanI           93  GGCACC               G^G[CT][GA]CC
              184  GGTACC              
BbsI         1380  GAAGAC               GAAGAC(2/6)
             1702  GAAGAC              
BpuAI        1380  GAAGAC               GAAGAC(2/6)
             1702  GAAGAC              
BsaI          740  GAGACC               GGTCTC(1/5)
             1187  GAGACC              
BsrBI         544  GAGCGG               CCGCTC(-3/-3)
             1300  GAGCGG              
BstYI        1343  AGATCC               [GA]^GATC[CT]
             1364  AGATCC              
HincII        112  GTCAAC               GT[CT]^[GA]AC
             1237  GTCAAC              
HindII        112  GTCAAC               GT[CT]^[GA]AC
             1237  GTCAAC              
MflI         1343  AGATCC               [GA]^GATC[CT]
             1364  AGATCC              
MseI          221  TTAA                 T^TAA
              955  TTAA                
PflMI         200  CCAGTGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1717  CCATCCTATGG         
SmlI           58  CTTGAG               C^T[CT][GA]AG
             1128  CTCAAG              
Tru9I         221  TTAA                 T^TAA
              955  TTAA                
Van91I        200  CCAGTGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1717  CCATCCTATGG         
XcmI          146  CCAACACCAAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              437  CCATGGATGAGCTGG     
XhoII        1343  AGATCC               [GA]^GATC[CT]
             1364  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaI        AvaII       BalI        BanII       
BbvI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CelII       Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TseI        
Tsp45I      Tsp509I     TspRI