##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AlwI Aor51HI
ApaI AscI AseI AsnI Asp718I
AvaI AviII AvrII BamHI BbeI
BcgI BcnI BfrI BglI BlnI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CfoI ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI FspI HaeII HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NarI NciI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccII 1789 CGCG CG^CG
AhdI 1127 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1521 GTGCAC G^TGCAC
AocI 479 CCTGAGG CC^T[ACGT]AGG
ApaLI 1521 GTGCAC G^TGCAC
Asp700I 2077 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1127 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 902 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 870 TGGCCA TGG^CCA
BanI 1579 GGTGCC G^G[CT][GA]CC
BbrPI 332 CACGTG CAC^GTG
BbvCI 326 CCTCAGC CCTCAGC(-5/-2)
BciVI 733 GTATCC GTATCC(6/5)
BclI 3 TGATCA T^GATCA
BfaI 956 CTAG C^TAG
BglII 1515 AGATCT A^GATCT
BlpI 1180 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1180 GCTAAGC GC^T[ACGT]AGC
BsaI 1358 GGTCTC GGTCTC(1/5)
BsgI 1810 CTGCAC GTGCAG(16/14)
BsmBI 1650 GAGACG CGTCTC(1/5)
BspHI 753 TCATGA T^CATGA
BspLU11I 1935 ACATGT A^CATGT
BsrFI 1446 GCCGGC [GA]^CCGG[CT]
BstUI 1789 CGCG CG^CG
BstYI 1515 AGATCT [GA]^GATC[CT]
Bsu36I 479 CCTGAGG CC^T[ACGT]AGG
CelII 1180 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1446 GCCGGC [GA]^CCGG[CT]
DraIII 7 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1127 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 479 CCTGAGG CC^T[ACGT]AGG
EcoNI 616 CCTCAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1905 GATATC GAT^ATC
FauI 355 GCGGG CCCGC(4/6)
FbaI 3 TGATCA T^GATCA
FseI 1445 GGCCGGCC GGCCGG^CC
HapII 1447 CCGG C^CGG
HindIII 1685 AAGCTT A^AGCTT
HpaII 1447 CCGG C^CGG
MaeI 956 CTAG C^TAG
MflI 1515 AGATCT [GA]^GATC[CT]
MluNI 870 TGGCCA TGG^CCA
MscI 870 TGGCCA TGG^CCA
MspI 1447 CCGG C^CGG
MvnI 1789 CGCG CG^CG
NaeI 1446 GCCGGC GCC^GGC
NcoI 1929 CCATGG C^CATGG
NgoMIV 1446 GCCGGC G^CCGGC
PflFI 902 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1976 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 332 CACGTG CAC^GTG
PmlI 332 CACGTG CAC^GTG
PstI 266 CTGCAG CTGCA^G
PvuII 1945 CAGCTG CAG^CTG
RcaI 753 TCATGA T^CATGA
SexAI 809 ACCAGGT A^CC[AT]GGT
SfcI 266 CTGCAG C^T[GA][CT]AG
Tth111I 902 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1976 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 955 TCTAGA T^CTAGA
XhoII 1515 AGATCT [GA]^GATC[CT]
XmnI 2077 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 1818 AAATTT [GA]^AATT[CT]
2050 GAATTT
ApoI 1818 AAATTT [GA]^AATT[CT]
2050 GAATTT
BanII 188 GAGCTC G[GA]GC[CT]^C
1109 GAGCTC
BsaAI 332 CACGTG [CT]AC^GT[GA]
548 CACGTA
BspMI 208 GCAGGT ACCTGC(4/8)
268 GCAGGT
EaeI 870 TGGCCA [CT]^GGCC[GA]
1891 CGGCCA
EcoT22I 595 ATGCAT ATGCA^T
1508 ATGCAT
HphI 989 GGTGA GGTGA(8/7)
6 TCACC
MspA1I 854 CCGCTG C[AC]G^C[GT]G
1945 CAGCTG
NsiI 595 ATGCAT ATGCA^T
1508 ATGCAT
NspI 1935 ACATGT [GA]CATG^[CT]
2040 ACATGC
SacI 188 GAGCTC GAGCT^C
1109 GAGCTC
SapI 1037 GAAGAGC GCTCTTC(1/4)
665 GCTCTTC
ScaI 1116 AGTACT AGT^ACT
1146 AGTACT
SmlI 1112 CTCAAG C^T[CT][GA]AG
1139 CTCAAG
TfiI 58 GATTC G^A[AT]TC
1972 GAATC
Tsp45I 1166 GTCAC ^GT[GC]AC
1500 GTCAC
TspRI 922 CACTG CA[GC]TG[ACGT][ACGT]^
1168 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwNI
AspHI AvaII BbsI BbvI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HgaI
HincII HindII HinfI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI StuI
StyI TaqI Tru9I TseI Tsp509I
TthHB8I XcmI