################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AlwI Aor51HI ApaI AscI AseI AsnI Asp718I AvaI AviII AvrII BamHI BbeI BcgI BcnI BfrI BglI BlnI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CfoI ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NciI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccII 1789 CGCG CG^CG AhdI 1127 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1521 GTGCAC G^TGCAC AocI 479 CCTGAGG CC^T[ACGT]AGG ApaLI 1521 GTGCAC G^TGCAC Asp700I 2077 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1127 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 902 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 870 TGGCCA TGG^CCA BanI 1579 GGTGCC G^G[CT][GA]CC BbrPI 332 CACGTG CAC^GTG BbvCI 326 CCTCAGC CCTCAGC(-5/-2) BciVI 733 GTATCC GTATCC(6/5) BclI 3 TGATCA T^GATCA BfaI 956 CTAG C^TAG BglII 1515 AGATCT A^GATCT BlpI 1180 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1180 GCTAAGC GC^T[ACGT]AGC BsaI 1358 GGTCTC GGTCTC(1/5) BsgI 1810 CTGCAC GTGCAG(16/14) BsmBI 1650 GAGACG CGTCTC(1/5) BspHI 753 TCATGA T^CATGA BspLU11I 1935 ACATGT A^CATGT BsrFI 1446 GCCGGC [GA]^CCGG[CT] BstUI 1789 CGCG CG^CG BstYI 1515 AGATCT [GA]^GATC[CT] Bsu36I 479 CCTGAGG CC^T[ACGT]AGG CelII 1180 GCTAAGC GC^T[ACGT]AGC Cfr10I 1446 GCCGGC [GA]^CCGG[CT] DraIII 7 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1127 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 479 CCTGAGG CC^T[ACGT]AGG EcoNI 616 CCTCAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1905 GATATC GAT^ATC FauI 355 GCGGG CCCGC(4/6) FbaI 3 TGATCA T^GATCA FseI 1445 GGCCGGCC GGCCGG^CC HapII 1447 CCGG C^CGG HindIII 1685 AAGCTT A^AGCTT HpaII 1447 CCGG C^CGG MaeI 956 CTAG C^TAG MflI 1515 AGATCT [GA]^GATC[CT] MluNI 870 TGGCCA TGG^CCA MscI 870 TGGCCA TGG^CCA MspI 1447 CCGG C^CGG MvnI 1789 CGCG CG^CG NaeI 1446 GCCGGC GCC^GGC NcoI 1929 CCATGG C^CATGG NgoMIV 1446 GCCGGC G^CCGGC PflFI 902 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1976 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 332 CACGTG CAC^GTG PmlI 332 CACGTG CAC^GTG PstI 266 CTGCAG CTGCA^G PvuII 1945 CAGCTG CAG^CTG RcaI 753 TCATGA T^CATGA SexAI 809 ACCAGGT A^CC[AT]GGT SfcI 266 CTGCAG C^T[GA][CT]AG Tth111I 902 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1976 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 955 TCTAGA T^CTAGA XhoII 1515 AGATCT [GA]^GATC[CT] XmnI 2077 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 1818 AAATTT [GA]^AATT[CT] 2050 GAATTT ApoI 1818 AAATTT [GA]^AATT[CT] 2050 GAATTT BanII 188 GAGCTC G[GA]GC[CT]^C 1109 GAGCTC BsaAI 332 CACGTG [CT]AC^GT[GA] 548 CACGTA BspMI 208 GCAGGT ACCTGC(4/8) 268 GCAGGT EaeI 870 TGGCCA [CT]^GGCC[GA] 1891 CGGCCA EcoT22I 595 ATGCAT ATGCA^T 1508 ATGCAT HphI 989 GGTGA GGTGA(8/7) 6 TCACC MspA1I 854 CCGCTG C[AC]G^C[GT]G 1945 CAGCTG NsiI 595 ATGCAT ATGCA^T 1508 ATGCAT NspI 1935 ACATGT [GA]CATG^[CT] 2040 ACATGC SacI 188 GAGCTC GAGCT^C 1109 GAGCTC SapI 1037 GAAGAGC GCTCTTC(1/4) 665 GCTCTTC ScaI 1116 AGTACT AGT^ACT 1146 AGTACT SmlI 1112 CTCAAG C^T[CT][GA]AG 1139 CTCAAG TfiI 58 GATTC G^A[AT]TC 1972 GAATC Tsp45I 1166 GTCAC ^GT[GC]AC 1500 GTCAC TspRI 922 CACTG CA[GC]TG[ACGT][ACGT]^ 1168 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AfaI AflIII AluI AlwNI AspHI AvaII BbsI BbvI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HgaI HincII HindII HinfI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI StuI StyI TaqI Tru9I TseI Tsp509I TthHB8I XcmI