################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AhdI Alw44I AlwI AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvaI AviII BamHI BbeI BcgI BciVI BcnI BfrI BglI BsaBI BsaHI BseAI BsiEI BsiWI BsoBI BspDI BspEI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoT22I FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI RsrII SacI SacII ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AgeI 1096 ACCGGT A^CCGGT ApaI 1685 GGGCCC GGGCC^C Asp700I 1004 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 356 GAGCAC G[AT]GC[AT]^C AvrII 437 CCTAGG C^CTAGG BanI 139 GGCACC G^G[CT][GA]CC BclI 1282 TGATCA T^GATCA BglII 1006 AGATCT A^GATCT BlnI 437 CCTAGG C^CTAGG BlpI 302 GCTAAGC GC^T[ACGT]AGC Bpu1102I 302 GCTAAGC GC^T[ACGT]AGC BsaI 385 GAGACC GGTCTC(1/5) BsiHKAI 356 GAGCAC G[AT]GC[AT]^C BsmBI 983 GAGACG CGTCTC(1/5) Bsp1407I 481 TGTACA T^GTACA BspHI 1012 TCATGA T^CATGA BspLU11I 919 ACATGT A^CATGT BsrBI 45 CCGCTC CCGCTC(-3/-3) BsrGI 481 TGTACA T^GTACA BssSI 1036 CTCGTG CACGAG(-5/-1) BstEII 494 GGTGACC G^GT[ACGT]ACC BstPI 494 GGTGACC G^GT[ACGT]ACC BstYI 1006 AGATCT [GA]^GATC[CT] CelII 302 GCTAAGC GC^T[ACGT]AGC CfoI 89 GCGC GCG^C DraIII 960 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 494 GGTGACC G^GT[ACGT]ACC FbaI 1282 TGATCA T^GATCA HaeII 88 AGCGCC [GA]GCGC^[CT] HhaI 89 GCGC GCG^C HinP1I 89 GCGC G^CGC MflI 1006 AGATCT [GA]^GATC[CT] NcoI 1501 CCATGG C^CATGG PinAI 1096 ACCGGT A^CCGGT RcaI 1012 TCATGA T^CATGA SapI 1847 GCTCTTC GCTCTTC(1/4) SbfI 566 CCTGCAGG CCTGCA^GG SmlI 1990 CTCAAG C^T[CT][GA]AG SphI 1489 GCATGC GCATG^C Sse8387I 566 CCTGCAGG CCTGCA^GG SspBI 481 TGTACA T^GTACA Tsp509I 1347 AATT ^AATT XhoII 1006 AGATCT [GA]^GATC[CT] XmnI 1004 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccI 1 GTCGAC GT^[AC][GT]AC 1143 GTCGAC AflIII 919 ACATGT A^C[GA][CT]GT 1642 ACGTGT AspI 321 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1587 GACAGTGTC BalI 739 TGGCCA TGG^CCA 1301 TGGCCA BbrPI 1641 CACGTG CAC^GTG 1698 CACGTG BsaWI 786 ACCGGA [AT]^CCGG[AT] 1096 ACCGGT BseRI 839 CTCCTC GAGGAG(10/8) 1977 CTCCTC BsmFI 1262 GTCCC GGGAC(10/14) 1646 GTCCC BsmI 1776 GCATTC GAATGC(1/-1) 1784 GCATTC BsrDI 803 CATTGC GCAATG(2/0) 133 GCAATG BsrFI 1096 ACCGGT [GA]^CCGG[CT] 1566 ACCGGC Cfr10I 1096 ACCGGT [GA]^CCGG[CT] 1566 ACCGGC DpnII 1007 GATC ^GATC 1283 GATC DrdI 969 GACTGTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1416 GACCCTGCTGTC DsaI 346 CCGTGG C^C[GA][CT]GG 1501 CCATGG EarI 846 CTCTTC CTCTTC(1/4) 1848 CTCTTC EcoRV 834 GATATC GAT^ATC 1623 GATATC Ksp632I 846 CTCTTC CTCTTC(1/4) 1848 CTCTTC MboI 1007 GATC ^GATC 1283 GATC MluNI 739 TGGCCA TGG^CCA 1301 TGGCCA MscI 739 TGGCCA TGG^CCA 1301 TGGCCA MseI 112 TTAA T^TAA 1893 TTAA NdeII 1007 GATC ^GATC 1283 GATC PflFI 321 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1587 GACAGTGTC PflMI 527 CCACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 959 CCACCCTGTGG PmaCI 1641 CACGTG CAC^GTG 1698 CACGTG PmlI 1641 CACGTG CAC^GTG 1698 CACGTG PshAI 497 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 557 GACCCACGTC PstI 567 CTGCAG CTGCA^G 1078 CTGCAG PvuII 546 CAGCTG CAG^CTG 1575 CAGCTG SalI 1 GTCGAC G^TCGAC 1143 GTCGAC Sau3AI 1007 GATC ^GATC 1283 GATC StuI 440 AGGCCT AGG^CCT 902 AGGCCT TfiI 296 GATTC G^A[AT]TC 676 GAATC Tru9I 112 TTAA T^TAA 1893 TTAA Tth111I 321 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1587 GACAGTGTC Van91I 527 CCACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 959 CCACCCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AfaI AluI AocI AvaII BanII BbsI BbvCI BbvI BfaI BmyI BpmI BpuAI BsaAI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI Bsu36I Cac8I Cfr13I DdeI DraII EaeI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboII MnlI MslI MspA1I MspI MvaI MwoI NlaIII NlaIV NspI PleI PpuMI RsaI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I XcmI