##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AhdI Alw44I
AlwI AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AvaI AviII BamHI BbeI BcgI
BciVI BcnI BfrI BglI BsaBI
BsaHI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoT22I FseI
FspI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshBI Psp1406I PvuI RsrII
SacI SacII ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspI SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AgeI 1096 ACCGGT A^CCGGT
ApaI 1685 GGGCCC GGGCC^C
Asp700I 1004 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 356 GAGCAC G[AT]GC[AT]^C
AvrII 437 CCTAGG C^CTAGG
BanI 139 GGCACC G^G[CT][GA]CC
BclI 1282 TGATCA T^GATCA
BglII 1006 AGATCT A^GATCT
BlnI 437 CCTAGG C^CTAGG
BlpI 302 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 302 GCTAAGC GC^T[ACGT]AGC
BsaI 385 GAGACC GGTCTC(1/5)
BsiHKAI 356 GAGCAC G[AT]GC[AT]^C
BsmBI 983 GAGACG CGTCTC(1/5)
Bsp1407I 481 TGTACA T^GTACA
BspHI 1012 TCATGA T^CATGA
BspLU11I 919 ACATGT A^CATGT
BsrBI 45 CCGCTC CCGCTC(-3/-3)
BsrGI 481 TGTACA T^GTACA
BssSI 1036 CTCGTG CACGAG(-5/-1)
BstEII 494 GGTGACC G^GT[ACGT]ACC
BstPI 494 GGTGACC G^GT[ACGT]ACC
BstYI 1006 AGATCT [GA]^GATC[CT]
CelII 302 GCTAAGC GC^T[ACGT]AGC
CfoI 89 GCGC GCG^C
DraIII 960 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 494 GGTGACC G^GT[ACGT]ACC
FbaI 1282 TGATCA T^GATCA
HaeII 88 AGCGCC [GA]GCGC^[CT]
HhaI 89 GCGC GCG^C
HinP1I 89 GCGC G^CGC
MflI 1006 AGATCT [GA]^GATC[CT]
NcoI 1501 CCATGG C^CATGG
PinAI 1096 ACCGGT A^CCGGT
RcaI 1012 TCATGA T^CATGA
SapI 1847 GCTCTTC GCTCTTC(1/4)
SbfI 566 CCTGCAGG CCTGCA^GG
SmlI 1990 CTCAAG C^T[CT][GA]AG
SphI 1489 GCATGC GCATG^C
Sse8387I 566 CCTGCAGG CCTGCA^GG
SspBI 481 TGTACA T^GTACA
Tsp509I 1347 AATT ^AATT
XhoII 1006 AGATCT [GA]^GATC[CT]
XmnI 1004 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1 GTCGAC GT^[AC][GT]AC
1143 GTCGAC
AflIII 919 ACATGT A^C[GA][CT]GT
1642 ACGTGT
AspI 321 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1587 GACAGTGTC
BalI 739 TGGCCA TGG^CCA
1301 TGGCCA
BbrPI 1641 CACGTG CAC^GTG
1698 CACGTG
BsaWI 786 ACCGGA [AT]^CCGG[AT]
1096 ACCGGT
BseRI 839 CTCCTC GAGGAG(10/8)
1977 CTCCTC
BsmFI 1262 GTCCC GGGAC(10/14)
1646 GTCCC
BsmI 1776 GCATTC GAATGC(1/-1)
1784 GCATTC
BsrDI 803 CATTGC GCAATG(2/0)
133 GCAATG
BsrFI 1096 ACCGGT [GA]^CCGG[CT]
1566 ACCGGC
Cfr10I 1096 ACCGGT [GA]^CCGG[CT]
1566 ACCGGC
DpnII 1007 GATC ^GATC
1283 GATC
DrdI 969 GACTGTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1416 GACCCTGCTGTC
DsaI 346 CCGTGG C^C[GA][CT]GG
1501 CCATGG
EarI 846 CTCTTC CTCTTC(1/4)
1848 CTCTTC
EcoRV 834 GATATC GAT^ATC
1623 GATATC
Ksp632I 846 CTCTTC CTCTTC(1/4)
1848 CTCTTC
MboI 1007 GATC ^GATC
1283 GATC
MluNI 739 TGGCCA TGG^CCA
1301 TGGCCA
MscI 739 TGGCCA TGG^CCA
1301 TGGCCA
MseI 112 TTAA T^TAA
1893 TTAA
NdeII 1007 GATC ^GATC
1283 GATC
PflFI 321 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1587 GACAGTGTC
PflMI 527 CCACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
959 CCACCCTGTGG
PmaCI 1641 CACGTG CAC^GTG
1698 CACGTG
PmlI 1641 CACGTG CAC^GTG
1698 CACGTG
PshAI 497 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
557 GACCCACGTC
PstI 567 CTGCAG CTGCA^G
1078 CTGCAG
PvuII 546 CAGCTG CAG^CTG
1575 CAGCTG
SalI 1 GTCGAC G^TCGAC
1143 GTCGAC
Sau3AI 1007 GATC ^GATC
1283 GATC
StuI 440 AGGCCT AGG^CCT
902 AGGCCT
TfiI 296 GATTC G^A[AT]TC
676 GAATC
Tru9I 112 TTAA T^TAA
1893 TTAA
Tth111I 321 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1587 GACAGTGTC
Van91I 527 CCACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
959 CCACCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AocI AvaII
BanII BbsI BbvCI BbvI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BsgI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI Bsu36I Cac8I Cfr13I DdeI
DraII EaeI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboII MnlI MslI MspA1I
MspI MvaI MwoI NlaIII NlaIV
NspI PleI PpuMI RsaI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I XcmI