##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AccI AccII AccIII AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BcnI BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HpaI HpaII KasI KspI MamI
MfeI MluI MroI MslI MspI
MunI MvnI NaeI NarI NciI
NgoMIV NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AclI 204 AACGTT AA^CGTT
AlwI 944 GATCC GGATC(4/5)
AvaI 373 CCCGAG C^[CT]CG[GA]G
BalI 1979 TGGCCA TGG^CCA
BbsI 2006 GAAGAC GAAGAC(2/6)
BclI 646 TGATCA T^GATCA
BglI 1453 GCCTGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1052 AGATCT A^GATCT
BpuAI 2006 GAAGAC GAAGAC(2/6)
BsaI 1728 GAGACC GGTCTC(1/5)
BsgI 97 GTGCAG GTGCAG(16/14)
BsoBI 373 CCCGAG C^[CT]CG[GA]G
BspMI 1449 ACCTGC ACCTGC(4/8)
BsrDI 2038 GCAATG GCAATG(2/0)
BssSI 2328 CTCGTG CACGAG(-5/-1)
BstAPI 1266 GCAGTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 363 TTCGAA TT^CGAA
BstYI 1052 AGATCT [GA]^GATC[CT]
DraII 916 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1990 CCATGG C^C[GA][CT]GG
EaeI 1979 TGGCCA [CT]^GGCC[GA]
EcoO109I 916 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 1783 CCCGC CCCGC(4/6)
FbaI 646 TGATCA T^GATCA
HgaI 155 GACGC GACGC(5/10)
HincII 669 GTTGAC GT[CT]^[GA]AC
HindII 669 GTTGAC GT[CT]^[GA]AC
HindIII 277 AAGCTT A^AGCTT
MaeII 205 ACGT A^CGT
MflI 1052 AGATCT [GA]^GATC[CT]
MluNI 1979 TGGCCA TGG^CCA
MscI 1979 TGGCCA TGG^CCA
NcoI 1990 CCATGG C^CATGG
NdeI 1764 CATATG CA^TATG
NspV 363 TTCGAA TT^CGAA
PflMI 104 CCAAAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 916 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 204 AACGTT AA^CGTT
SacI 1867 GAGCTC GAGCT^C
SapI 1786 GCTCTTC GCTCTTC(1/4)
SfuI 363 TTCGAA TT^CGAA
Van91I 104 CCAAAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 749 TCTAGA T^CTAGA
XcmI 2141 CCAATACTGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1052 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Acc65I 213 GGTACC G^GTACC
2212 GGTACC
AflII 674 CTTAAG C^TTAAG
948 CTTAAG
Asp718I 213 GGTACC G^GTACC
2212 GGTACC
AspHI 1437 GAGCAC G[AT]GC[AT]^C
1867 GAGCTC
AvaII 917 GGACC G^G[AT]CC
1333 GGTCC
BanI 213 GGTACC G^G[CT][GA]CC
2212 GGTACC
BfrI 674 CTTAAG C^TTAAG
948 CTTAAG
BlpI 536 GCTCAGC GC^T[ACGT]AGC
1228 GCTCAGC
Bpu1102I 536 GCTCAGC GC^T[ACGT]AGC
1228 GCTCAGC
BsiHKAI 1437 GAGCAC G[AT]GC[AT]^C
1867 GAGCTC
BsmFI 149 GGGAC GGGAC(10/14)
2043 GGGAC
BsrBI 40 CCGCTC CCGCTC(-3/-3)
1784 CCGCTC
CelII 536 GCTCAGC GC^T[ACGT]AGC
1228 GCTCAGC
KpnI 213 GGTACC GGTAC^C
2212 GGTACC
MspA1I 603 CAGCTG C[AC]G^C[GT]G
867 CAGCTG
NheI 2114 GCTAGC G^CTAGC
2279 GCTAGC
NspI 1069 ACATGC [GA]CATG^[CT]
2092 ACATGC
PvuII 603 CAGCTG CAG^CTG
867 CAGCTG
Tsp45I 427 GTGAC ^GT[GC]AC
480 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI Asp700I BanII BbvI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XmnI